关键词: 锦鸡儿, RAPD, 遗传多样性, 种间关系

Abstract: RAPD markers were used to assess the genetic diversity of Caragana Fabr. and to estimate genetic relationships among the eleven species growing in Gansu province. The 11 primers were screened from 34 10-bp arbitrary primers, and a total of 128 loci were amplified; the length of fragments was between 200 bp and 2 000 bp, among which, 104(81.25) were polymorphic. The Shannon's information index and Nei s gene diversity were 0.3889 and 0.2517, respectively. The results showed that the Caragana Fabr. had a high level genetic diversity, and its adaptability to environmental change was strong. A dendrogram based on genetic distances was constructed by UPGMA method of POPGENE version 1.32. The conclusion was almost consistent with the results of traditional morphologic taxa, except hypotaxis status of very few species was slightly changed. It indicated that it is necessary for further studying their relative relations to combine molecular markers with morphologic and other methods.

Key words: Caragana Fabr., RAPD, genetic diversity, genetic relationship