| Biology and Soil |
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| Osmotic Adjustment Mechanism of Cynanchum komarovii under Drought Stress |
| CHEN Cui-yun1,2, ZHAO Xin1,2, LI Xin-rong1,2 |
1.Laboratory of Stress Physiology and ecology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
2.Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China |
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Abstract Cynanchum komarovii is one of the desert plants with drought resistance. To study its osmotic adjustment mechanism under drought stress, the calluses of Cynanchum Komarovii were treated with PEG-6000 at different concentration (0~25%), and then content of the osmotic adjustment substances (K+, Na+, proline, betaine, soluble sugars and so on) were measured. Results showed that with the rise of PEG-6000 concentration, the contents of proline and soluble sugars increased obviously (P<0.05). Under drought stress, the contents of fructose and sucrose increased at high concentration PEG-6000 treatments, while the content of trehalose increased very obviously at all PEG-6000 treatments, with 31% enhancement at 10% PEG-6000 treatment and 98% at 25% PEG-6000 treatment, respectively. The correlation analysis showed that the coefficient between trehalose content and PEG-6000 concentration was the highest with R2=0.9437 (P<0.01). These results suggest that trehalose is the primary osmotic adjustment substance of Cynanchum komarovii resisting to drought stress.
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Received: 30 January 2012
Published: 20 September 2012
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| [1]李新荣,张志山,王新平,等.干旱区土壤植被系统恢复的生态水文学研究进展[J].中国沙漠,2009,29(5):845-852.[2]杨丽雯,周海燕,樊恒文,等.沙坡头人工固沙植被生态系统土壤恢复研究进展[J].中国沙漠,2009,29(6):1116-1123.[3]Wang Q H,Li F G,Zhang X,et al.Purification and characterization of a CkTLP protein from Cynanchum komarovii seeds that confers antifungal activity[J].Plos One,2011,6(2):e16930.[4]Sun H L,Blanford S,Guo Y Y,et al.Toxicity and influences of the alkaloids from Cynanchum komarovii AL. Iljinski (Asclepiadaceae) on growth and cuticle components of Spodoptera litura Fabricius (Noctuidae) larvae[J].Natural Product Research,2012,26(10):903-912.[5]张永康,赵德惠,李勇.牛心朴子的研究进展[J].农业科学研究,2007,28(1):52-55.[6]李玉美.牛心朴子草的研究进展及应用前景[J].内蒙古石油,2008,3:29-31.[7]Harb A,Krishnan A,Ambavaram M M R,et al.Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth[J].Plant Physiology,2010,154:1254 -1271.[8]史胜青.梭梭干旱诱导表达基因及其调控研究[D].中国林业科学研究院,2006.