低温对百合鳞茎内源激素含量变化的影响

摘要/Abstract

摘要： 以东方百合Siberia和Tiber为试验材料,进行恒温和变温的低温处理,采用高效液相色谱法测定在不同冷藏解除休眠时期赤霉素(GA3)、吲哚乙酸(IAA)、脱落酸(ABA)和玉米素(ZT)4种内源激素含量。结果表明,恒温处理Tiber先解除休眠,同样处理同期种植的百合鳞茎,Tiber比Siberia一般早出苗7 d左右；变温处理比恒温处理ABA含量降低,说明变温处理对百合鳞茎解除休眠有促进作用,可以缩短解除休眠需要的时间,一般变温处理的比恒温处理的早8 d出苗,且变温处理有利于百合生长量的增加,Tiber茎粗和株高分别增加了23.1%、10.1%,同时Siberia分别增加了5.9%和16.6%。变温处理GA3含量Tiber高于恒温处理,Siberia低于恒温处理,品种之间对温度处理的响应有差异。百合鳞茎休眠的解除与IAA/ABA、ZT/ABA、GA3/ABA比值有关,变温Siberia和Tiber的3种比值都在60 d后逐渐升高,说明冷藏60 d可作为鳞茎解除休眠的临界点,为进一步研究百合鳞茎的休眠机理提供了重要线索。

关键词: 低温, 鳞片, 休眠, 内源激素

Abstract: Contents of four endogenous hormones, namely Zeatin(ZT), Gibberellin(GA3), Indoleacetic Acid (IAA), Abscisic Acid (ABA), in Oriental Lily of Siberia and Tiber under constant low temperature and variable low temperature treatments were determined using High Performance Liquid Chromatography. Results show that Tiber relieves dormancy first under constant low temperature treatment, and it emerges 7 d earlier than Siberia; ABA content under variable low temperature treatment is less than under constant low temperature treatment, and it shows that variable low temperature treatment can promote the relieving of dormancy and shorten the time required for relieving dormancy of the lily bulbs. Lily emerges 8 d earlier under variable low temperature treatment than under constant low temperature treatment. Lily grows better under variable low temperature treatment than under constant low temperature treatment, and increments of stem diameter and plant height of Tiber increases are 23.1% and 10.1%, respectively, while that of Siberia are 5.9% and 16.6%, respectively. GA3 content in Tiber under variable low temperature treatment is higher than under constant low temperature treatment, while GA3 content in Siberia under variable low temperature treatment is lower than under constant low temperature treatment. Dormancy release of Lily Bulb is related to ratios of IAA/ABA, ZT/ABA, GA3/ABA, and the three ratios of Siberia and Tiber increase gradually under variable low temperature treatment of 60 d, indicating 60 d of low temperature can be used as the critical point for dormancy relieving of Lily Bulb. The paper provides a clue for further study of the dormancy release mechanism of Lily Bulb.

Key words: low temperature, bulb, dormancy, endogenous hormones

中图分类号:

Q945

李翊华;安丽萍;李彬;谢忠奎*. 低温对百合鳞茎内源激素含量变化的影响[J]. 中国沙漠, 2011, 31(5): 1208-1214.

LI Yi-hua;AN Li-ping;LI Bin;XIE Zhong-kui. Effects of Low Temperature on Change of Endogenous Hormone Contents in Lily Bulb[J]. JOURNAL OF DESERT RESEARCH, 2011, 31(5): 1208-1214.

