The microbial metabolism activities in the sandy soils of the forests drip-irrigated with different saline water were investigated by Biolog method in this study. The main results are as below: (1) The microbial utilization capability of single carbon source (referred as AWCD value) continuously improved with increase of culturing time, the AWCD value was less than 0.5 and at a slow increasing stage within 48 h, then entered into a logarithm increasing stage after 48 h, a steady stage appeared after 216 h, but a declining stage was not found. (2) The AWCD values decreased notably with increasing salinity of the drip irrigation water, which mainly caused by differences of the microbial utilization capabilities of carbon sources including amine, phenolic compounds and carbohydrate among the different soil samples. (3) Vertical variations of the AWCD values among the different soil layers were significant, the biggest was at 70-100 cm, and the minimum was at the soil surface. (4) The positive correlation coefficients between AWCD vales and soil physical and chemical factors including contents of organism, total nutrient (N, P, K), available nutrient (N, P, K), pH value, cation exchange amount and bulk density, were greater than 0.85. So, the differences of microbial capabilities of utilizing the carbon sources could be subjected to the soil chemical properties; especially to the soil nutrient content and acidity-alkalinity. Amine, phenolic compounds and carbohydrate were regarded as sensitive carbon sources for distinguishing the soil microbial communities from the different drip-irrigated forest lands.
[1] Elsridge D J,Koen T B.Cover and floristics of microphytic soil crusts in relation to indices of landscape health[J].Plant Ecology,1998,137(1):101-114.
[2] 邵玉琴,赵吉.草原沙地微生物结皮与固沙作用的研究[J].农业环境科学学报,2004,23(1):94-97.
[3] Zak C J,Michael R W,Daryl L M,et al.Functional diversity of microbial communities:a quantitative approach[J].Soil Biology & Biochemistry,1994,26(9):1101-1108.
[4] 雷加强,李生宇,靳正忠,等.塔里木沙漠公路防护林生态工程的综合环境效应[J].科学通报,2008,53(增刊II):190-202.
[5] 李生宇,雷加强,徐新文,等.塔里木沙漠公路影响下的地表形态变化[J].科学通报,2006,51(增刊II):101-109.
[6] 李生宇,李红忠,雷加强,等.塔克拉玛干沙漠不同立地条件下咸水滴溉苗木的生长差异[J].水土保持学报,2004,18(3):118-122.
[7] 周智彬,李培军,徐新文,等.塔克拉玛干沙漠腹地人工绿地对沙地盐分时空分布的影响[J].水土保持学报,2002,16(2):16-19.
[8] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.
[9] Choi K,Dobbs F C.Comparison of two kinds of Biolog micro-plates (GN and ECO) in their ability to distinguish among aquatic microbial communities[J].Journal of Microbiology Methods,1999,36(3):203-213.
[10] 王强,戴九兰,吴大千,等.微生物生态研究中基于BIOLOG方法的数据分析[J].生态学报,2010,30(3):817-823.
[11] Garland J L.Analytical approaches to the characterization of samples of microbial communities using patterns of potential C source utilization[J].Soil Biology & Biochemistry,1996,28(2):213-221.
[12] 胡可,王利宾.BIOLOG微平板技术在土壤微生态研究中的应用[J].土壤通报,2007,38(4):819-821.
[13] 郑华,欧阳志云,方治国,等.BIOLOG在土壤微生物群落功能多样性研究中的应用[J].土壤学报,2004,41(3):456-461.
[14] 区余端,苏志尧,彭桂香,等.车八岭山地常绿阔叶林冰灾后土壤微生物群落功能多样性[J].生态学报,2009,29(11):6156-6164.
[15] 郑华,陈法霖,欧阳志云,等.不同森林土壤微生物群落对Biolog-GN板碳源的利用[J].环境科学,2007,28(5):1126-1130.
[16] 张建国,徐新文,雷加强,等.咸水滴灌对沙漠公路防护林土壤环境的影响[J].农业工程学报,2008,24(10):34-39.
[17] 王晓蕾,张琇,周云锋,等.沙漠微生物群落功能多样性分析[J].水土保持通报,2012,32(3):91-95.
[18] Calbrix R,Laval K,Barray S.Analysis of the potential functional diversity of the bacterial community in soil:a reproducible procedure using sole carbon-source utilization profiles[J].European Journal of Soil Biology,2005,41(2):11-20.
[19] 林瑞余,戎红,周军建,等.苗期化感水稻对根际土壤微生物群落及其功能多样性的影响[J].生态学报,2007,27(9):3644-3654.
[20] 陆爽,张霞,谭勇,等.栽培红花生长期土壤微生物与土壤理化因子动态[J].草业科学,2011,28(12):2084-2091.
[21] 李云,孙波,李忠佩,等.不同气候条件对旱地红壤微生物群落代谢特征的长期影响[J].土壤,2011,43(1):60-66.
[22] Rietz D N,Haynes R J.Effects of irrigation-induced salinity and sodicity on soil microbial activity[J].Soil Biology & Biochemistry,2003,35:845-854.
[23] Sudipta T,Sabitri K,Ashis C,et al.Microbial biomass and its activities in salt-affected coastal soils[J].Biology & Fertility of Soils,2006,42:273-277.
[24] Jannike W,Florian W,Rainer G J.Impact of salinity on soil microbial communities and the decomposition of maize in acidic soils[J].Geoderma,2006,137:100-108.
[25] Tripathi S,Kumari S,Chakraborty A,et al.Microbial biomass and its activities in salt-affected coastal soils[J].Biology & Fertility of Soils,2006,42:273-277.
[26] Pankhurst C E,Yu S,Hawke B G.Capacity of fatty acid profiles and substrate utilization patterns to describe differences in soil microbial communities associated with increased salinity or alkalinity at three locations in South Australia[J].Biology & Fertility of Soils,2001,33(3):204-217.
[27] Jonathan Z K,John A B.High incidence of halotolerant bacteria in Pacific hydrothermal-vent and pelagic environments[J].FEMS Microbiology Ecology,2000,32:249-260.
[28] White C,Tardif J C,Adkins A,et al.Functional diversity of microbial communities in the mixed boreal plain forest of central Canada[J].Soil Biology & Biochemistry,2005,37:1359-1372.
[29] O’Donnell A G,Seasman M,Macrae A,et al.Plants and fertilizers as drivers of change in microbial community structure and function in soils[J].Plant & Soil,2001,232(1/2):135-145.
[30] 靳正忠,雷加强,徐新文,等.流沙区不同立地条件下防护林土壤微生物多样性分析[J].中国沙漠,2011,31(6):1430-1436.
[31] 逄蕾,肖洪浪,谢忠奎,等.砂田不同覆盖方式对土壤微生物组成的影响[J].中国沙漠,2012,32(2):351-358.
[32] 靳正忠,雷加强,李生宇,等.流动沙漠腹地防护林土壤肥力质量垂直演化与评价[J].土壤学报,2010,47(6):1075-1085.
甘公网安备 62010202000688号