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中国沙漠 ›› 2015, Vol. 35 ›› Issue (6): 1573-1578.DOI: 10.7522/j.issn.1000-694X.2015.0049

• 生物与土壤 • 上一篇    下一篇

基于ISSR分子标记数据的兰州百合核心种质构建方法研究

李谋强1, 师桂英1, 叶树辉2, 黄彦玮1, 边小荣1   

  1. 1. 甘肃农业大学甘肃省作物遗传改良与种质创新实验室/园艺学院, 甘肃 兰州 730070;
    2. 临洮县农技推广中心, 甘肃 临洮 730500
  • 收稿日期:2014-11-23 修回日期:2015-03-23 出版日期:2015-11-20 发布日期:2015-11-20
  • 通讯作者: 师桂英(Email:shigy@gsau.edu.cn)
  • 作者简介:李谋强(1987-),男,甘肃临洮人,硕士研究生,主要从事蔬菜生理及栽培技术研究。Email:915395774@qq.com
  • 基金资助:
    甘肃省高等学校基本科研业务费项目;甘肃省农牧厅农业科技创新项目(GNCX-2013-36)

Methods of Establishing Lanzhou Lily Core Collection Based on ISSR DNA Markers

Li Mouqiang1, Shi Guiying1, Ye shuhui2, Huang Yanwei1, Bian Xiaorong1   

  1. 1. Gansu Key Lab of Crop Improvement And Germplasm Enhancement/College of Hoticulture, Gansu Agricultural University, Lanzhou 730070, Gansu, China;
    2. Lintao Agricultural Extension Center, Lintao 730500, Gansu, China
  • Received:2014-11-23 Revised:2015-03-23 Online:2015-11-20 Published:2015-11-20

摘要: 利用ISSR分析获得的数据,研究了依据Nei & Li、SM和Jaccard遗传距离,采用UPGMA聚类法进行逐步聚类筛选核心种质的效果,并最终采用SM遗传距离逐步聚类从175份兰州百合种质中筛选出了37份核心种质。保留了初始种质21.14%的样品,多态性位点数、多态性位点百分率、Nei's遗传多样性指数和Shannon's信息指数的保留率分别为96.08%、96.59%、105.27%、103.62%,t检验表明核心种质的Nei's遗传多样性指数和Shannon's信息指数与原种质没有显著差异,表明构建的核心种质能很好的代表原始种质的遗传多样性。

关键词: 兰州百合, ISSR, 核心种质, SM遗传距离, UPGMA聚类法

Abstract: In this experiments, we researched the methods of establishing core collection of Lanzhou lily(Lilium davidii Duch.var.unicolor Cotton)based on ISSR data, which was analyzed by UPGMA cluster of Nei&Li, SM and Jaccard genetic distances. The results showed that the method from SM genetic distances was optimal to construct core collection, and by this method, there were 37 accessions were chosen as the core collection, which was 21.14%samples of initial collection, and the reserved information ratio of polymorphic loci,percentage of polymorphic loci, Nei's gene diversity and Shannon's index were 96.08%, 96.59%, 105.27%, 103.62%, respectively. Meanwhile, according to t-test, Nei's gene diversity,Shannon's information of primary core collection was not significantly different from that of the total collection, So the constructed primary core collection could represent the genetic diversity of the total collection.

Key words: Lanzhou lily, ISSR, core collection, SM genetic distances, UPGMA cluster

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