| Biology and Soil |
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| Difference in Photosynthetic Response of Ryegrass Seedling to NaCl and NaHCO3 Stress |
| LIU Jian-xin, WANG Xin, JIA Hai-yan, LI Dong-bo |
| College of Life Science and Technology/University Provincial Key Laboratory of Protection and Utilization of Longdong Bio-resources in Gansu Province, Longdong University, Qingyang 745000, Gansu, China |
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Abstract To explore the differences of photosynthetic responses of forage to saline stress and alkaline stress, ryegrass seedlings were exposed to Hoagland's nutrient solution at five NaCl or NaHCO3 levels (0, 50, 100, 150, 200 mmol\5L-1), and the effects of NaCl and NaHCO3 stress on plant growth, photosynthetic pigment content, gas exchange and chlorophyll fluorescence parameters, and the activities of Hill reaction and ATPase in chloroplast of ryegrass seedling leaves were investigated by a sandy culture in greenhouse. Results show: (1) With increase of NaCl and NaHCO3 concentration, the whole plant dry weight, the leaf chlorophyll and carotenoid contents, net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr) of the ryegrass seedlings decrease significantly. Intercellular CO2 concentration (Ci) decreases significantly and stomatal limited value (Ls) increases significantly with increasing of NaCl concentration when the NaCl concentration is below 100 mmol\5L-1, and the Ci increases significantly and Ls decreases significantly with further increase of NaCl concentration. While the Ci increases significantly and Ls decreases significantly with the increasing of NaHCO3 concentration. The decrements or increments of all the indexes under NaHCO3 stress were greater than under NaCl stress. (2) The PSII maximal photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPSⅡ), photochemical quenching (qP), and the activities of Hill reaction and ATPase in chloroplast of ryegrass seedling leaves decrease significantly with increasing of NaCl and NaHCO3 stress intensity, while PSII non-photochemical quenching (NPQ) increases significantly, and the ratio of absorbed light energy allocating to the photochemical reaction of PSⅡ (P) reduces significantly, meanwhile, the proportion allocated in antenna heat dissipation (D) increases significantly. At the same concentration of treatments, the decreases of the Fv/Fm, ΦPSII, qP, P, Hill reaction and ATPase activities, and the increases of NPQ and D are higher in NaHCO3 stress than in NaCl stress, suggesting that the injury of NaHCO3 stress to ryegrass seedlings is greater than NaCl stress. All the results show that the tolerance of ryegrass to alkaline stress is lower than to saline stress, which may be concerned with the greater decrease of photosynthetic pigment and the more serious injury of photosynthetic system caused by high pH of alkaline stress.
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Received: 29 January 2012
Published: 20 September 2012
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2012, Vol. 32 
