兰州百合(Lilium davidii var.willmottiae)遗传多样性和遗传结构

doi:10.7522/j.issn.1000-694X.2018.00073

摘要/Abstract

摘要： 采用EST-SSR分子标记，对来自7个主要栽培区的兰州百合（Lilium davidii var.willmottiae）种质资源进行遗传多样性分析。结果显示：（1）兰州百合在物种水平上具有较高的遗传多样性，多态性位点百分率（PPB）90.91%，Nei's遗传多样性指数（H）0.3256，Shannon's遗传多样性信息指数（I）0.4875；各栽培居群遗传多样性为西果园 > 榆中 > 魏岭 > 阿干 > 黄峪 > 金沟 > 永靖。（2）兰州百合栽培群体基因分化度G_st为0.2395，基因流（N_m）为1.5876，各栽培居群间遗传分化水平低，遗传差异主要存在于居群内。（3）兰州百合种质材料69个采样点的聚类结果显示，84.05%的材料都聚合到一个类群，这与STRUCTURE遗传结构分析的结果一致，说明兰州百合种质资源的遗传基础相对狭窄。兰州百合种质资源的遗传多样性主要由小部分种质的遗传变异引起，收集研究这些稀有特异种质，将为兰州百合育种改良及优异基因发掘提供重要参考。

关键词: 兰州百合(Lilium davidii var.willmottiae), SSR, 遗传多样性, 遗传结构

Abstract: The genetic diversity of Lilium davidii var. willmottiae selected from seven cultivated cites in Lanzhou was examined with EST-SSR markers. The results revealed a high degree of diversity at the species level, but the genetic differentiation among populations was low. Percentage of polymorphic loci was 90.91%, Nei's gene diversity was 0.3256; Shannon's information index was 0.4875;Gene flow and genetic differentiation coefficient were 1.5876 and 0.2395, respectively. The scale of genetic diversity index of the cultivated populations was as follows, Xiguoyuan > Yuzhong > Weiling > Agan > Huangyu > Jingou > Yongjing. In addition, The UPGMA cluster analysis revealed that 84.05% of materials were clustered into one group, which was consistent with the STRUCTURE results. That illustrated the narrow genetic base of L. davidii var. willmottiae. It was concluded that the genetic diversity of L. davidii var. willmottiae was mainly due to a small amount of genetic variation of germplasm. Therefore, collection and study of rare germplasm resources would provide the scientific grounds to breeding improvement and excavating elite allele gene.

Key words: Lilium davidii var. willmottiae, SSR, genetic diversity, genetic structure

中图分类号:

崔文娟, 林玉红, 石有太, 欧巧明, 罗俊杰. 兰州百合(Lilium davidii var.willmottiae)遗传多样性和遗传结构[J]. 中国沙漠, 2018, 38(6): 1267-1274.

Cui Wenjuan, Lin Yuhong, Shi Youtai, Ou Qiaoming, Luo Junjie. Genetic Diversity and Genetic Structure of Cultivated Lilium davidii var. willmottiae Populations[J]. Journal of Desert Research, 2018, 38(6): 1267-1274.

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