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  • CN 62-1070/P
  • ISSN 1000-694X
  • Bimonthly 1981
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Effects of Exogenous Nitric Oxide and Ca2+ on Physiological Characteristics of Avena nuda Seedlings under Alkali Stress

  • Liu Jianxin ,
  • Wang Jincheng ,
  • Wang Ruijuan ,
  • Jia Haiyan
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  • College of Life Science and Technology/University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, Longdong University, Qingyang 745000, Gansu, China

Received date: 2014-08-09

  Revised date: 2015-02-06

  Online published: 2016-05-20

Abstract

To understand the regulative effect of exogenous nitric oxide on physiological response of naked oat (Avena nuda) seedlings under alkali stress, through nutrient solution cultivating in greenhouse, the naked oat cultivar, Dingyou No.6', was selected as the plant material and sodium nitroprusside (SNP) was used as nitric oxide donor to investigate the effects of Ca2+ on growth, reactive oxygen metabolism and osmotica accumulation of naked oat seedlings induced by exogenous nitric oxide under NaHCO3 stress. The results showed that 25 μmol·L-1 SNP or 6.5 mmol·L-1 CaCl2 could alleviate the inhibition of 75 mmol·L-1 NaHCO3 stress to the seedlings growth significantly, and the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and plasma membrane H+-ATPase in leaves were enhanced, the contents of O2·-, H2O2, malondialdehyde were decreased, and the contents of ascorbic acid, soluble sugar, soluble protein and proline as well as K+/Na+ ratio were also increased significantly, in respectively. The effect of SNP was decreased after treatment of blocker of plasma membrane Ca2+ channel La3+ or extracellular Ca2+ chelant EGTA. The above results indicated that Ca2+ might play an important role in reactive oxygen metabolism, osmotica accumulation and balance of Na+ and K+ induced by exogenous nitric oxide in naked oat under alkali stress. The effect of nitric oxide here de pends on the content of Ca2+ in cytoplasm.

Cite this article

Liu Jianxin , Wang Jincheng , Wang Ruijuan , Jia Haiyan . Effects of Exogenous Nitric Oxide and Ca2+ on Physiological Characteristics of Avena nuda Seedlings under Alkali Stress[J]. Journal of Desert Research, 2016 , 36(3) : 695 -701 . DOI: 10.7522/j.issn.1000-694X.2015.00023

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