Callus Induction and Proliferation in the Endemic Recretohalophyte Reaumuria trigyna

Dang Zhenhua; Qin Xiaochun; Wang Yingchun

doi:10.7522/j.issn.1000-694X.2015.00099

Journal of Desert Research >

2015 , Vol. 35 >Issue 5: 1262 - 1267

DOI: https://doi.org/10.7522/j.issn.1000-694X.2015.00099

Callus Induction and Proliferation in the Endemic Recretohalophyte Reaumuria trigyna

Dang Zhenhua ,
Qin Xiaochun ,
Wang Yingchun

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1. Key Laboratory of Herbage & Endemic Crop Biotechnology in Inner Mongolia, College of Life Science, Inner Mongolia University, Hohhot 010021, China;
2. Baotou Landscape Research Institute,Baotou 014010, Inner Mongolia, China

Received date: 2015-05-14

Revised date: 2015-07-02

Online published: 2015-09-20

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Abstract

The aim of this study was to evaluate callus induction and proliferation in the endangered recretohalophyte Reaumuria trigyna. Mature seeds and seedlings at different development stages were used as the ex-plant materials. The best medium for callus formation and proliferation was improved MSO (pH 5.8) supplemented with 3.0 mg·L^-1 2,4-dichlorophenoxyacetic acid, 1.0 mg·L^-1 naphthaleneacetic acid, 0.5 mg·L^-1 6-benzylaminopurine, 30 g·L^-1 sucrose, 0.5 g·L^-1 casein hydrolysate, and 6.0 g·L^-1 agar. On this medium, the callus induction rate was greater than 70%, and the growth rate was higher than those of ex-plants on other media. The optimal ex-plants for callus induction were hypocotyls from 7-day-old and 25-day-old seedlings. The calli that formed from these ex-plants were loose, light yellow, and showed a lower browning rate, compared with calli that grew from other ex-plants. The optimum duration of the subculture cycle was 30-40 days. With longer passage times, the callus grew faster and was a lighter color. When calli were cultured on media with different NaCl concentrations, their growth was accelerated on media containing 50 and 100 mmol·L^-1 NaCl, but higher concentrations of NaCl inhibited callus growth or resulted in callus death.

Key words： Reaumuria trigyna; callus; induction; proliferation

Cite this article

Dang Zhenhua , Qin Xiaochun , Wang Yingchun . Callus Induction and Proliferation in the Endemic Recretohalophyte Reaumuria trigyna[J]. Journal of Desert Research, 2015 , 35(5) : 1262 -1267 . DOI: 10.7522/j.issn.1000-694X.2015.00099

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