LED补光对温室黄瓜幼苗抗衰老及抗氧化酶系统的影响

doi:10.7522/j.issn.1000-694X.2016.00005

摘要/Abstract

摘要： 研究补光时间与光质对设施作物抗衰老及抗氧化能力的影响,对于充实光质效应理论和优化补光技术有重要意义。研究了补光2、4、8 h及蓝光(B)、红光(R)、白光(W)和红蓝复合光(R:B＝7:3和8:2)对温室黄瓜幼苗光合色素、丙二醛(MDA)、可溶性蛋白含量及抗氧化能力的影响。结果表明:与自然光照相比,补光可显著提高黄瓜幼苗叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,其中以补光4 h红蓝复合光处理(7R/3B和8R/2B)效果最为显著;补光可提高黄瓜幼苗可溶性蛋白含量,其中,以蓝光效果最好。补照4 h蓝光可以明显提高黄瓜幼苗叶片可溶性蛋白的含量及保护酶(过氧化物酶POD、超氧化物歧化酶SOD、抗坏血酸过氧化物酶APX、过氧化氢酶CAT)活性,并且使MDA的含量保持相对较低的水平,从而可以有效延缓叶片衰老。

关键词: 发光二极管(LED), 光质, 光合色素, 可溶性蛋白, 抗氧化酶

Abstract: This study explored the effects of supplemental light quality and illumination duration on photosynthetic pigment contents and antioxidant enzyme activities of greenhouse cucumber seedling leaves. Three lighting durations (2, 4, and 8 h) of supplemental lighting applied were combined with five light qualities: (i) 100% White light (W), (ii) 100% Red light (R), (iii) 100% Blue light (B), (iv) a 3:7 mixture of B and R, (v) a 2:8 mixture of B and R. The effects of white, red, blue, red and blue mixed light on photosynthetic pigment contents, MDA contents, soluble proteins contents and antioxidant enzyme activities of greenhouse cucumber seedling leaves were studied. The results showed that, compared with natural light, supplemental lighting could significantly improve the chlorophyll a, chlorophyll b, chlorophyll content and carotenoid content of cucumber seedling, and the effect of combined light components (7R/3B and 8R/2B) for the duration of 4 h were the most significant. And that supplemental lighting could significantly improve the content of soluble protein,blue light had the greatest the content of soluble protein. Meanwhile, supplemental blue lighting for 4 h promoted significantly the contents of soluble protein and the activities of anti-oxidant enzymes (POD, SOD, APX and CAT). In contrast, supplemental blue lighting for 4 h decreased the contents of MDA. These results indicate that supplemental blue lighting for 4 h prevent the senescence by maintaining high levels of anti-oxidant enzymes.

Key words: light emitting diode (LED), light quality, photosynthetic pigment, soluble protein content, antioxidant enzyme

中图分类号:

Q945.79

闫晓花, 郁继华. LED补光对温室黄瓜幼苗抗衰老及抗氧化酶系统的影响[J]. 中国沙漠, 2016, 36(2): 392-398.

Yan Xiaohua, Yu Jihua. Effects of Supplemental LED Light on Photosynthetic Pigment Contents and Antioxidant Enzyme Activities of Cucumber Seedling Leaves[J]. JOURNAL OF DESERT RESEARCH, 2016, 36(2): 392-398.

