Effect of Complex Saline-alkali Stress on Nitrogen and Phosphorus Absorption and Physiological Traits of Alfalfa Seedlings

Hao Feng; Liu Xiaojing; Zhang Xiaolei; Qi Minxing

doi:10.7522/j.issn.1000-694X.2014.00179

2015 , Vol. 35 >Issue 5: 1268 - 1274

DOI: https://doi.org/10.7522/j.issn.1000-694X.2014.00179

Effect of Complex Saline-alkali Stress on Nitrogen and Phosphorus Absorption and Physiological Traits of Alfalfa Seedlings

Hao Feng ,
Liu Xiaojing ,
Zhang Xiaolei ,
Qi Minxing

Expand

College of Grassland Science/Key Laboratory of Grassland Ecosystem of Ministry of Education/Sino-U.S.Centers for Grazing land Ecosystem Sustainability, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2014-11-04

Revised date: 2014-12-28

Online published: 2015-09-20

Fold

Abstract

The effect of complex saline-alkali stress on nitrogen and phosphorus absorption and physiological characteristics of alfalfa seedlings were studied with Alfalfa (Medicago sativa, Gannong No.3). Seedlings were stressed with different concentrations of neural salts (NaCl and Na₂SO₄) and alkaline salts (NaHCO₃ and Na₂CO₃), altogether 5 combinations: A(NaCl:Na₂SO₄=1:1), B(NaCl:NaHCO₃=1:1), C(NaCl:Na₂SO₄:NaHCO₃:Na₂CO₃=1:1:1:1), D(Na₂SO₄:Na₂CO₃=1:1)、E(NaHCO₃:Na₂CO₃=1:1), and 4 concentrations (25, 50, 100 and 150 mmol·L^-1) in a sand culture experiment. Results show that with the increase of salt concentration, nodule nitrogenase activity and the content of total nitrogen has dropped, and the content of total phosphorus has no obvious change, while proline (Pro), malondialdehyde (MDA), soluble sugar and superoxide dismutase (SOD) content increased. At the same level of the salt concentration, the content between the groups of higher order: E>D>C>B>A.The change ranges of tested indices have reached significant level under the high salt concentration(150 mmol·^-1), in addition to the total phosphorus content.The results suggested that the structure components of alfalfa was damaged under the hybrid salinity-alkalinity stress,reduced nitrogen fixation ability and decreased nitrogen content. Along with the rising of salt concentrations, plant can improve the content of proline and soluble sugar in the body to maintain cell water potential, reduce the injury to the plant cell osmotic stress.

Key words： alfalfa (Medicago sativa); salt and alkali mixed stress; nitrogenase activity; physiological index

Cite this article

Hao Feng , Liu Xiaojing , Zhang Xiaolei , Qi Minxing . Effect of Complex Saline-alkali Stress on Nitrogen and Phosphorus Absorption and Physiological Traits of Alfalfa Seedlings[J]. Journal of Desert Research, 2015 , 35(5) : 1268 -1274 . DOI: 10.7522/j.issn.1000-694X.2014.00179

