不同海拔区兰州百合(Lilium davidii.var.unicolor)栽培群体遗传多样性

doi:10.7522/j.issn.1000-694X.2016.00063

摘要/Abstract

摘要： 为了研究不同海拔区兰州百合（Lilium davidii.var.unicolor）栽培群体的遗传多样性和遗传分化，应用ISSR技术，用8条ISSR引物对甘肃七道梁4个海拔区兰州百合栽培群体共175个个体进行扩增，共扩增出59条带，其中多态性条带为51条。结果显示：（1）兰州百合栽培群体在物种水平遗传多样性较高，物种水平上PPF=86.44%，h=0.2163，I=0.3413；（2）兰州百合栽培群体间遗传相似很高，4个栽培群体间的遗传一致度0.9860~0.9951；遗传分化系数均值Gst=0.113，栽培群体间变异占总变异的11.3%，群体内变异占总变异的88.7%，基于Gst的判定结果表明，各栽培群体遗传分化中等；（3）基于Gst值估算基因流Nm=3.9247，选择以及迁移是影响各栽培群体间Nm，群体遗传平衡，种群间遗传结构分化的主要因素。

关键词: 兰州百合(Lilium davidii.var.unicolor), ISSR, 遗传多样性, 海拔

Abstract: To investigate the genetic diversity of Lanzhou Lily in different altitude regions. nter simple sequence repeat(ISSR) molecular technique was used to evaluate the genetic diversity and population structure with 8 primers for 175 from 4 populations at different altitudes at Qidao Liang Hills. Fifty one polymorphic loci were detected from total 59 loc. ISSR analysis results showed that: (1) Genetic diversity at the species level is higher, and the PPF was 86.44%, h is 0.2163, and I is 0.3413. (2) The cultivated populations demonstrated relatively high genetic identity with similarity coefficient was 0.9860-0.9951; The variation between and within populations was accounted for 11.3% of total variance, respectively. The Gst value was 0.113, which indicated genetic differentiation level of populations was medium. (3) The mean of gene flow (Nm) was 3.9247 based on the Gst value estimation. Selection and migration were the primary reasons that affected genetic equilibrium of populations, gene flow (Nm), and population genetic structure between different populations.

Key words: Lilium davidii.var.unicolor, ISSR, genetic diversity, altitude

中图分类号:

Q311

李谋强, 陈君良, 黄彦玮, 边小荣, 叶树辉, 师桂英. 不同海拔区兰州百合(Lilium davidii.var.unicolor)栽培群体遗传多样性[J]. 中国沙漠, 2016, 36(4): 1029-1033.

Li Mouqiang, Chen Junliang, Huang Yanwei, Bian Xiaorong, Ye Shuhui, Shi Guiying. Genetic Diversity of Cultivated Populations of Lilium davidii.var.unicolor at Different Altitudes[J]. JOURNAL OF DESERT RESEARCH, 2016, 36(4): 1029-1033.

参考文献

[1] 徐学军,梁玉文,胡相莉.百合新品种兰州百合1号[J].中国蔬菜,2010(13):30-31.
[2] 徐学军,梁玉文,刘凯.百合新品种兰州百合2号[J].中国蔬菜,2010(20):34.
[3] 张彦妮,钱灿种.百合属植物花粉形态扫描电镜观察[J].草业学报,2011,20(5):111-118.
[4] 崔光芬,吴丽芳,吴学尉.基于IS序列分析豹子花属与5种百合的亲缘关系[J].西北植物学报,2008,28(10):1941-1946.
[5] 陈艳华,史宝秀,谢玲.甘肃中部百合气候适应性及适生种植区划[J].甘肃气象,2003,24(3):51-53.
[6] 陈艳华,郭俊琴,张旭东.基于GIS的兰州百合适生种植气候区划[J].干旱气象,2014,32(1):157-161.
[7] 李谋强,边小荣,黄彦玮,等.兰州百合ISSR-PCR体系的优化[J].甘肃农业大学学报,2014,49(6):76-81.
[8] 李恩香,黄玉琴,蒋满英.江西省龙牙百合种质资源遗传多样性研究[J].园艺学报2010,37(5):811-816.
[9] 李晶.山丹栽培群体和部分百合群体遗传多样性的ISSR分析[D].河北保定:河北农业大学,2012.
[10] 邓明文.岷江百合种质资源遗传多样性研究[D].南京:南京林业大学,2008.
[11] Hamrick J L,Godt M J W.Effects of life history traits on genetic diversity in plant species[J].Philosophical Transactions of the Royal,1996.DOI:10.1098/rstb.1996.0112.
[12] 丁永电,于凯然. 花食两用龙牙百合微繁技术研究[J].江西园艺,2005,6:45.
[13] 王丽艳,荆瑞勇.兰州百合与川百合试管苗结鳞茎研究[J].中国农村小康科技,2008(12):46-47.
[14] 李佳.中药远志遗传多样性的ISSR分析[D].西安:西北大学,2010.
[15] Wen C S,Hsiao J Y.Genetic differentiation of Lilium longiflorum Thunb.var.scabrum Masam.(Liliaceae) in Taiwan using Random Amplified Polymorphic DNA and morphological characters[J].Botanical Bulletin of Academia Sinica,1999,40(1):65-71.
[16] Wright S.The genetical structure of populations[J].Annals of Eugenics,1951,15(4):323-54.
[17] Wright S.Evolution in Mendelian populations[J].Genetics,1931,16(2):97-159.
[18] 林玉红,郭凤霞,罗俊杰.不同施氮水平对旱地兰州百合养分累积与氮肥利用的影响[J].草业学报,2011,20(2):101-108.
[19] 徐学军,梁玉文,唐大为.叶面追肥对兰州百合植株生长及鳞茎产量的影响[J].长江蔬菜,2011(10):59-61.