利用ISSR分析获得的数据,研究了依据Nei & Li、SM和Jaccard遗传距离,采用UPGMA聚类法进行逐步聚类筛选核心种质的效果,并最终采用SM遗传距离逐步聚类从175份兰州百合种质中筛选出了37份核心种质。保留了初始种质21.14%的样品,多态性位点数、多态性位点百分率、Nei's遗传多样性指数和Shannon's信息指数的保留率分别为96.08%、96.59%、105.27%、103.62%,t检验表明核心种质的Nei's遗传多样性指数和Shannon's信息指数与原种质没有显著差异,表明构建的核心种质能很好的代表原始种质的遗传多样性。
In this experiments, we researched the methods of establishing core collection of Lanzhou lily(Lilium davidii Duch.var.unicolor Cotton)based on ISSR data, which was analyzed by UPGMA cluster of Nei&Li, SM and Jaccard genetic distances. The results showed that the method from SM genetic distances was optimal to construct core collection, and by this method, there were 37 accessions were chosen as the core collection, which was 21.14%samples of initial collection, and the reserved information ratio of polymorphic loci,percentage of polymorphic loci, Nei's gene diversity and Shannon's index were 96.08%, 96.59%, 105.27%, 103.62%, respectively. Meanwhile, according to t-test, Nei's gene diversity,Shannon's information of primary core collection was not significantly different from that of the total collection, So the constructed primary core collection could represent the genetic diversity of the total collection.
[1] 陈艳华,郭俊琴,张旭东.基于GIS的兰州百合适生种植气候区划[J].干旱气象,2014,32(1):157-161.
[2] 徐学军,梁玉文,胡相莉.百合新品种兰州百合1号[J].中国蔬菜,2010(13):30-31.
[3] 徐学军,梁玉文,刘凯,等.百合新品种兰州百合2号[J].中国蔬菜,2010(20):34.
[4] 刘冬云,张晓曼,杨楠,等.兰州百合和有斑百合的核型研究[J].西北农林科技大学学报(自然科学版),2009,37(11):102-106.
[5] 陈丽梅,李雪,杜捷,等.兰州百合B染色体的初步研究[J].西北植物学报,2003,23(11):2007-2010.
[6] 张彦妮,钱灿.12种百合属植物花粉形态扫描电镜观察[J].草业学报,2011,20(5):111-118.
[7] Li X,Yang C L,Zhang M,et al.Analysis of Protein and Isoenzyme of Lilum davidii var.unicolor,Lilium asiatic Hybrids and their hybrid F1[J].Agricultural Science & Technology,2011,12(1):54-57.
[8] 王丽艳,荆瑞勇.秋水仙碱诱导兰州百合四倍体[J].核农学报,2008,22(5):581-584.
[9] 崔光芬,吴丽芳,吴学尉,等.基于ITS序列分析豹子花属与5种百合的亲缘关系[J].西北植物学报,2008,28(10):1941-1946.
[10] 李恩香,黄玉琴,蒋满英,等.江西省龙牙百合种质资源遗传多样性研究[J].园艺学报,2010,37(5):811-816.
[11] 吴祝华,姜福星,施季森,等.岷江百合生境及遗传多样性[J].东北林业大学学报,2007, 35(7):40-43.
[12] 吴祝华,施季森,席梦利,等.百合种质资源花粉形态及亲缘关系研究[J].浙江林学院学报,2007,24(4):406-412.
[13] 李自超,张洪亮,孙传清,等.植物遗传资源核心种质研究现状与展望[J].中国农业大学学报,1999,4(5):51-62.
[14] Paterson A H,Brubaker C L,Wendel J F.A rapid method for extraction of cotton(Gossypium spp.)genomic DNA suitable for RFLP or PCR analysis[J].Plant Molecular Biology Reporter,1993,11(2):122-127.
[15] Escribano P,Viruel M A,Hormaza J I.Comparison of different methods to construct a core germplasm collection in woody pe-rennial species with simple sequence repeat markers.A case study in cherimoya(Annona cherimola,Annonaceae),an under-utilised sub-tropical fruit tree species[J].Annals of Applied Biology,2008,153(1):25-32.
[16] Ausubel F M.精编分子生物学实验指南[M].颜子颖,王海林 译.北京:科学出版社,1998:36-38.
[17] Hu J,Zhu J,Xu H M.Methods of constructing core collections by stepwise clustering with three sampling strategies based on the genotypic values of crops[J].Theoretical and Applied Genetics,2000,101:264-268.
[18] 刘遵春,刘大亮,崔美,等.整合农艺性状和分子标记数据构建新疆野苹果核心种质[J].园艺学报,2012,39(6):1045-1054.
[19] 刘勇,孙忠海,刘德春,等.利用分子标记选择柚类核心种质资源[J].果树学报,2006,23(3):339-345.
[20] 张春雨,陈学森,张艳敏,等.采用分子标记构建新疆野苹果核心种质的方法[J].中国农业科学,2009,42(2):597-604.
[21] 陈俊百,黄勇,张林,等.利用ISSR分子标记构建山东、河北省区鲁桑地方品种核心种质[J].蚕业科学,2008,34(4):587-591.
[22] 李国强,李锡香,沈镝,等.基于形态数据的大白菜核心种质构建方法的研究[J].园艺学报,2008,35(12):1759-1766.
[23] 刘遵春,刘大亮,崔美,等,整合农艺性状和分子标记数据构建新疆野苹果核心种质[J].园艺学报,2012,39(6):1045-105.
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