碱胁迫下NO和Ca2+对裸燕麦(Avena nuda)幼苗生理特性的影响

刘建新; 王金成; 王瑞娟; 贾海燕

doi:10.7522/j.issn.1000-694X.2015.00023

中国沙漠 >

2016 , Vol. 36 >Issue 3: 695 - 701

DOI: https://doi.org/10.7522/j.issn.1000-694X.2015.00023

生物与土壤

碱胁迫下NO和Ca²⁺对裸燕麦(Avena nuda)幼苗生理特性的影响

刘建新 ,
王金成 ,
王瑞娟 ,
贾海燕

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陇东学院生命科学与技术学院/甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000

刘建新(1964-),男,甘肃通渭人,教授,主要从事植物逆境生理生态研究。E-mail:liujx1964@163.com

收稿日期: 2014-08-09

修回日期: 2015-02-06

网络出版日期: 2016-05-20

基金资助

甘肃省庆阳市科技计划项目(KZ2014-19)

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Effects of Exogenous Nitric Oxide and Ca²⁺ on Physiological Characteristics of Avena nuda Seedlings under Alkali Stress

Liu Jianxin ,
Wang Jincheng ,
Wang Ruijuan ,
Jia Haiyan

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College of Life Science and Technology/University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, Longdong University, Qingyang 745000, Gansu, China

Received date: 2014-08-09

Revised date: 2015-02-06

Online published: 2016-05-20

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摘要

为了探讨NO对碱胁迫下裸燕麦(Avena nuda)生理响应的调节作用,采用营养液培养,研究Ca²⁺对外源NO供体硝普钠(SNP)所诱导的NaHCO₃胁迫下裸燕麦品种"定莜6号"幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明:25μmol·L^-1SNP或6.5 mmol·L^-1CaCl₂能够缓解75 mmol·L^-1NaHCO₃胁迫对幼苗生长的抑制作用,叶片超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和质膜H⁺-ATP酶活性较单独NaHCO₃胁迫处理明显增强,O₂^·-、H₂O₂、丙二醛含量显著下降,抗坏血酸、可溶性糖、可溶性蛋白质、脯氨酸含量和K⁺/Na⁺显著提高;添加SNP的同时增添Ca²⁺通道抑制剂La³⁺或Ca²⁺螯合剂EGTA抑制了SNP的这些作用。Ca²⁺在NO诱导的碱胁迫下裸燕麦活性氧清除能力和渗透溶质含量提高、维持离子平衡中起重要作用,NO的作用可能依赖于胞浆Ca²⁺的浓度。

关键词： NO; 裸燕麦(Avenanuda); 碱胁迫; 活性氧代谢; 渗透溶质

本文引用格式

刘建新 , 王金成 , 王瑞娟 , 贾海燕 . 碱胁迫下NO和Ca²⁺对裸燕麦(Avena nuda)幼苗生理特性的影响[J]. 中国沙漠, 2016 , 36(3) : 695 -701 . DOI: 10.7522/j.issn.1000-694X.2015.00023

Abstract

To understand the regulative effect of exogenous nitric oxide on physiological response of naked oat (Avena nuda) seedlings under alkali stress, through nutrient solution cultivating in greenhouse, the naked oat cultivar, Dingyou No.6', was selected as the plant material and sodium nitroprusside (SNP) was used as nitric oxide donor to investigate the effects of Ca²⁺ on growth, reactive oxygen metabolism and osmotica accumulation of naked oat seedlings induced by exogenous nitric oxide under NaHCO₃ stress. The results showed that 25 μmol·L^-1SNP or 6.5 mmol·L^-1 CaCl₂ could alleviate the inhibition of 75 mmol·L^-1 NaHCO₃ stress to the seedlings growth significantly, and the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and plasma membrane H⁺-ATPase in leaves were enhanced, the contents of O₂^·-, H₂O₂, malondialdehyde were decreased, and the contents of ascorbic acid, soluble sugar, soluble protein and proline as well as K⁺/Na⁺ ratio were also increased significantly, in respectively. The effect of SNP was decreased after treatment of blocker of plasma membrane Ca²⁺ channel La³⁺ or extracellular Ca²⁺ chelant EGTA. The above results indicated that Ca²⁺ might play an important role in reactive oxygen metabolism, osmotica accumulation and balance of Na⁺ and K⁺ induced by exogenous nitric oxide in naked oat under alkali stress. The effect of nitric oxide here de pends on the content of Ca²⁺ in cytoplasm.

Key words： nitric oxide; Avena nuda; alkali stress; reactive oxygen metabolism; osmotica

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