一氧化氮和过氧化氢对万寿菊不定根形成的影响

摘要/Abstract

摘要： 以万寿菊（Tagetes erecta L.）为试材,研究了一氧化氮（NO）和过氧化氢（H2O2）对植物不定根形成中的影响及其相互关系。结果表明,外源NO供体肖普纳（SNP）和H2O2可显著地促进万寿菊外植体不定根的形成,且呈现明显的剂量效应,最适的SNP和H2O2浓度分别为50 μM和200 μM。NO和H2O2共同处理的万寿菊不定根的数量和根长显著高于NO或H2O2单独处理。NO在H2O2诱导万寿菊不定根形成的信号途径中起了重要作用；同时,H2O2包含在NO诱导不定根形成途径中。NO引起万寿菊外植体内源H2O2含量增加,H2O2亦促进内源NO积累。可见,NO和H2O2在万寿菊不定根形成过程中具有协同诱导效应,两者在促进不定根形成过程中可能通过互作反应提高各自的信号水平。

关键词: 一氧化氮, 过氧化氢, 万寿菊, 不定根

Abstract: Effect of nitric oxide (NO) and hydrogen peroxide (H2O2) on adventitious root development of marigold (Tagetes erecta L.) and their interrelationship were studied. Results showed that exogenous NO-donor-sodium nitroprusside (SNP) and H₂O₂, had significant positive effect on adventitious root development, and the effect was dose-dependent with a maximal biological response at 200 μM for H₂O₂ and 50 μM for SNP. The root number and length of explants treated with both H₂O₂ and NO were significantly higher than those of explants treated with H₂O₂ or NO alone. NO is essential for H₂O₂-induced rooting, and H₂O₂ is also involved in NO-induced rooting. NO treatment enhanced H2O2 levels in cells of explants hypocotyls, simultaneously external application of H2O2 induced NO generation in cells too. Thus, H₂O₂and NO may act synergistically to mediate adventitious root generation of the explants and they may enhance each other's levels by their mutual amplification in that process.

Key words: nitric oxide (NO), hydrogen peroxide(H₂O₂), Marigold (Tagetes erecta L.), adventitious root

中图分类号:

Q945

张美玲, 廖伟彪, 肖洪浪. 一氧化氮和过氧化氢对万寿菊不定根形成的影响[J]. 中国沙漠, 2012, 32(1): 105-111.

ZHANG Mei-ling, LIAO Wei-biao, XIAO Hong-lang. Effect of Nitric Oxide and Hydrogen Peroxide on Adventitious Root Development of Marigold[J]. JOURNAL OF DESERT RESEARCH, 2012, 32(1): 105-111.

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