Cloning and expression analysis of Glucose-6-phospho 1 epimerase SpG6P1E in Stipagrostis pennata

doi:10.7522/j.issn.1000-694X.2024.00011

Journal of Desert Research ›› 2024, Vol. 44 ›› Issue (4): 126-136.DOI: 10.7522/j.issn.1000-694X.2024.00011

Cloning and expression analysis of Glucose-6-phospho 1 epimerase SpG6P1E in Stipagrostis pennata

Rui Tang¹(), Shan Yin¹, Kailu Chen¹, Yuetao Li¹, Linyuan Cheng¹, Jingru Wang¹, Fei Wang¹^,²^,³(), Rong Li¹^,²^,³(), Hongbin Li¹^,²^,³

^1.College of Life Science /, Shihezi University，Shihezi 832003，Xinjiang，China
^2.Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology /, Shihezi University，Shihezi 832003，Xinjiang，China
^3.Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University，Shihezi 832003，Xinjiang，China

Received:2023-12-04 Revised:2024-01-05 Online:2024-07-20 Published:2024-08-29
Contact: Fei Wang,Rong Li

Abstract

Abstract:

Glucose-6-phospho 1 epimerase （G6P1E） plays an important role in plant growth and development and stress response. The purpose of this study is to clone and analyze the expression characteristics of Stipagrosis penataSpG6P1E gene， and lay a foundation for further exploring the mechanism and function of its influence on the development of S. Pennata root sand trap. The full-length open reading frame sequence of S. pennataSpG6P1E gene was cloned by molecular cloning technology. SpG6P1E was cloned from the desert plant S. pennata， which includes a 325 amino acids. SpG6P1E was localized in the cytoplasm. Sequence and evolutionary analysis showed that this gene contains a aldose_epim domain， which is closely related to orthologous gene of monocotyledon plants. Subcellular localization analysis showed that the gene was located in cytoplasm and involved in cytoplasmic glucose metabolism. The results of qRT-PCR showed that SpG6P1E expression had a close link with rhizosheath development and soluble sugar content. The expression of SpG6P1E gene was significantly induced by abiotic stress. Protein interaction and annotation analysis indicated that SpG6P1E might affect the content of soluble sugar by participating in a series of processes such as sugar synthesis and sugar metabolism. The results of this study provide a foundation for in-depth investigation of the functions of G6P1E genes and the mechanisms by which they regulate plant tissue development and adaptation to stress.

Key words: Stipagrostis pennata, Glucose-6-phospho 1 epimerase, SpG6P1E, bioinformatics analysis, subcellular localization, rhizosheath, abiotic stress

CLC Number:

Q943

Rui Tang, Shan Yin, Kailu Chen, Yuetao Li, Linyuan Cheng, Jingru Wang, Fei Wang, Rong Li, Hongbin Li. Cloning and expression analysis of Glucose-6-phospho 1 epimerase SpG6P1E in Stipagrostis pennata[J]. Journal of Desert Research, 2024, 44(4): 126-136.

Figures/Tables 9

Fig.1 Plant and rhizosheaths of Stipagrostis pennata

Table 1 Primers sequences

引物名称	引物序列	引物功能
SpG6P1E-F	ATGGCCGCCGCGACGTCGTCGT	基因克隆
SpG6P1E-R	ACCCTGAAGAAGAACCTTTTCTGGA	基因克隆
SpG6P1E-1300-F	GAGAGGACAGGGTACCATGGCCGCCGCGACGTCGTCGT	亚细胞定位
SpG6P1E-1300-R	GTACTAGTGTCGACTCTAGAACCCTGAAGAAGAACCTTTTCTGGA	亚细胞定位
SpG6P1E-qRT-F	AAGCCTCCTAAAGCAATCCG	荧光定量
SpG6P1E-qRT-R	GTTTACTGGGAAAGGTGGGG	荧光定量
Actin-F	GAAGATCACTGCCTTGCTCC	内参基因
Actin-R	CGATAACAGCTCCTCTTGGC	内参基因

Fig.2 Sequence feature analysis of SpG6P1E

Fig.3 Sequence conservative analysis of SpG6P1E

Fig.4 G6P1E family domain and motif analysis

Fig.5 The qRT-PCR expression analysis of SpG6P1E

Fig.6 Contents of soluble sugar in rhizosheath development

Fig.7 Subcellular localization of SpG6P1E

Fig.8 Protein interaction network and annotation analysis

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[3]	QIU Dong, WU Nan, ZHANG Yuan-ming, PAN Hui-xia. Ecological Regulation of Stipagrostis pennata by Rhizosheath Microhabitat [J]. JOURNAL OF DESERT RESEARCH, 2012, 32(6): 1647-1654.

Cloning and expression analysis of Glucose-6-phospho 1 epimerase SpG6P1E in Stipagrostis pennata

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