NaCl胁迫下钙对沙拐枣(Calligonum arborescens)株高及光合特性的影响

王静静; 张文鹏; 徐当会

doi:10.7522/j.issn.1000-694X.2013.00453

中国沙漠 >

2015 , Vol. 35 >Issue 1: 167 - 174

DOI: https://doi.org/10.7522/j.issn.1000-694X.2013.00453

生物与土壤

NaCl胁迫下钙对沙拐枣(Calligonum arborescens)株高及光合特性的影响

王静静 ,
张文鹏 ,
徐当会

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兰州大学生命科学学院/草地农业生态系统国家重点实验室, 甘肃兰州 730000

王静静(1989-),女,河南济源人,硕士研究生,主要从事植物生理生态研究.Email: wangjj12@lzu.edu.cn

收稿日期: 2013-11-08

修回日期: 2013-12-16

网络出版日期: 2015-01-20

基金资助

国家自然科学基金项目(31370423,30900171);中国博士后科学基金特别资助项目(20100340)

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Influence of Calcium on the Plant Height, Photosynthesis, Fluorescence and Chlorophyll Content of Calligonum arborescens under NaCl Stress

Wang Jingjing ,
Zhang Wenpeng ,
Xu Danghui

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State Key Laboratory of Grassland and Agro-ecosystems/school of life Sciences, Lanzhou University, Lanzhou 730000, China

Received date: 2013-11-08

Revised date: 2013-12-16

Online published: 2015-01-20

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摘要

研究了在NaCl胁迫下添加钙盐对沙拐枣(Calligonum arborescens)株高、净光合速率、PSII光化学效率、叶绿素含量等的影响,探讨了外源钙盐对植物在NaCl胁迫下的缓解作用.结果表明:随着NaCl浓度的升高,在未添加钙盐的情况下,植株的株高、净光合速率、F_v/F_m、叶绿素含量均呈下降趋势;8 g·L^-1NaCl浓度时,添加CaCl₂后植株的净光合速率、F_v/F_m、叶绿素含量均高于同浓度Ca(NO₃)₂的处理,而16 g·L^-1NaCl浓度时,Ca(NO₃)₂对NaCl胁迫的缓解作用好于CaCl₂;当钙浓度为5 mM时CaCl₂对应的植株高度高于Ca(NO₃)₂,当钙浓度为10、15、20 mM时Ca(NO₃)₂对株高的作用好.相对于生理指标而言,沙拐枣形态指标对NaCl胁迫不敏感;钙对NaCl胁迫的缓解作用与NaCl浓度有关,即存在一个最佳的钠/钙比值,高过或低于这个比值,钙盐的缓解作用就会下降;CaCl₂和Ca(NO₃)₂对NaCl胁迫缓解作用取决于NaCl的浓度,当植物处于中等程度NaCl胁迫时,CaCl₂对生理胁迫的缓解作用比Ca(NO₃)₂好,若植物所受NaCl胁迫程度比较严重,则Ca(NO₃)₂的缓解作用好.

关键词： NaCl胁迫; 钙盐; 沙拐枣(Calligonum arborescens); 株高; 光合速率; PSII光化学效率; 叶绿素含量

本文引用格式

王静静 , 张文鹏 , 徐当会 . NaCl胁迫下钙对沙拐枣(Calligonum arborescens)株高及光合特性的影响[J]. 中国沙漠, 2015 , 35(1) : 167 -174 . DOI: 10.7522/j.issn.1000-694X.2013.00453

