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生物与土壤

LED补光对温室黄瓜幼苗抗衰老及抗氧化酶系统的影响

  • 闫晓花 ,
  • 郁继华
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  • 甘肃农业大学 园艺学院, 甘肃 兰州 730070
闫晓花(1979-),女,甘肃兰州人,博士研究生,主要从事设施蔬菜栽培生理研究。E-mail:gsauyxh@126.com

收稿日期: 2015-11-22

  修回日期: 2016-01-13

  网络出版日期: 2016-03-20

基金资助

国家现代农业产业技术体系建设资金项目(CARS-25-C-07)

Effects of Supplemental LED Light on Photosynthetic Pigment Contents and Antioxidant Enzyme Activities of Cucumber Seedling Leaves

  • Yan Xiaohua ,
  • Yu Jihua
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  • College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2015-11-22

  Revised date: 2016-01-13

  Online published: 2016-03-20

摘要

研究补光时间与光质对设施作物抗衰老及抗氧化能力的影响,对于充实光质效应理论和优化补光技术有重要意义。研究了补光2、4、8 h及蓝光(B)、红光(R)、白光(W)和红蓝复合光(R:B=7:3和8:2)对温室黄瓜幼苗光合色素、丙二醛(MDA)、可溶性蛋白含量及抗氧化能力的影响。结果表明:与自然光照相比,补光可显著提高黄瓜幼苗叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,其中以补光4 h红蓝复合光处理(7R/3B和8R/2B)效果最为显著;补光可提高黄瓜幼苗可溶性蛋白含量,其中,以蓝光效果最好。补照4 h蓝光可以明显提高黄瓜幼苗叶片可溶性蛋白的含量及保护酶(过氧化物酶POD、超氧化物歧化酶SOD、抗坏血酸过氧化物酶APX、过氧化氢酶CAT)活性,并且使MDA的含量保持相对较低的水平,从而可以有效延缓叶片衰老。

本文引用格式

闫晓花 , 郁继华 . LED补光对温室黄瓜幼苗抗衰老及抗氧化酶系统的影响[J]. 中国沙漠, 2016 , 36(2) : 392 -398 . DOI: 10.7522/j.issn.1000-694X.2016.00005

Abstract

This study explored the effects of supplemental light quality and illumination duration on photosynthetic pigment contents and antioxidant enzyme activities of greenhouse cucumber seedling leaves. Three lighting durations (2, 4, and 8 h) of supplemental lighting applied were combined with five light qualities: (i) 100% White light (W), (ii) 100% Red light (R), (iii) 100% Blue light (B), (iv) a 3:7 mixture of B and R, (v) a 2:8 mixture of B and R. The effects of white, red, blue, red and blue mixed light on photosynthetic pigment contents, MDA contents, soluble proteins contents and antioxidant enzyme activities of greenhouse cucumber seedling leaves were studied. The results showed that, compared with natural light, supplemental lighting could significantly improve the chlorophyll a, chlorophyll b, chlorophyll content and carotenoid content of cucumber seedling, and the effect of combined light components (7R/3B and 8R/2B) for the duration of 4 h were the most significant. And that supplemental lighting could significantly improve the content of soluble protein,blue light had the greatest the content of soluble protein. Meanwhile, supplemental blue lighting for 4 h promoted significantly the contents of soluble protein and the activities of anti-oxidant enzymes (POD, SOD, APX and CAT). In contrast, supplemental blue lighting for 4 h decreased the contents of MDA. These results indicate that supplemental blue lighting for 4 h prevent the senescence by maintaining high levels of anti-oxidant enzymes.

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