参考文献

[1]洪波.百合花卉的研究综述[J].东北林业大学学报,2000,28(2):68-70.
[2]夏宜平,高晓晨.试论百合等球根花卉的商品种球国产化问题[C]//高俊平,姜伟贤.中国花卉科技进展——第二届全国花卉科技信息交流会论文集.北京:中国农业出版社,2001:216-223.
[3]Zheng H J,Huang C H,Xia Y P.Studies on the bulb development and its physiological mechanisms in Lilium oriental hybrids[J].Acta Horticulturae,2005,673:91-98.
[4]路宁娜,崔现亮,王桔红,等.不同贮藏条件和光照对5 种蒺藜科植物种子萌发的影响[J].中国沙漠,2008,28(6):1130-1135.
[5]张燕,徐建华,吕光辉.西北干旱区新疆水资源足迹及利用效率动态评估[J].中国沙漠,2008,28(4):775-778.
[6]孟丽红,陈亚宁,李卫红.新疆塔里木河流域水资源承载力评价研究[J].中国沙漠,2008,28(1):185-189.
[7]杨玉海,陈亚宁,李卫红,等.准噶尔盆地西北缘新垦绿洲土地利用对土壤养分变化的影响[J].中国沙漠,2008,28(1):94-96.
[8]Gude H,Verbruggen J J M,Franssen J M,et al.Physiological markers for lily bulb maturity[J].Acta Horticulturae,2000,517:343-350.
[9]Langens-Gerrits M,Hol T,Croes T,et al.Dormancy breaking in lily bulblets regenerated in vitro:effectson growth after planting[J].Acta Horticulturae,1997,430:429-436.
[10]Kim E Y,Choi I D,Park K,et al.Production of non．dormant bulblets of Lilium Oriental Hybrid by control of culture temperature and growth regulators in vitro[J].Journal of the Korean Society for Horticultural Science,2000,41(1):78-82.
[11]Miller W B,Hammer I.Reversed greenhouse temperature alter carbohydrate status in Lilium longiflorum Thunb."Nellie White"[J].Journal of the America Society for Horticultural Science,1993,118(6):736-740.
[12]Miler W B.Localization of reserve mobilization during scalet formation on Easter lily scales[J].Acta Horticulturae,1990,266):95-100.
[13]Miler W B,Langhans W.Low temperature alters carbohydrate metabolism in Easter lily bulbs[J].Hort Science,1990,25(4):463-465.
[14]Miler W B,Langhans W.Carbohydrate changes of Easter lilies during growth in normal and reduced irradiance environments[J].Journal of the America Society for Horticultural Science,1989,114(2):310-315.
[15]Shin K S,Chakrabarty Y.Sprouting rate,change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vitro[J].Scientia Horticulturae,2002,96):195-204.
[16]Takayama T,Toyomnsu T H.Identification of gibberelins and abscisic acid in bulbs of Lilium elegans Thunb.and their quantitative changes during cold treatment and subsequent cultivation[J].Journal of the Japanese Society for Horticultural Science,1993,63(2):189-196.
[17]Djilianov D,Gerrits M A.ABA content and sensitivity during the development of dormancy in lily bulblets regenerated in vitro[J].Physiologia Plantarum,1994,91(4):639-644.
[18]李云飞,孙红梅,李天来.百合鳞茎低温解除休眠过程中酚类物质含量及相关酶活性变化[J].中国农业科学,2004,37(11):1777-1782.
[19]李云飞,孙红梅,李天来.百合鳞茎低温贮藏期间游离氨基酸含量及组分变化[J].植物生理学通讯,2004,40(4):414-418.
[20]李云飞,孙红梅,李天来.不同贮藏温度下兰州百合种球淀粉代谢与萌发关系初探[J].园艺学报,2004,31(3):337-342.
[21]李云飞,孙红梅,李天来.百合鳞茎发育过程中碳水化合物含量及淀粉酶活性变化[J].植物研究,2005,25(1):59-60.
[22]李云飞,孙红梅,李天来.内源ABA对兰州百合鳞茎顶芽内物质变化的调节作用[J].林业科学,2006,42(10):
[23]陈国仓,王洪亮,张承烈,等.河西走廊不同旱生植物内源激素和叶水势的比较研究[J].中国沙漠,1994,14(1):17-20.
[24]陈贵林,葛会波,李青云,等.草莓休眠过程中内源激素含量的变化[J].园艺学报,1998,25(1):89-90.
[25]Rees A R.Charles-Edwards D A.A simple model for the cold requirement of the tulip[J].Annals of Botany,1974,38(155):401-408.
[26]Moe R,Wickstr m A.The effect of storage temperature on shoot growth,flowering and carbohydrate metabolism in tulip bulbs[J].Physiologia Plantarum,1973,28):81-87.
[27]王建峰,孙红梅,李天来,李云飞.百合鳞茎低温处理效应初报[J].沈阳农业大学学报,2003,34(3):169-172.
[28]朱四易,胡正梅,宇文强.伊贝母生长发育周期的研究[J].植物学报,1980,22(1):22-26.
[29]秦振栋,李春俭.低温处理过程中伊贝母鳞茎中若干生理生化变化与休眠解除的关系[J].西北植物学报,1987,7(1):23-28.
[30]张金政,罗丽兰,石雷.低温对解除百合鳞茎休眠和促进开花的作用[J].园艺学报,2007,34(2):517-524.
[31]周燮,李宗霆.植物激素及其免疫检测技术[M].南京:江苏科学技术出版社,1996:
[32]Aung L H,De Hertogh A A.Temperature regulation of growth and endogenous abscisic abscisic acid-like content of Tulipa gesnerianna L[J].Plant Physiology,1979,63):1111-1116.
[33]刘奎彬,李秉真,刘梦云.不同贮藏温度下马铃薯的萌芽和内源激素含量变化[J].植物生理学通讯,1999,35(2):105-107.
[34]Roh S M.Dormancy and maturity in the bulblet of Lilium lancifolium:the influence of growth regulator treatment on the grow and flowering response[J].Journal of the Korean Society for Horticultural Science,1982,23(1):59-63.
[35]Hill L,Reimholz R M.The onset of suerose accumulation in cold stored potato tubers is caused by an increased rate of sucrose synthesis and coincides with low levels of hexose phosphates,an activation of sucrose phosphate synthase and the appearance of a new form of amylase[J].plant cell and environment,1996,19:1223-1237.
[36]Choi S T,Jung W Y.Ahn H G,et al.Effects of duration of cold treatment and planting depth on growth and flowering of Lilium spp[J].Journal of the Korean Society for Horticultural Science,1998,39(6):765-770.
[37]李云飞,孙红梅,李天来.低温解除休眠过程中兰州百合中部鳞片物质变化的生理机制[J].中国农业科学,2005,38(2):376-382.
[38]Ohkawa K.Effects of gibberellins and benzy ladenine on dormancy and flowering of Lilium speciosum[J].Scientia Horticulturae,1979,10(3):255-260.
[39]Endo Y,Arisaka E,Niimi Y.Effects of chilling and GA3treatments on breaking dormancy in Lilium rubellan baker bulblets cultured in vitro[J].Journal of the Japanese Society for Horticultural Science,1988,57(2):250-257.
[40]Niimi Y,Pathania N S,Sindhu S S.In-vitro propagation of asiatic hybrid lily[J].Indian Journal of Agricultural Sciences,2002,72(11):639-642.
[41]蔡奇英,杨柏云,连勇,等.马铃薯休眠阶段中内源激素变化的研究[J].中国马铃薯,2004,18(6):321-325.
[42]张伟,郭志刚.切花生产技术丛书——球根类[M].北京:清华大学出版社,1999.