Abstract

NaCl stress can restrain plant growth. Studies have shown that application calcium can alleviate NaCl stress to some extent, so as to promote plant growth. In this paper, we studied the plant height, net photosynthetic, PSII photochemical efficiency, chlorophyll content of Calligonum arborescens under NaCl stress when different calcium sources are added, so as to study the alleviation of NaCl stress by calcium. The results indicate that in the case of not adding calcium salt, plant's height, net photosynthetic, F_v/F_m and chlorophyll content declined with the increasing of sodium chloride concentration; After adding calcium chloride and calcium nitrate, the above indicators all showed a trend of decline after rising first; When NaCl concentration was 8 g·L^-1 the net photosynthetic and F_v/F_m, chlorophyll content of C. arborescens after adding calcium chloride were higher than that of calcium nitrate, however adding calcium nitrate will be better if the NaCl concentration was 16 g·L^-1; When the calcium concentration was 5 mM the plant supplied with CaCl₂ was higher than those supplied with Ca(NO₃)₂, nevertheless, Ca(NO₃)₂ might be a better choice when calcium concentration was 10 mM,15 mM and 20 mM. The above results showed that the morphological indicators relative to the physiological indexes, was not sensitive to NaCl stress. The relief effect of calcium to NaCl stress was related to the concentration of sodium chloride, in other words, there is an optimal ratio of sodium/calcium, higher or lower than which, calcium salt relief effect would fall; The different relief effect between calcium chloride and calcium nitrate depends on the outside of the concentration of NaCl, when plants were under moderate NaCl stress the relief effect of calcium chloride was better than calcium nitrate, however, the calcium nitrate relief well when the plants were under serious NaCl stress.

Key words： NaCl stress; calcium salt; Calligonum arborescens; plant height; net photosynthetic; PSII photochemical efficiency; chlorophyll content