References

[1] 王志才,牙库甫江,阿西木,等.水盐胁迫对花花柴种子萌发的影响[J].中国沙漠,2012,32(3):750-755.
[2] 李彬,王志春,孙志高,等.中国盐碱地资源与可持续利用研究[J].干旱地区农业研究,2005,23(2):154-158.
[3] 张建锋,宋玉民,邢尚军,等.盐碱地改良利用与造林技术[J].东北林业大学学报,2002,30(6):125-129.
[4] 付爱红,陈亚宁,李卫红,等.砂田集雨补灌对西瓜产量和土壤水分的影响[J].中国沙漠,2005,25(5):744-749.
[5] 高文俊,徐静,谢开云,等.Na₂CO₃和NaHCO₃胁迫下冰草的生长及生理响应[J].草业学报,2011,20(4):299-304.
[6] 李得禄,王继和,李爱德,等.3种驼绒藜属植物种子萌发期耐盐性试验研究[J].中国沙漠,2006,26(6):1009-1014.
[7] 杨科,张保军,胡银岗,等.混合盐碱胁迫对燕麦种子萌发及幼苗生理生化特性的影响[J].干旱地区农业研究,2009,27(3):188-191.
[8] 陈年来,马国军,张玉鑫,等.甜瓜种子萌发和幼苗生长对NaCl胁迫的响应[J].中国沙漠,2006,26(5):814-819.
[9] 王保平,董晓燕,董宽虎.盐碱胁迫对紫花苜蓿幼苗生理特性的影响[J].草地学报,2013,21(6):1124-1129.
[10] 李源,刘贵波,高洪.等.紫花苜蓿种质耐盐性综合评价及盐胁迫下的生理反应[J].草业学报,2010,19(4):79-86.
[11] 闫永庆,刘兴亮,王崑.等.白刺对不同浓度混合盐碱胁迫的生理响应[J].植物生态学报,2010,34 (10):1213-1219.
[12] 霍平慧,李剑峰,师尚礼,等.盐胁迫对超干处理苜蓿种子萌发及幼苗生长的影响[J].草原与草坪,2011,31(1):13-18.
[13] Hoagland D R,Arnon D I.The water culture method for growing plants without soil[J].California Agricultural Experiment Station Bulletin,1950,347:139.
[14] Zaied K A.Kosba Z A.Nassef M A.et al.Induction of Rhizobium Inoculants Harboring Salicylic Acid Gene[J].Australian Journal of Basic and Applied Sciences,2009,3(2):1386-1411.
[15] 谭志远,彭桂香,徐培智,等.普通野生稻(Oryza rufipogon)内生固氮菌多样性及高固氮酶活性[J].科学通报,2009,54(13):1885-1893.
[16] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2006.
[17] Giannopolitis C N,Ries S K.Superoxide dismutases.I.Occurrence in higher plants[J].Plant Physiology,1977,59:309-314.
[18] de Azevedo Neto A D,Prisco J T,Enéas-Filho J,et al.Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants[J].Journal of Plant Physiology,2005,162:1114-1122.
[19] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000:147-170.
[20] 任继周,吴自立,李绶章,等.草原生态化学实习实验指导[M],北京:农业出版社,1987.
[21] 胡发成.种植苜蓿改良培肥地力的研究初报[J].草业科学,2005,22(8):47-49.
[22] Abdel Wahab A M,Shabeb M S A,Younis M A M.Studies on the effect of salinity drought stress and soil type on nodule activities of Lablab purpureus (L) sweet (Kashrangeeg)[J].Jounal of Arid Environment,2002,51(4):587-602.
[23] 尤崇杓.固氮酶的结构和功能研究进展[J].农业生物技术学报,1995,3(1):1-13.
[24] Serraj R,Sinelair T R.N:fixation response to drought in common bean (Phaseolus vulgaris L.)[J].Annal Botany,1998,82(2):229-234.
[25] Sabu A,Sheeja T E,Nambison P.Comparison of praline accumulation in callus and seedlings of two cultivation of Oryza sativa L.differing in salt tolerance[J].Indian Journal of Experimental Biology,1995,33(2):139-141.
[26] Haro R,Baneulos M A,Quintero F J,et al.Genetic basis of sodium tolerance in yeast a model for plants[J].Physiological Plant,1993,89:868-874.
[27] 张永锋,梁正伟,隋丽,等.盐碱胁迫对苗期紫花苜蓿生理特性的影响[J].草业学报,2009,18(4):230-235.
[28] Jaleel C A,Gopi R,Sankar B,et al.Studies on germination,seedling vigour,lipid peroxidation and prolinem etabolism in Catharanthus roseus seedlings under salt stress[J].South African Journal of Botany,2007,73(2):190-195.
[29] 龙明秀,许岳飞,何学青,等.NaCI胁迫下紫花苜蓿幼苗抗氧化酶活性的研究[J].草地学报,2012,20(1):83-87.
[30] 陈冠宜,周美利,孟国花,等.NaCI和Na₂CO₃对盐地碱蓬和小花碱茅幼苗生长的比较研究[J].现代农业科技,2010(22):260-262.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References