盐胁迫对枸杞(Lycium barbarum)叶片生理的影响

doi:10.7522/j.issn.1000-694X.2017.00107

摘要/Abstract

摘要： 枸杞（Lycium barbarum）耐盐碱能力强，在盐渍化土壤中种植枸杞具有一定程度改良土壤的功效。然而对枸杞在不同盐渍生境和不同盐渍化程度下的生理适应基础研究较少。选取扦插的"宁杞5号"一年实生苗为材料，分别在渗透势为-0.48、-0.82、-1.18 MPa下采用单盐（NaCl或Na₂SO₄）和混合盐NaCl+Na₂SO₄进行处理，对比分析不同处理时段内枸杞生理活性的响应。结果表明：随着渗透势降低，叶绿素（Chl）含量呈下降趋势；代表膜脂过氧化程度的丙二醛（MDA）、渗透调节物质脯氨酸（Pro）和可溶性糖（SS）含量明显增加；抗氧化酶系统超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性增加；渗透势为-1.18 MPa盐处理则抑制了过氧化物酶（POD）的活性。相同渗透势的不同盐处理之间，Chl、Pro、MDA和SS含量以及SOD和CAT活性在混合盐处理下或多或少都有增加；随着处理时间的延长，MDA、Pro、SS含量和CAT活性增加。这说明盐渍化程度增加，渗透调节物质和抗氧化酶系统的保护加强；不同盐渍环境下枸杞的生理响应不同，-1.18 MPa下混合盐胁迫相对于单盐影响更大；胁迫时间延长，渗透调节物质的累积增加，枸杞的适应性增强。

关键词: 枸杞, 单盐, 混合盐, 渗透调节物质, 抗氧化酶系统

Abstract: Lycium barbarum has strong ability to salt and alkali resistance, and its cultivation in salinized soil can improve soil quality to a certain extent. However, less information was found about physiological adaptation to different saline habitats under different salinization level in L. barbarum. In order to analyze the physiological response difference of L. barbarum to single salt (NaCl or Na₂SO₄) and mixed salt (NaCl+Na₂SO₄), we selected one year seedling of Ningqi No.5 as the materials to treatment under different saline solution with different osmotic potential gradients (-0.48, -0.82 and -1.18 MPa, respectively). The results showed that chlorophyll (Chl) content decreased, but the content of malondialdehyde (MDA), proline (Pro) and soluble sugar (SS), and activities of anti-oxidant enzyme superoxide dismutase (SOD) and catalase (CAT) increased with the decreasing of osmotic potential under different salt treatment. Peroxidase (POD) activity was inhibited at osmotic potential of -1.18 MPa. Among different salt solution with the same osmotic potential, the contents of Chl, Pro, MDA and SS and activities of SOD and CAT more or less increased under mixed salt treatment. MDA, Pro and SS contents and CAT activity increased during treatment. All these results suggested that the protective capability enhanced from osmo-regulated substances and anti-oxidant enzyme system of L. barbarum at high salinization level. The physiological responses of L. barbarum were different in different saline environments, and the effect of mixed salt stress was greater than that of single salt at -1.18 MPa. The accumulation of osmo-regulated substances and ability to saline adaptation of L. barbarum increased during salt treatment.

Key words: Lycium barbarum, single salt, mixed salt, osmo-regulated substances, anti-oxidant enzyme system

中图分类号:

Q945.78

张雯莉, 刘玉冰, 刘立超. 盐胁迫对枸杞(Lycium barbarum)叶片生理的影响[J]. 中国沙漠, 2018, 38(2): 294-299.

Zhang Wenli, Liu Yubing, Liu Lichao. Effect of Single and Mixed Salt Stress on Leaf Physiological Characteristics of Lycium barbarum[J]. JOURNAL OF DESERT RESEARCH, 2018, 38(2): 294-299.