参考文献

[1] Khan M N,Siddiqui M H,Mohammad F,et al.Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defence system and osmoprotectant accumulation[J].Acta Physiologiae Plantorum,2010,32:121-132.
[2] Yao R L,Fang S Z.A strategy of Ca²⁺ alleviating Na⁺ toxicity in salt-treated Cyclocarya paliurus seedlings:photosynthetic and nutritional responses[J].Journal of Plant Growth Regulation,2012,68:351-359.
[3] 王遵亲,祝寿泉,俞仁培.中国盐溃土[M].北京:科学出版社,1993.
[4] Rengasamy P.Transient salinity and subsoil constraints to dryland farming in Australian sodic soils:an overview[J].Australian Journal of Experimental Agriculture,2002,42:351-361.
[5] Yang C W,Chong J N,Li C Y,et al.Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana during adaptation to salt and alkali conditions[J].Plant and Soil,2007,294:263-276.
[6] Renault S.Response of red-osier dogwood (Cornus stolonifera) seedlings to sodium sulphate salinity:effects of supplemental calcium[J].Physiologiaplantarum,2005,123:75-81.
[7] Li R L,Shi F C,Kenji F,et al.Effects of salt and alkali stresses on germination,growth,photosynthesis and ion accumulation in alfalfa (Medicago sativa L.)[J].Soil Science and Plant Nutrition,2010,56:725-733.
[8] Tarchoune I,Degl'Innocenti E,Kaddour R,et al.Effects of NaCl or Na₂SO₄ salinity on plant growth,ion content and photosynthetic activity in Ocimum basilicum L[J].Acta Physiol Plant,2012,34:607-615.
[9] Kader M A,Lindberg S.Cytosolic calcium and pH signaling in plants under salinity stress[J].Plant Signaling & Behavior,2010,5:233-238.
[10] Khan M N,Siddiqui M H,Mohammad F,et al.Interactive role of nitric oxide and calcium chloride in enhancing tolerance to salt stress[J].Nitric Oxide,2012,27:210-218.
[11] 刘建新,王鑫,李博萍.外源一氧化氮供体对盐胁迫下多裂骆驼蓬幼苗光合作用和叶黄素循环的影响[J].中国沙漠,2011,31(1):137-141.
[12] Bledaa F J,Madrida R,García-Torresa A L,et al.Chlorophyll fluorescence and mineral nutrition citrus leaves under salinty stress[J].Journal of Plant Nutrition,2011,34:1579-1592.
[13] Zhang R H,Li J,Guo S R,et al.Effects of exogenous putrescine on gas-exchange characteristics and chlorophyll fluorescence of NaCl-stressed cucumber seedlings[J].Photosynth Res,2009,100:155-162.
[14] Dashti H,Azarnivand H,Shirani H,et al.Response of three Calligonum species to salinity at germination and seedling stages[J].African Journal of Agricultural Research,2011,6(19):4487-4493.
[15] Ding F,Chen M,Sui N,et al.Ca²⁺ significantly enhanced development and salt-secretion rate of salt glands of Limonium bicolor under NaCl treatment[J].South African Journal of Botany,2010,76:95-101.
[16] Genc Y,Tester M,McDonald G K.Calcium requirement of wheat in saline and non-saline conditions[J].Plant and Soil,2010,327:331-345.
[17] Vaghela P M,Patel N T,Pandey I B.Implications of calcium nutrition on the response of Butea monosperma (Fabaceae) to Soil Salinity[J].Anales de Biología,2010,32:15-27.
[18] Gaines A M,Shennan C.Interactive effects of Ca²⁺ and NaCl salinity on the growth of two tomato genotypes differing in Ca²⁺ use effiency[J].Plant Physiology and Biochemistry,1999,37 (7/8) :569-576.
[19] Derbela S,Chaieba M.Germination behaviour and seedling establishment of two desert shrubs,Calligonum polygonoides (Polygonaceae) and Spartidium saharae (Fabaceae),under experimental conditions[J].Acta Botanica Gallica,2007,154(4):533-544.
[20] 康晓珊,潘伯荣,段士民,等.沙拐枣属4种植物同地栽培开花物候与生殖特性比较[J].中国沙漠,2012,32(5):1315-1327.
[21] 苏培玺,赵爱芬,张立新,等.荒漠植物梭梭和沙拐枣光合作用,蒸腾作用及水分利用效率特征[J].西北植物学报,2003,23(1):11-17.
[22] 张利刚,曾凡江,刘波,等.绿洲-荒漠过渡带四种植物光合及生理特征的研究[J].草业学报,2012,21(1):103-111.
[23] 苏培玺,严巧娣.C₄荒漠植物梭梭和沙拐枣在不同水分条件下的光合作用特征[J].生态学报,2006,26(1):75-82.
[24] 朱军涛,李向义,张希明,等.4种荒漠植物的抗氧化系统和渗透调节的季节变化[J].中国沙漠,2011,31(6):1467-1471.
[25] Xu X W,Xu H L,Wang Y L,et al.The effect of salt stress on the chlorophyll level of the main sand-binding plants in the shelterbelt along the Tarim Desert Highway[J].Chinese Science Bulletin,2008,53:109-111.
[26] Liang S M,Yan H L,Zhang X M,et al.Physiological response of natural C.taklimakanensis B.R.Pan et G.M.Shen to unconfined groundwater in the hinterland of the Taklimakan Desert[J].Chinese Science Bulletin,2008,53:112-118.
[27] 李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999:137-263.
[28] 尹增芳,何祯祥,陈梦阳,等.外源钙对NaCl胁迫下海滨锦葵种子萌发和幼苗生长的缓解效应[J].西北植物学报,2006,26(3):462-466.
[29] 刘丽云.氯化钠胁迫下不同钙盐对苦楝萌发的缓解效应[J].湖北农业科学,2009,48(9):2181-2183.
[30] 赵旭,王林权,周春菊,等.钙离子对两种基因型冬小麦萌发过程中盐胁迫效应的影响[J].土壤通报,2006,37(4):748-752.
[31] 王伟华,张希明,闫海龙,等.盐水处理对沙拐枣气体交换特征、光合色素和可溶性糖含量的影响[J].干旱区地理,2009,32(4):597-603.
[32] 韩张雄,李利,徐新文,等.NaCl胁迫对3种荒漠植物幼苗叶绿素荧光参数的影响[J].西北植物学报,2008,28(9):1843-1849.
[33] 刘雪琴,仝瑞建,施佳妮.外源Ca²⁺对盐胁迫下玉米萌发与幼苗生长的影响[J].中国农学通报,2010,26(17):197-200.
[34] 梁洁,严重玲,李裕红,等.Ca(NO₃)₂对NaCl胁迫下木麻黄扦插苗生理特征的调控[J].生态学报,2004,24(5):1073-1077.
[35] Greenway H,Munns R.Mechanisms of salt tolerance in nonhalophytes[J].Annuall Review of Plant Physiology,1980,31:149-190

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