Abstract:

The structure and diversity characteristics of bacterial and fungal communities in the rhizosphere soil of Phragmites australis were analyzed and compared by high-throughput sequencing technique. The rhizosphere soil of P. australis in four sandy habitats （semi-fixed dune windward slope， semi-fixed dune leeward slope， fixed dune， and flat land） was studied in the oasis-desert transition zone of Hexi Corridor. The results showed that the diversity of microbial communities in the rhizosphere soil of P. australis in the four sandy habitats was significantly different， and the OUT number of bacterial and fungal communities were the highest in fixed dunes. Proteobacteria， Actinomycetes， Firmicutes， Bacteroidetes and Acidobacteria were the main dominant bacterial groups in the rhizosphere soil of reed， among which Proteobacteria and Actinomycetes had the highest relative abundance. The dominant groups in fungal community were Ascomycetes and Basidiomycetes， among which Ascomycetes are the main dominant phyla. The physicochemical properties of rhizosphere soil of P. australis in different sandy habitats were different： The content of organic matter， alkali-hydrolyzed nitrogen and available phosphorus increased with the increase of the fixation degree of sand dunes， all of which were the highest in flat land， the second in fixed sand dunes， the lower in leeward slope and the smallest in windward slope；The soil pH， the content of organic matter， alkali-hydrolyzed nitrogen and available phosphorus significantly affected the diversity of bacterial and fungal communities in the rhizosphere soil of P. australis（P<0.05）.

Key words: Phragmites australis, rhizosphere soil, high throughput sequencing, microbial community