参考文献

[1] Shabala S.Learning from halophytes:physiological basis and strategies to improve abiotic stress tolerance in crops[J].Annals of Botany,2013,112:1209-1221.
[2] Britto D T,Kronzucker H J.Sodium efflux in plant roots:what do we really know?[J].Journal of Plant Physiology,2015,186/187:1-12.
[3] Shi D C,Yin L J.Difference between salt (NaCl) and alkaline (Na₂CO₃) stresses on Puccinellia tenuiflora (Griseb.) Scribn.etMerr.Plants[J].Acta Botany Sinica,1993,35:144-149.
[4] Shi D C,Wang D L.Effects of various salt alkali mixed stresses on Aneurolepidium chinense (Trin.) Kitag[J].Plant and Soil,2005,271:15-26.
[5] Reginato M,Sosa L,Llanes A,et al.Na₂SO₄ and NaCl determine different growth responses and ion accumulation in the halophytic legume Prosopis strombulifera[J].Plant Biology,2014,16:97-106.
[6] Pagter M,Bragato C,Malagoli M,et al.Osmotic and ionic effects of NaCl and Na₂SO₄ salinity on Phragmites australis[J].Aquatic Botany,2009,90:43-51.
[7] Bie Z,Tadashi I,Shinoara Y.Effects of sodium sulfate and sodium bicarbonate on the growth,gas exchange and mineral composition of lettuce[J].Scientia Horticulturae,2004,99:215-224.
[8] Manivannan P,Abdul Jaleel C,Sankar B,et al.Mineral uptake and biochemical changes in Helianthus annuus under treatment with different sodium salts[J].Colloids and Surfaces B:Biointerfaces,2008,62:58-63.
[9] Tarchoune I,Sgherri C,Izzo R,et al.Antioxidative responses of Ocimum basilicum to sodium chloride or sodium sulphate salinization[J].Plant Physiology and Biochemistry,2010,48:772-777.
[10] Reginato M,Travaglia C,Reinoso H,et al.Salt mixtures induce anatomical modifications in the halophyte Prosopis strombulifera (Fabaceae:Mimosoideae)[J].Flora,2016,218:75-85.
[11] 樊丽琴,杨建国,许兴,等.宁夏引黄灌区盐碱地土壤盐分特征及相关性[J].中国农学通报,2012,28(35):221-225.
[12] Wei Y,Xu X,Tao H,et al.Growth performance and physiological response in the halophyte Lycium barbarum grown at salt-affected soil[J].Annals of Applied Biology,2006,149:263-269.
[13] 钟鉎元.枸杞高产栽培技术[M].北京:金盾出版社,2012.
[14] 王培根.宁夏中宁县枸杞产业竞争力研究[D].陕西杨凌:西北农林科技大学,2013.
[15] 王恩军,李善家,韩多红,等.中性盐和碱性盐胁迫对黑果枸杞种子萌发及幼苗生长的影响[J].干旱地区农业研究,2014,32(6):64-69.
[16] Qi Y,Wang J,Wang Q,et al.Physiological response of Chinese wolfberry seedling to NaCl stress[J].Agricultural Science & Technology,2016,17(6):1301-1304.
[17] 张立军,樊金娟.植物生理学实验教程[M].北京:中国农业大学出版社,2007.
[18] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002.
[19] 李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[20] 李子银,张劲松,陈受宜.水稻盐胁迫应答基因的克隆、表达及染色体定位[J].中国科学C辑:生命科学,1999,29(6):561-570.
[21] 罗君,彭飞,王涛,等.黑果枸杞(Lycium ruthenicum)种子萌发及幼苗生长对盐胁迫的响应[J].中国沙漠,2017,37(2):261-267.
[22] 刘玉冰,李新荣,谭会娟,等.荒漠地区两种生态型芦苇叶片的抗氧化生理特性分析[J].中国沙漠,2011,31(2):277-281.
[23] Reginato M A,Castagna A,Furlan A,et al.Physiological responses of a halophytic shrub to salt stress by Na₂SO₄ and NaCl:oxidative damage and the role of polyphenols in antioxidant protection[J].AoB Plants,2016,6(4):367-372.
[24] Foowers T J,Colmer T D.Salinity tolerance in halophytes[J].New Phytologist,2008,179(4):945-963.
[25] Koyro H W,Hussain T,Huchzermeter B,et al.Photosynthetic and growth responses of a perennial halophytic grass Panicum turgidum to increasing NaCl concentrations[J].Environmental & Experimental Botany,2013,91(91):22-29.
[26] 谢敏,回嵘,刘立超,等.沙漠生物土壤结皮中真藓(Bryum argenteum)和土生对齿藓(Didymodon vinealis)对降雪的生理响应[J].中国沙漠,2017,37(1):116-123.