In order to explore the physiological mechanism of exogenous NO to improve the cold resistance of Poa effects of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on growth, osmotic adjustment and antioxidant system of seedlings of Poa under low temperature stress which was simulated by artificial turf on wild Poa pratensis and Poa sphondylodes were studied. Results showed that with the SNP concentration increasing, the aboveground biomass, growth rate, the content of soluble sugar (SS) and soluble protein (SP), the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of the two tested materials first increased and then decreased in the concentration range of sodium nitroprusside which was 0-700 mol·L-1 under the low temperature stress. The relative membrane permeability, the content of malondialdehyde (MDA) and free proline (Pro) trended conversely. It was harmful in high concentration of NO while low concentration of NO had a protective effect. Among all the NO experimental treatments, 100 μmol·-1 showed the best alleviation effect on cold-resistance, the lowest level of membrane lipid peroxidation, accumulated the greatest amount of osmotic regulation substances, and the highest activity of active-oxygen-scavenging enzyme. The alleviation of exogenous NO in P. sphondylodes Trin. was more sensitive than that of Poa pratensis. under low temperature stress.
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