Differential gene expression response to warming of typical plants in the Horqin Sandy Land
Received date: 2025-05-06
Revised date: 2025-07-08
Online published: 2025-08-18
This study focused on representative trees， shrubs and herbsin Horqin Sandy Land （Ulmus pumila var. sabulosa， Populus simonii， Artemisia halodendron， Caragana microphylla， Setaria viridis， and Artemisia scoparia）. Using transcriptome sequencing via the Illumina platform， combined with differential gene expression analysis， weighted gene co-expression network analysis （WGCNA）， and functional enrichment methods， we systematically analyzed the molecular response mechanism of different plant life forms to elevated temperature stress. Key findings include： （1） The MYB， AP2/ERF， and GRAS transcription factor families dominate the regulation of heat stress response signals in six tested plants. MYB genes exhibited high connectivity in co-expression networks， potentially coordinating antioxidant and stress response genes to combat high temperatures. （2） Arbor species （e.g.， Populus simonii） showed minimal gene expression changes， relying on structural adaptations （e.g.， cuticle thickening） to mitigate heat stress， while shrubs and herbs upregulated photosynthetic pathway genes （e.g.， PetA） and activated jasmonic acid signaling to adapt to environmental fluctuations. （3） The photosystem II core protein PsbA was upregulated in all species， yet there are differences in the motif arrangement and expression magnitude. The unique PsbA structure in Setaria viridis is correlate with its C4 plant photosynthetic efficiency. （4） Jasmonic acid signaling was prominently activated in herbs but negatively regulated in arbor species， suggesting life-form-driven divergence in hormonal response strategies.
Key words： Horqin Sandy Land; transcriptome; gene expression; warming
Shangbin Shi , Wenda Huang , Hailun Yu , Jing Feng , Yuanzhong Zhu . Differential gene expression response to warming of typical plants in the Horqin Sandy Land[J]. Journal of Desert Research, 2025 , 45(4) : 343 -356 . DOI: 10.7522/j.issn.1000-694X.2025.00217
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