[9]Tran L-S P,Nakashima K,Shinozaki K,et al.Plant gene networks in osmotic stress response:From genes to regulatory networks[J].Methods in Enzymology,2007,428:109-128.[10]王蕊,孙广玉.干旱对大豆叶片葫芦巴碱含量和渗透调节的影响[J].中国沙漠,2010,30(3):552-555.[11]Song J,Feng G,Tian C Y,et al.Osmotic adjustment traits of Suaeda physophora,Haloxylon ammdoendron and Haloxylon persicum in field or controlled conditions[J].Plant Science,2006,170:113-119.[12]任庆成,杨铁钊,刘培玉,等.植物抗旱性研究进展[J].中国农学通报,2009,25(15):76-79.[13]张海燕.NaCl胁迫对滨藜生长及其根和叶中无机离子含量的影响[J].武汉植物学研究,2001,19(5):409-415.[14]谭会娟,贾荣亮,刘玉冰,等.NaCl胁迫下红砂愈伤组织中主要离子累积特征的研究[J].中国沙漠,2010,30(6):1305-1310.[15]董伊晨,刘悦秋.土壤水分对异株荨麻（Urtica dioica）保护酶和渗透调节物质的影响及其与叶片光合和生物量的相关性[J].生态学报,2009,29(6):2845-2851.[16]杜金伟,崔世茂,金丽萍,等.水分胁迫对山杏渗透调节物质积累及保护酶活性的影响[J].内蒙古农业大学学报,2009,30(2):88-93.[17]刘瑞冬,王有年,王丽雪,等.外源甜菜碱对仁用杏抗旱生理指标的影响[J].内蒙古农业大学学报,2004,2:69-72.[18]韩蕊莲,李丽霞,梁宗锁.干旱胁迫下沙棘叶片细胞膜透性与渗透调节物质研究[J].西北植物学报,2003,23(1):23-27.[19]吴银明,王平,刘洪升,等.NaCl分根胁迫对羊草幼苗生长及其生理生化特性的影响[J].西北植物学报,2007,27(9):1807-1813.[20]朱军涛,李向义,张希明,等.4种荒漠植物的抗氧化系统和渗透调节的季节变化[J].中国沙漠,2011,31(6):1467-1471.[21]米海莉,许兴,李树华,等.干旱胁迫下牛心朴子幼苗的抗旱生理反应和适应性调节机理[J].干旱地区农业研究,2002,20(4):11-16.[22]李树华,许兴,米海莉,等.水分胁迫对牛心朴子植株生长及渗透调节物质积累的影响[J].西北植物学报,2003,23(4):592-596.[23]周海燕,李新荣,樊恒文,等.极端条件下几种锦鸡儿属灌木的生理特性[J].中国沙漠,2005,25(2):182-190.[24]张殿忠,汪沛洪,赵会贤.测定小麦叶片游离脯胺酸的含量的方法[J].植物生理学通讯,1990,4:62-65.[25]杨秀英,王书林.葸酮-硫酸比色法测定枇杷叶中可溶性多糖的含量[J].亚太传统医药,2008,4(8):26-27.[26]张自萍,郭荣,廖国玲,等.枸杞甜菜碱含量测定方法的比较研究[J].西北农业学报,2007,16(6):292-295.[27]Zhao L,Zhang F,Guo J,et al.Nitric oxide functions as a signa in salt resistance in the calluses from two ecotypes of reed[J].Plant Physiology,2004,134:849-857.[28]孙萍,段喜华.干旱胁迫对长春花光合特性及可溶性糖的影响[J].东北林业大学学报,2010,38(8):54-56.[29]张美云,钱吉,郑师章.渗透胁迫下野生大豆游离脯氮酸和可溶性糖的变化[J].复旦学报,2002,40(5):558-561.[30]王蕊,李新国,李绍鹏,等.干旱胁迫下2种香蕉幼苗叶片和根的主要渗透调节物质的变化[J].基因组学与应用生物学,2010,29( 3):518-522.[31]杨洪兵,韩振海,许雪峰.NaCl和等渗PEG对苹果属植物可溶性糖含量的影响[J].莱阳农学院学报,2005,22(2):105-107.[32]李书华,李仲芳,陈封政,等.孑遗植物可溶性糖的种类及含量[J].湖北农业科学,2009,48(6):1477-1478.[33]赵昕,吴雨霞,赵敏桂,等.NaCl胁迫下盐芥和拟南芥渗透调节作用响应[J].兰州大学学报,2006,42:117-123.[34]唐建平,王正鹏,张树珍,等.高等植物果聚糖分类和功能的研究进展[J].海南大学学报(自然科学版),2009,27(3):308-312.[35]郭蓓,胡磊,何欣,等.海藻糖-6-磷酸合成酶转基因烟草提高耐盐性的研究[J].植物学通报,2008,25(1):41-49.[36]Vandesteene L,Ramon M,LeRoy K,et al.A single active trehalose-6-P synthase (TPS) and a family of putative regulatory TPS-like proteins in Arabidopsis[J].Molecular Plant,2010,3:406-419.[37]杜丽璞,徐惠君,叶兴国,等.小麦转TPS基因植株的获得及其初步功能鉴定[J].麦类作物学报,2007,27(3):369-373.[38]贾炜珑,胡鸢雷,张彦芹,等.海藻糖合酶基因转化黑麦草及耐旱性研究[J].分子植物育种,2007,5(1):27-31. |
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2012, Vol. 32 