参考文献

[1] 李伟,眭晓蕾,张振贤.温度对黄瓜幼苗光合生理弱光耐受性的影响[J].应用生态学报,2008,19(12):2643-2650.
[2] 唐凤鸾,黄宁珍,黄志民,等.自然光照下补照不同光质光对马蹄莲光合速率及生长的影响[J].植物生理学通讯,2007,43(5):879-881.
[3] Lin K H,Huang M Y,Huang W D,et al.The effects of red, blue, and white light-emitting diodes on the growth,development, and edible quality of hydroponically grown lettuce (Lactuca sativa L.var.capitata)[J].Scientia Horticulturae,2013,150:86-91.
[4] 鲁燕舞,张晓燕,耿殿祥,等.光质对萝卜芽苗菜总酚类物质含量及抗氧化能力的影响[J].园艺学报,2014,41(3):545-552.
[5] Samuolien G,Sirtautas R,BrazaitytA,et al.LED lighting and seasonality effects antioxidant properties of baby leaf lettuce[J].Food Chemistry,2012,134:1494-1499.
[6] 王永健,张海英,张峰,等.低温弱光对不同黄瓜品种幼苗光合作用的影响[J].园艺学报,2001,28(3):230-234.
[7] 周艳虹,喻景权,钱琼秋,等.低温弱光对黄瓜幼苗生长及抗氧化酶活性的影响[J].应用生态学报,2003,14(6):921-924.
[8] 李向东,王晓云,张高英,等.花生叶片衰老过程中某些酶活性的变化[J].植物生理学报,2001,27(4):353-358.
[9] 孙玉莹,毕京翠,赵志超,等.作物叶片衰老研究进展[J].作物杂志,2013(4):11-19.
[10] Xu F,Shi L Y,Chen W,et al.Effect of blue light treatment on fruit quality,antioxidant enzymesand radical-scavenging activity in strawberry fruit[J].Scientia Horticulturae,2014,175:181-186.
[11] 文锦芬,柯学,徐超华,等.光质对烟草叶片生长发育过程中抗氧化系统的影响[J].西北植物学报,2011,31(9):1799-1804.
[12] Wu M C,Hou C Y,Jiang C M,et al.A novel approach of LED light radiation improves the antioxidant activity of pea seedlings[J].Food Chemistry,2007,101:1753-1758.
[13] Sæbø A,Krekling T,Appelgren M.Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro[J].Plant Cell,Tissue and Organ Culture,1995,41(2):177-185.
[14] Lichtenthaler H K.Applications of Chlorophyll Fluorescene.Photosynthesis Research,Stress Physiology,Hydrobiology and Remote Sensing[M].Dordrecht,Netherlands:Kluwer Academic Publishers,1988:129-142.
[15] Hodges D M,Delong J M,Forney C F,et al.Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds[J].Planta,1999,207:604-611.
[16] Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72:248-254.
[17] 王虹,姜玉萍,师恺,等.光质对黄瓜叶片衰老与抗氧化酶系统的影响[J].中国农业科学,2010,43(3):529-534.
[18] Amako K,Chen G X,Asada K.Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolicisozymes of ascorbate peroxidase in plants[J].Plant Cell Physiology,1994,35(3):497-504.
[19] Giannopolitis C N,Ries S K.Superoxide dismutases:I.occurrence in higher plants[J].Plant Physiology,1977,59:309-314.
[20] Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J].Plant and Cell Physiology,1981,22:867-880.
[21] Patra H K,Kar M,Mishra D.Catalase activity in leaves and cotyledons during plant development and senescence[J].Biochemie und Physiologie der Pflanzen,1978,172:385-390.
[22] 郑洁,胡美君,郭延平.光质对植物光合作用的调控及其机理[J].应用生态学报,2008,19(7):1619-1624.
[23] 杨淑慎,高俊凤,李学俊.高等植物叶片的衰老[J].西北植物学报,2001,21(6):223-229.
[24] 闫萌萌,王铭伦,王洪波,等.光质对花生幼苗叶片光合色素含量及光合特性的影响[J].应用生态学报,2014,25(2):483-487.
[25] 徐凯,郭延平,张上隆.不同光质对草莓叶片光合作用和叶绿素荧光的影响[J].中国农业科学,2005,38(2):369-375.
[26] 刘庆,连海峰,刘世琦,等.不同光质LED光源对草莓光合特性、产量及品质的影响[J].应用生态学报,2015,26(6):1743-1750.
[27] 艾希珍,马兴庄,于立明,等.弱光下长期亚适温和短期低温对黄瓜生长及光合作用的影响[J].应用生态学报,2004,15(11):2091-2094.
[28] 林碧英,张瑜,林义章.不同光质对豇豆幼苗光合特性和若干生理生化指标的影响[J].热带作物学报,2011,32(2):235-239.
[29] 郝俊江,陈向东,兰进.光质对灵芝生长及抗氧化酶系统的影响[J].中草药,2011,42(12):2529-2534.
[30] 周艳虹,喻景权,钱琼秋,等.低温弱光对黄瓜幼苗生长及抗氧化酶活性的影响[J].应用生态学报,2003,14(6):921-924.
[31] 郁继华,张国斌,冯致,等.低温弱光对辣椒幼苗抗氧化酶活性与质膜透性的影响[J].西北植物学报,2005,25(12):2478-2483.
[32] 杜建芳,廖祥儒,叶步青,等.光质对油菜幼苗生长及抗氧化酶活性的影响[J].植物学通报,2002,19(6):743-745.
[33] Causin Humberto F,Jauregui Rosa N,Barneix Atilio J.The effect of light spectral quality on leaf senescence and oxidative stress in wheat[J].Plant Science,2006,171(1):24-33.
[34] 王洪涛,艾希珍,郑楠,等.嫁接对低温弱光下辣椒幼苗膜脂过氧化及抗氧化酶活性的影响[J].应用生态学报,2010,21(5):1289-1294.