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  • ISSN 1000-694X
  • 双月刊 创刊于1981年
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生物与土壤

外源NO对野生早熟禾幼苗抗寒性的影响

  • 陈润娟 ,
  • 雷娅伟 ,
  • 白小明 ,
  • 田彦锋
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  • 甘肃农业大学 草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070
陈润娟(1990-),女,甘肃灵台人,硕士研究生,主要从事草坪科学方面的研究。E-mail:353362662@qq.com

收稿日期: 2017-01-13

  修回日期: 2017-03-31

  网络出版日期: 2017-11-20

基金资助

国家自然科学基金项目(31560667);甘肃农业大学校级自列课题(GSAU-ZL-2015-054)

Effect of NO on Cold Resistance of Several Wild Kentucky Bluegrass Germplasm Materials

  • Chen Runjuan ,
  • Lei Yawei ,
  • Bai Xiaoming ,
  • Tian Yanfeng
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  • College of Grassland Science/Key Laboratory of Grassland Ecosystem of Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Gansu Agricultural University Lanzhou 730070, China

Received date: 2017-01-13

  Revised date: 2017-03-31

  Online published: 2017-11-20

摘要

以野生草地早熟禾(Poa pratensis)和硬质早熟禾(Poa sphondylodes)为材料,通过人工室内模拟低温逆境胁迫,研究外源一氧化氮(NO)供体亚硝基铁氰化钠(硝普钠,SNP)对低温胁迫下野生早熟禾幼苗生长、渗透调节和抗氧化系统的影响,探讨外源NO提高早熟禾抗寒性的生理机制。结果表明,低温胁迫下:0~700 μmol·L-1浓度范围内,随SNP浓度的增大,两个供试材料的地上生物量和生长速度先增大后减小,相对膜透性、丙二醛(MDA)含量、游离脯氨酸(F-Pro)含量先减小后增大,可溶性糖(SS)和可溶性蛋白(SP)含量先增加后减小,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性先增强后减弱;高浓度NO产生伤害,低浓度NO则有保护作用;SNP浓度100 μmol·L-1时,膜脂过氧化水平最低,渗透调节物质积累最多,保护酶活性最强,对低温胁迫的缓解效果最佳。硬质早熟禾对低温胁迫下外源NO的缓解较草地早熟禾敏感。

本文引用格式

陈润娟 , 雷娅伟 , 白小明 , 田彦锋 . 外源NO对野生早熟禾幼苗抗寒性的影响[J]. 中国沙漠, 2017 , 37(6) : 1171 -1179 . DOI: 10.7522/j.issn.1000-694X.2017.00029

Abstract

In order to explore the physiological mechanism of exogenous NO to improve the cold resistance of Poa effects of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on growth, osmotic adjustment and antioxidant system of seedlings of Poa under low temperature stress which was simulated by artificial turf on wild Poa pratensis and Poa sphondylodes were studied. Results showed that with the SNP concentration increasing, the aboveground biomass, growth rate, the content of soluble sugar (SS) and soluble protein (SP), the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of the two tested materials first increased and then decreased in the concentration range of sodium nitroprusside which was 0-700 mol·L-1 under the low temperature stress. The relative membrane permeability, the content of malondialdehyde (MDA) and free proline (Pro) trended conversely. It was harmful in high concentration of NO while low concentration of NO had a protective effect. Among all the NO experimental treatments, 100 μmol·-1 showed the best alleviation effect on cold-resistance, the lowest level of membrane lipid peroxidation, accumulated the greatest amount of osmotic regulation substances, and the highest activity of active-oxygen-scavenging enzyme. The alleviation of exogenous NO in P. sphondylodes Trin. was more sensitive than that of Poa pratensis. under low temperature stress.

