| Biology and Soil |
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| Optimization of Tissue Culture Techniques for a Drought-resistant Longya 11 Variety of Flax |
| ZHAO Wei, DANG Zhan-hai, LI Wen-juan |
| Crop Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China |
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Abstract To solve problems in tissue culture of flax, such as few embryoid induced from callus, difficult in plant regenerating and rooting, we used Longya 11, a new improved drought-resistant variety of flax, as experiment material to systematically study and eventually optimize the formulas of medium, screening of explants and rooting culture. Results show that the callus incubated with MS+NAA+6-BA at different concentrations presents as dark green color, compact and good mass, but little vitrification or clarified phenomena. The mean and maximum embryoid induction rate and the average seedling survival rate all are improved than the before, with the highest embryoid induction rate and seedling survival rate in the medium of MS+NAA(1.0 mg\5L-1) +6-BA(0.5 mg\5L-1). Besides, there is also notable increase in rooting rate, mean rooting number and mean root length when flax seedlings incubated with medium of 1/2 MS+AC+NAA+PP333, and the optimal medium for rooting culture is 1/2MS+AC(0.05%)+NAA(0.5 mg\5L-1)+PP333(0.001 mg\5L-1). In this medium the seedlings have more branch roots and adventitious roots, which can help the seedlings to absorb water and mineral salt from soil, and improve the seedling survival rate. There are increases in the embryoid induction rate and seedling survival rate in the optimized medium formula, but the number of plant differentiated from embryoid is still relatively low, and this is unfavorable to massive transplants of flax, so a further research on the subject is urgent.
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Received: 20 June 2012
Published: 20 September 2012
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| [1]康庆华,关凤芝,王玉福,等.中国亚麻分子育种研究进展[J].中国农业科学,2006,39(12):2428-2434.[2]王利民,张建平,米君,等.国外引进油用亚麻品种资源农艺性状分析与评价[J].中国油料作物学报,2011,33(4):356-361.[3]党占海,赵蓉英,王敏,等.国际视野下胡麻研究的可视化分析[J].中国麻业科学,2010,32(6):305-307.[4]Dai Q,Franke A A,Jin F,et al.Urinary excretion of phytoestrogens and risk of breast cancer among Chinese women in shanghai[J].Cancer Epidemiology,Biomarkers and Prevention,2002,11:815-821.[5]Adlercrcutz H.Phyto-oestrogens and cancer[J].The Lancet Oncology,2002,3(6):364-373. [6]Thompson L U,Rickard S E,Orcheson L J,et al.Flaxseed and its lignan and oil components reduce mammary tumor growth at a late stage of carcinogenesis[J].Carcinogenesis,1996,17:1373-1376.[7]Thompson L U,Seidl M M,Rickard S E,et al.Antitumorgenic effect of a mammalian lignan precursor from flaxseed[J].Nutrition and Cancer,1996,26:159-165.[8]Prasad K.Antioxidant activity of secoisolariciresinol diglucosidc-drived metabolites,sccoisolariciresinol,enterodiol,and enterolactone[J].International Journal of Angiology,2000,9(4):220-225.[9]Prasad K.Oxidative stress as a mechanism of diabetes in diabetic BB prone rats:Effect of secoisolariciresinol diglucoside (SDG)[J].Molecular and Cellular Biochemistry,2000,209:89-96.[10]赵利,党占海,李毅,等.亚麻籽的保健功能和开发利用[J].中国油脂,2006,31(3):71-73.[11]郭永利,范丽娟.亚麻籽的保健功效和药用价值[J].中国麻业科学,2007,29(3):147-149.[12]蔡迪华,王润元,郭铌,等.民勤春小麦NDVI与产量的关系及对气候变暖的响应[J].中国沙漠,2010,30(2):376-382.[13]周瑞莲,孙国钧,王海鸥.沙生植物渗透调节物对干旱、高温的响应及其在抗逆性中的作用[J].中国沙漠,1999,19(S1):18-22.[14]周瑞莲,赵彦宏,赵哈林,等.钠盐对冬小麦抗旱性增效作用调控机理的生理生态学分析[J].中国沙漠,2012,32(3):784-792.[15]余丽琴,钟梓璘,王记林,等.生物技术的发展及其在农作物育种中的应用[J].江西农业学报,2009,21(9):8-12. [16]孙丽华,朱翔.生物技术在林木遗传改良中的应用与进展[J].四川林业科技,2000,21(2):53-56.[17]王玉富,周思君,刘燕,等.利用农杆菌介导法进行亚麻转基因的培养基研究[J].中国麻作,2000,22(1):14-16.[18]薄天岳,叶华智,王世全,等.亚麻抗锈病基因M4的特异分子标记[J].遗传学报,2002,29(10):922-927.[19]毛国杰,刘建华,任勇,等.亚麻抗锈病的分子基础[J].植物病理学报,2000,30(3):200-206.[20]薄天岳,叶华智,李小兵,等.亚麻抗枯萎病基因FuJ7(t)的分子标记[J].中国农业科学,2003,36(3):287-291.[21]康庆华.亚麻转基因中抗生素应用效果的研究[J].中国麻业,2005,27(2):94-97.[22]张杰道,孟祥兵.植物分子育种方法研究进展[J].生命科学研究,2001,5(3):165-169.[23]贝丽霞,李海燕,王子录,等.农作物分子育种的研究现状和展望[J].黑龙江八一农垦大学学报,1997,9(2):29-33.[24]赵玮,党占海,张建平,等.胡麻胚状体诱导及植株再生的研究[J].北方园艺,2010,21:174-176. [25]颉瑞霞,张建平,党占海,等.激素和低温预处理对胡麻花药愈伤组织诱导的影响[J].分子植物育种,2009,7(6):1180-1185.[26]孙上峰,王树彦.提高胡麻花药培养中愈伤组织诱导率的关键技术研究[J].内蒙古农业科技,2009,3:36-38.[27]高晶,那日,朝鲁蒙,等.电晕电场将大豆Gy3基因导入花棒愈伤组织及植株再生[J].中国沙漠,2011,31(5):1202-1207.[28]谭会娟,李新荣,赵昕,等.红砂愈伤组织适应盐胁迫的渗透调节机制研究[J].中国沙漠,2011,31(5):1119-1123.[29]孙兰弟,管利萍,张颖聪,等.沙生濒危物种霸王的高频植株再生研究[J].中国沙漠,2009,29(2):312-315.[30]钟宇,张健,罗承德,等.观赏植物快繁技术研究概况[J].四川农业大学学报,2002,20(3):295-298.[31]曹孜义,刘国民.实用组织培养技术教程[M].兰州:甘肃科学技术出版社,1996. |
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2012, Vol. 32 
