高温对花花柴(Karelinia caspica)光系统Ⅱ的影响

doi:10.7522/j.issn.1000-694X.2013.00201

摘要/Abstract

摘要：

生长在沙漠地区的植物经常遭受夏季正午短时间的高温胁迫,这在很大程度上限制了荒漠植物的光合作用。为了研究不同温度处理对塔克拉玛干沙漠南缘本土植物花花柴（Karelinia caspica）光系统Ⅱ(PSⅡ)的影响,分别在35、38、42、45、48、52 ℃和55 ℃下胁迫15 min后,测定其叶绿素荧光诱导曲线。结果表明:（1）叶片温度超过42 ℃后,PSⅡ最大光化学效率（Fv/Fm）、单位面积上反应中心的数量（RC/CSo）、叶功能指数（PIABS）等PSⅡ荧光指标分别出现明显的升高或降低。（2）在45 ℃时,Fv/Fm、RC/CSo、PIABS等PSⅡ荧光指标出现大幅的升高或降低。（3）大于52 ℃时各指数出现极值。因此,42 ℃为花花柴正常生长的热阈值,超过此温度,会对花花柴的生长造成抑制;45 ℃为花花柴的PSⅡ的结构和功能受到伤害的阈值;52 ℃为失活的阈值,超过52 ℃,花花柴的PSⅡ反应中心将发生不可逆失活。

关键词: 光系统Ⅱ, 叶绿素荧光, 高温胁迫, 花花柴（Karelinia caspica）, 塔克拉玛干沙漠

Abstract:

To assess effect of high temperature on photosynthesis of Karelinia caspica, an experiment was conducted at the southern fringe of the Taklimakan desert. We investigated the chlorophyll a fluorescence induction kinetics underlying heat-induced inhibition of photosystem II (PSⅡ) of K. caspica after 15 min heat stress treatment. The result indicated that the maximum fluorescence (Fm), the maximum photochemical efficiency of PSⅡ (Fv/Fm), the number of active reaction centers per cross-section (RC/CSo), performance index on absorption basis (PIABS) and chlorophyll fluorescence transients changed little at 35-42 ℃, and drastically at 45-55 ℃. Moreover, at 45 ℃ showed a clear “K” step in the chlorophyll fluorescence transients of K. caspica, and become inactivity at 55 ℃. We conclude that the temperature sensitivity point of K. caspica is 45 ℃ and inactivity point is 52 ℃.

Key words: photosystem II, chlorophyll fluorescence transient, heat stress, Karelinia caspica, Taklamakan desert

中图分类号:

Q945.79

陈凤丽1,2, 靳正忠1, 李生宇1, 徐新文1, 李磊1,2,3. 高温对花花柴(Karelinia caspica)光系统Ⅱ的影响[J]. 中国沙漠, 2013, 33(5): 1371-1376.

CHEN Feng-li1,2, JIN Zheng-zhong1, LI Sheng-yu1, XU Xin-wen1, LI Lei1,2,3. Effects of Heat Stress on Photosystem II in Karelinia caspica[J]. JOURNAL OF DESERT RESEARCH, 2013, 33(5): 1371-1376.

参考文献

[1]Walther G R,Post E,Convey P,et al. Ecological responses to recent climate change[J].Nature,2002,416(6879):389-395.

[2]Briantais J M,Dacosta J,Goulas Y,et al.Heat stress induces in leaves an increase of the minimum level of chlorophyll fluorescence,Fo:a time-resolved analysis[J].Photosynthesis Research,1996,48(1):189-196.

[3]Srivastava A,Guiss B,Greppin H,et al.Regulation of antenna structure and electron transport in photosystem II of Pisum sativum under elevated temperature probed by the fast polyphasic chlorophyll a fluorescence transient:OKJIP [J].Biochimica et Biophysica Acta (BBA)-Bioenergetics,1997,1320(1):95-106.

[4]Crafts-Brandner S J,Salvucci M E.Sensitivity of photosynthesis in a C4 plant,maize,to heat stress [J].Plant Physiology,2002,129(4):1773-1780.

[5]Sharkey T D.Effects of moderate heat stress on photosynthesis:importance of thylakoid reactions,rubisco deactivation,reactive oxygen species,and thermotolerance provided by isoprene[J].Plant,Cell & Environment,2005,28(3):269-277.

[6]Lu C M,Zhang J H.Heat-induced multiple effects on PSⅡ in wheat plants[J].Journal of Plant Physiology,1999,156:259-265.

[7]Fryer M J,Andrews J R,Oxborough K,et al.Relationship between CO2 assimilation,photosynthetic electron transport,and active O2 metabolism in leaves of maize in the field during periods of low temperature[J].Plant Physiology,1998,116(2):571-580.

