不同有机溶剂萃取生物结皮中叶绿素a效率的比较研究

摘要/Abstract

摘要： 参照水体中浮游植物叶绿素a的萃取测定方法，运用丙酮、乙醇、二甲基甲酰胺（DMF）、二甲基亚砜（DMSO）4种有机溶剂对不同土质不同发育阶段自然和人工生物结皮中叶绿素a的萃取效率进行了比较研究。结果发现，不同有机溶剂从生物结皮中萃取叶绿素a的效率存在极显著差异（P<0.001），而乙醇法的离散度最低；取样量对萃取效率总体上没有显著影响（P>0.05），但在具体的测定中与萃取效率之间存在负相关。4种方法中，DMSO法对藻类占优势的结皮萃取率最高，DMF法对自然结皮的萃取率仅次于DMSO法，在人工结皮中最低；乙醇法和丙酮法的萃取效率普遍较低，而且与结皮属性之间没有明显规律。

关键词: 有机溶剂, 萃取, 生物结皮, 叶绿素a

Abstract: Based on the extraction and measurement of phytoplanktonic Chl-a in water, this study compared contents of Chl-a extracted by acetone, ethanol, N,N-Dimethylformamide (DMF) and Dimethyl Sulphoxide (DMSO) in natural and man-made biological crusts that were in different soil texture and stages of development. The results showed that there was significant statistical difference in content of Chl-a extracted by different organic solvents (P<0.001), while scatteration was the least in ethanol method. Although the sample content hadnt prominent effects on content of Chl-a extraction as a whole (P>0.05), there was a negative correlation between content of Chl-a extraction and sample content. Comparisons displayed that in the algae dominative crusts, content of Chl-a extraction was the highest in DMSO method, while lower in DMF than DMSO method in natural crusts, but the lowest in the man-made crusts. Generally, the extraction rate was lower in acetone and ethanol method, which also hadnt evident relation to the crusts attributes.

Key words: organic solvent, extraction, biological crust, Chl-a

中图分类号:

Q93-31

兰书斌;刘永定;胡春香. 不同有机溶剂萃取生物结皮中叶绿素a效率的比较研究[J]. 中国沙漠, 2009, 29(3): 524-528.

LAN Shu-bin;LIU Yong-ding;HU Chun-xiang. Comparison of Contents of Chlorophyll-a Extracted by Different Organic Solvents in Biological Crusts[J]. JOURNAL OF DESERT RESEARCH, 2009, 29(3): 524-528.

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