参考文献

[1] Delledonne M,Xia Y,Dixon R A,et al.Nitric oxide functions as a signal in plant disease resistance[J].Nature,1998,394:585-588.
[2] Dumer J,Klessig D F.Nitric oxide as a signal in plants[J].Current Opinion Plant Biology,1999,2:369-374.
[3] 陈银萍,王晓梅,杨宗娟,等.NO对低温胁迫下玉米种子萌发及幼苗生理特性的影响[J].农业环境科学学报,2012,31(2):270-277.
[4] Zhang W L,Shen W B,Xu L L.Effect of nitric oxide on the signal molecularity[J].Chemistry of Life,2002,22:61-62.
[5] 刘建新,胡浩斌,王鑫.外源一氧化氮供体对镉胁迫下黑麦草幼苗活性氧代谢、光合作用和叶黄素循环的影响[J].环境科学学报,2009,29(3):626-633.
[6] 俞玲,马晖玲.甘肃几种早熟禾内源激素水平及干旱适应性[J].中国沙漠,2015,35(1):182-188.
[7] 池春玉,丁国华,连永权,等.低温胁迫对三种冷季型草坪草脯氨酸含量及膜透性的影响[J].中国农学通报,2007,23(1):101-104.
[8] Lamattina L,Beligni M V,Garcia M C.Method of Enhancing the Metabolic Function and the Growing Conditi-ons of Plants and Seeds:United States,US6242384B1[P].2001.
[9] Liu X,Wang L,Liu L,et al.Alleviating effect of exogenous nitric oxide in cucumber seedling against chilling stress[J].African Journal of Biotechnology,2011,10(21):4380-4386.
[10] 张华,沈文飚,徐朗莱.一氧化氮对渗透胁迫下小麦种子萌发及其活性氧代谢的影响[J].Acta Botanica Sinica,2003,45(8):901-905.
[11] 黄忠勤,杨峰,樊继德,等.一氧化氮对汞胁迫下小麦种子萌发及抗氧化代谢的影响[J].安徽农业科学,2011,39(9):5161-5163.
[12] 阮海华,沈文飚,叶茂炳,等.一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应[J].科学通报,2001,46(23):1993-1997.
[13] 孟艳艳,范术丽,宋美珍,等.NO对生长发育中棉花叶片NO含量及其对抗氧化物酶的影响[J].作物学报,2011,37(10):1828-1836.
[14] 肖强,陈娟,吴飞华,等.外源NO供体硝普钠(SNP)对盐胁迫下水稻幼苗中叶绿素和游离脯氨酸含量以及抗氧化酶的影响[J].作物学报,2008,34(10):1849-1853.
[15] 朱涵毅,陈益军,劳佳丽,等.外源NO对镉胁迫下水稻幼苗抗氧化系统和微量元素积累的影响[J].生态学报,2013,33(2):603-609.
[16] 刘柿良,潘远智,杨容孑,等.外源一氧化氮对镉胁迫下长春花质膜过氧化、ATPase及矿质营养吸收的影响[J].植物营养与肥料学报,2014,20(2):445-458.
[17] 王光辉,李江,郑岩,等.外源CO及NO对干旱胁迫下水稻糊粉层细胞死亡的影响[J].西北植物学报,2014,34(1):13-20.
[18] 周万海,冯瑞章,师尚礼,等.NO对盐胁迫下苜蓿根系生长抑制及氧化损伤的缓解效应[J].生态学报,2015,35(11):3606-3614.
[19] 马向丽,魏小红,龙瑞军,等.外源一氧化氮提高一年生黑麦草抗冷性机制[J].生态学报,2005,25(6):1269-1274.
[20] 梁慧敏,夏阳,杜峰,等.低温胁迫对草地早熟禾抗性生理生化指标的影响[J].草地学报,2001,9(4):283-286.
[21] 邹琦.植物生理学试验指导[M].北京:中国农业出版社,2000.
[22] Urbanek H,Kuzniakgebarowska E.Elicitation of defence responses in bean leaves by Botrytis cinerea polygalacturonase[J].Acta Physiologiae Plantarum,1991,13:20-28.
[23] 杨美森,王雅芳,干秀霞,等.外源一氧化氮对冷害胁迫下棉花幼苗生长、抗氧化系统和光合特性的影响[J].中国农业科学,2012,45(15):3058-3067.
[24] 樊怀福,杜长霞,朱祝军.外源NO对低温胁迫下黄瓜幼苗生长、叶片膜脂过氧化和光合作用的影响[J].浙江农业学报,2011,23(3):538-542.
[25] 黄治远,李隆华,张云贵,等.龙眼叶片可溶性糖含量与耐寒性的关系[J].西南园艺,2004,32(4):18-19.
[26] Jian L C.Advances of the studies on the mechanism of plant cold hardiness[J].Chinese Bulletin of Botany,1992,12(3):17-22.
[27] 汤红玲,李江,陈惠萍.外源一氧化氮对香蕉幼苗抗冷性的影响[J].西北植物学报,2010,30(10):2028-2033.
[28] 吴雁斌,王一航,张武,等.PEG与SNP对低温下马铃薯试管苗相关生理指标的影响[J].核农学报,2010,24(3):645-649.
[29] 吴锦程,陈伟建,蔡丽琴,等.外源NO对低温胁迫下枇杷幼果抗氧化能力的影响[J].林业科学,2010,46(9):73-78.
[30] 王芳,常盼盼,陈永平,等.外源NO对镉胁迫下玉米幼苗生长和生理特性的影响[J].草业学报,2013,22(2):178-186.
[31] Beligni M V,Lamattina L.Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues[J].Planta,1999,208(3):337-344.
[32] 王罗霞,赵志光,王锁民.一氧化氮对水分胁迫下小麦叶片活性氧代谢及膜脂过氧化的影响[J].草业学报,2006,15(4):104-108.
[33] 张艳艳,刘俊,刘友良.一氧化氮缓解盐胁迫对玉米生长的抑制作用[J].植物生理与分子生物学学报,2004,30(4):455-459.
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