[8]李鹏民,高辉远,Strasser R J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理学与分子生物学学报,2005,31(6):559-566.

[9]Jiang C D,Gao H Y,Zou Q.Changes of donor and accepter side in photosystem II complex induced by iron deficiency in attached soybean and maize leaves[J].Photosynthetia,2003,41:267-271.

[10]Baker N R.A possible role of photosystem II in environmental perturbations of photosynthesis[J].Physiologia Plantarum,1991,81:563-570.

[11]Havaux M.Short-term responses of photosystem I to heat stress:induction of PSⅡ-independent electron transport through PS I fed by stromal components[J].Photosynthesis Research,1996,47:85-97.

[12]宋春雨,刘晓冰,金彩霞.高温胁迫下光合器官受损及其适应机理[J].农业系统科学与综合研究,2002,18(4):252-256.

[13]Musil C F,Van-Heerden P D R,Cilliers C D,et al.Mild experimental climate warming induces metabolic impairment and massive mortalities in southern African quartz field succulents[J].Environmental and Experimental Botany,2009,66:79-87.

[14]Bruelheide H,Jandt U,Gries D,et al.Vegetation changes in a river oasis on the southern rim of the Taklamakan Desert in China between 1956 and 2000 [J].Phytocoenologia,2003,33:801-818.

[15]Appenroth K J,Stckel J,Srivastava A,et al.Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements[J].Environmental Pollution,2001,115:49-64.

[16]刘明,孙世贤,齐华,等.水分胁迫对玉米苗期叶绿素荧光参数的影响[J].玉米科学,2009,17(3):95-98.

[17]孙宪芝,郑成淑,王秀峰.高温胁迫对切花菊‘神马’光合作用与叶绿素荧光的影响[J].应用生态学报,2008,19(10):2149-2154.

[18]刘鹏,康华靖,张志详,等.香果树幼苗生长特性和叶绿素荧光对不同光强的响应[J].生态学报,2008,28(11):5656-5664.

[19]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.

[20]Schreiber U,Berry J A.Heat induced changes in chlorophyll fluorescence in intact leaves correlated with damage of the photosynthetic apparatus[J].Planta,1977,136:233-238.

[21]Sundby C,Melis A,Menp P,et al.Temperature-dependent changes in the antenna size of photosystem II:reversible conversion of photosystem IIα to photosystem IIβ [J].Biochimica et Biophysica Acta (BBA)-Bioenergetics,1986,851:475-483.

[22]温国胜,田海涛,张明如,等.叶绿素荧光分析技术在林木培育中的应用[J].应用生态学报,2006,17(10):1973-1977.

[23]李磊,李向义,林丽莎,等.两种生境条件下6种牧草叶绿素含量及荧光参数的比较[J].植物生态学报,2011,35(6):672-680.

[24]Strasser R J,Srivastava A,Govindjee.Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria[J].Photochemistry Photobiology,1995,61:32-42.

[25]张静,张元明.冻融过程对生物结皮中齿肋赤藓叶绿素荧光特性的影响[J].中国沙漠,2011,31(6):1479-1487.

[26]张亚娟,谢忠奎,赵学勇,等.水分胁迫对东方百合光合特性、叶绿素荧光参数及干物质积累的影响[J].中国沙漠,2011,31(4):884-888

[27]韩张雄,李利,徐新文,等.两种盐生植物幼苗在NaCl处理下对昼夜温差变化的叶绿素荧光响应[J].中国沙漠,2010,30(1):63-68.

[28]韩张雄,李利,徐新文,等.梭梭幼苗干物质积累和叶绿素荧光对NaCl胁迫的响应[J].中国沙漠,2011,31(1):90-95.

[29]Van-Heerden P D R,Tsimilli-Michael M,Krüger G H J,et al.Dark chilling effects on soybean genotypes during vegetative development:parallel studies of CO2 assimilation,chlorophyll a fluorescence kinetics O-J-I-P and nitrogen fixation[J].Physiology Plant,2003,117:476-491.

[30]Chen L S,Li P M,Chen L L,et al.Effects of high temperature coupled with high light on the balance between photooxidation and photoprotection in the sun-exposed peel of apple[J].Planta,2008,228(5):745-756.

[31]Strasser R J,Tsimill-Michael M,Srivastava A.Analysis of the chlorophyll a fluorescence transient[M]//Papageorgiou G,Govindjee.Advances in Photosynthesis and Respiration.Netherlands:KAP Press,2004:1-47.

[32]Strivastava A,Strasser R J.Stress and stress management of land plants during a regular day[J].Journal of Plant Physiology,1996,148:445-455.

[33]薛伟,李向义,林丽莎,等.短时间热胁迫对疏叶骆驼刺光系统II、Rubisco活性和活性氧化剂的影响[J].植物生态学报,2011,35(4):441-451.