在从北京怀柔风沙防治技术示范区采集的沙样中，采用96孔板有限稀释法分离出的微生物，形态学初步鉴定分别为蓝细菌。进行PCR和测序后，利用GenBank中蓝细菌不同科、属种类序列进行分子系统分析、系统发育树及遗传距离分析，结果表明测序样品应为Microcoleus vaginatus和Microcoleus streenstrupii。
In this paper, kinds of sand samples were isolated from Huairou, Beijing, using the method of 96-well plate limited dilution,and preliminarily identified as Synechococcus based on their morphological features respectively. ITS regions of these three samples was amplified, cloned to sequenced and finally blasted against the GenBank to compare with the homolog genes from the different genera in Synechococcus. The construction of phylogenetic tree and genetic distance analysis suggested the three samples may belong to Microcoleus vaginatus or Microcoleus streenstrupii.
[1] West N E.Structure and function of microphytic soil crusts in wildland ecosystem of arid to semi-arid regions[J].Advance in ecological research,1990,20:179-223.
[2] Eldridge D J,Greene R S B.Microbiotic soil crusts:a view of their roles in soil and ecological processes in the rangelands of Australia[J].Australian Journal of Soil Research,1994,32:389-415.
[3] Belnap J,Lange O L.Biological Soil Crusts:Structure,Functio,and Management[M].Berlin,Germany:Springer-Verlag,2001:137-169.
[4] 李新荣,张元明,赵允格.生物土壤结皮研究:进展、前沿与展望[J].地球科学进展,2009,24(1):11-24.
[5] Gilad E.Ecosystem engineers:from pattern formation to habitat creation[J].Physical Review Letters,2004,93:125-137.
[6] Byers J E.Using ecosystem engineers to restore ecological systems[J].Trends in Ecology and Evolution,2006,21:493-500.
[7] Li X R,Wang X P,Li T,et al.Microbiotic soil crust and its effect on vegetation and habit on artificially stabilized desert dunes in Tengger desert,North China[J].Biology and Fertility of Soils,2002,35:147-154.
[8] Patrick E.Researching crusting soils:themes,trends,recent developments and implications for managing soil and water resources in dry areas[J].Progress in Physical Geography,2002,26:442-461.
[9] Zhang Y M.The spatial distribution patterns of biological soil crusts in the Gurbantunggut Desert,Northem Xinjiang,China[J].Journal of Arid Environments,2007,68:599-610.
[10] Williams J D,Dobrowolski J P,Gillette D A,et al.The role of microphytic crusts on wind induced erosion[C].Proceeding 46th Annual Meeting Society of Range Management,Albuquerque,New Mexico,USA,1993:255-254.
[11] Hu C,Liu Y,Paulsen B Smestad,et al.Extracellular carbohydrate polymers from five desert soil algae with different cohesion in the stabilization of five sand grain[J].Carbohydrate Polymers,2003,54:33-42.
[12] Garcia-Pichel F,Johnson S.L,Youngkin D,et a1.Small-scale vertical distribution of bacterial biomass and diversity in biological soil crusts from Arid Lands in the Colorado Plateau[J].Microbial Ecology,2003,46:312-321.
[13] Li X R,Xiao H L,He M Z.Sand barriers of straw checkerboards for habitant restoration in extremely arid desert regions[J].Ecological Engineering,2006,28:149-157.
[14] Kranner I,Beckett R P,Varma A K.Protocols in Lichenology,Culturing,Biochemistry,Ecophysiology and Use in Biomonitoring[M].Berlin,Heidelberg,Germany:Springer-Verlag,2002:580-585.
[15] Itenman I,Rippka R,Tandeau de Marsac N,et a1.Comparison of conserved structural and regulatory domains within divergent 16S rRNA 16S rRNA-23S rRNA spacer sequences of cyanobacteria[J].Microbiology,2000,146:1275-1286.
[16] 朱浩然.中国淡水藻志(第九卷)[M].北京:科学出版社,2007:74-77.
[17] Boyer S L,Johansen V R,Flechtner G L.Phylogeny and genetic variance in terrestrial Microcoleus (Cyanophyceae) species based on sequence analysis of the 16S rRNA gene and associated 16S-23S ITS region[J].Journal of Phycology,2002,38:1222-1235.
甘公网安备 62010202000688号