海岸和内陆沙地砂引草(Messerschmidia sibirica)对自然环境和沙埋处理适应的生理差异

doi:10.7522/j.issn.1000-694X.2015.00109

摘要/Abstract

摘要： 砂引草(Messerschmidia sibirica)是一种耐盐、耐旱固沙地被植物,既能生长在海岸沙地并成为优势植物,也在内陆干旱沙地有分布。本文以科尔沁内陆沙地和烟台海岸沙地生长的砂引草为试验材料,研究了两沙地土壤化学特性,水温特性及在自然状况和沙埋胁迫条件下两沙地砂引草叶片丙二醛(MDA)含量、抗氧化酶活力和渗透调节物含量的变化特征。结果表明:(1)海岸沙地土壤含盐量较内陆沙地高5倍,但土壤地表温度略低。(2)在自然和沙埋条件下,两沙地砂引草叶中MDA含量较低。(3)生活在内陆干旱高温沙地上的砂引草,在自然和沙埋处理中叶片平均脯氨酸和可溶性糖含量均高于海岸沙地砂引草,自然状况下,其叶片平均脯氨酸和可溶性糖含量分别较海岸沙地的高25%~50%和高3.5倍。(4)海岸沙地砂引草在自然和沙埋处理5 d和10 d中,其叶片平均超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活力均大于内陆沙地砂引草,且两者差异显著(p<0.05),自然状况下海岸沙地砂引草叶片SOD、POD、CAT平均活力分别较内陆高14%、140%和118%。(4)两沙地砂引草对沙埋胁迫的生理响应类似,随着沙埋强度的增加砂引草沙上叶片MDA含量变化不大,SOD和CAT活力和脯氨酸含量增加,可溶性糖含量下降。研究表明,砂引草具有在不同环境下利用不同渗透调节剂维护水分代谢平衡、提高抗氧化酶活力抑制膜脂过氧化维持氧自由基代谢平衡的生理调控能力,该生理可塑性可能是其具有较宽生态幅的生理基础。

关键词: 砂引草(Messerschmidia sibirica), 海岸沙地, 内陆沙地, 沙埋, 抗氧化酶活力, 渗透调节物

Abstract: Messerschmidia sibirica is a ground cover plant with higher resistance to salt, drought and sand-fixation, and is a dominant plant in coastal sand land(CSL) and also grow in inland sand land(ISL). In this paper, M.sibirica grown in Horqin Sandy Land(ISL) and coastal sand land in Yantai were used as material. The soil chemical properties and temperature and water content in both two sand lands, and malondialdehyde(MDA) content, the activities of antioxidative enzyme and osmoregulation substance contents in leaves of M.sibirica were measured in nature environment(NE) and under sand burial(SB). The results showed that:(1) The soil in CSL had higher in Na⁺ content by 5 times than that of ISL, but had lower surface temperature than ISL.(2)The leaves of M.sibirica from both sand lands kept lower MDA content in NE and under SB.(3)The leaves of M.sibirica grown in ISL with drought and higher temperature had higher contents in proline and soluble sugar than that of CSL in NE and under SB. For instance, in NE, the contents of proline and soluble sugar in leaves of M.sibirica from ISL were higher by 25%-50%, and 3.5 times than that from CSL.(4)The leaves of M.sibirica grown in CSL had higher activities of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) than that of ISL in NE and under SB for 5 days and 10 days, for instance, in NE, it had higher in the activities of SOD, POD, CAT by 14%, 140% and 118% than that from ISL.(4)Both M.sibirica grown in ISL and CSL had same response to SB. The content of proline and activities of SOD and CAT increased, and content of soluble sugar decreased in the leaves above sand with increasing of sand burial depth. It suggest that M.sibirica had a higher ability of physiological regulation by changing osmotic regulator in maintaining water metabolism balance, and increasing activities of antioxidative enzyme to inhibition of lipid peroxidation, which would be foundation of physiological plasticity for it to have a wider ecological amplitude.

Key words: Messerschmidia sibirica, coastal sand land, inland sand land, sand burial, antioxidant enzymes, osmotic regulation substances

中图分类号:

Q945.79

解卫海, 周瑞莲, 梁慧敏, 曲浩, 董龙伟, 强生斌. 海岸和内陆沙地砂引草(Messerschmidia sibirica)对自然环境和沙埋处理适应的生理差异[J]. 中国沙漠, 2015, 35(6): 1538-1548.

Xie Weihai, Zhou Ruilian, Liang Huimin, Qu Hao, Dong Longwei, Qiang Shengbin. Physiological Difference in Messerschmidia sibirica Grown in Inland and Coastal Sand Land in Nature Environment and under Sand Burial[J]. JOURNAL OF DESERT RESEARCH, 2015, 35(6): 1538-1548.

参考文献

[1] 王涛,赵哈林,肖洪浪.中国沙漠化研究的进展[J].中国沙漠,1999,19:299-311.
[2] 李志文,李宝生,王丰年.海岸沙丘发育机制之研究现状评述[J].中国沙漠,2011,31:357-366.
[3] 许崇彦,刘宪斌,刘占广.翘碱蓬对石油烃污染的海岸带修复的初步研究[J].安全与环境学报,2007,7:37-39.
[4] 赵哈林,曲浩,周瑞莲,等.沙埋对差巴嘎蒿幼苗存活、生长及光合特性的影响[J].中国草地学报,2014,2:6-11.
[5] 赵哈林,曲浩,周瑞莲,等.沙埋对沙米幼苗生长及生理过程的影响[J].应用生态学报,2013,24:3367-3372.
[6] 何玉惠,赵哈林,赵学勇,等.沙埋对小叶锦鸡儿幼苗生长和生物量分配的影响[J].干旱区地理,2008,31:701-706.
[7] 原鹏飞,丁国栋,赵奎.流动沙丘沙埋对沙柳生长特性的影响[J].水土保持研究,2008,15:44-47.
[8] 周瑞莲,王进,杨淑琴,等.海滨沙滩单叶蔓荆对沙埋的生理响应特征[J].生态学报,2013,33:1973-1981.
[9] 周瑞莲,杨树德,左进城,等.海滨沙地单叶蔓荆匍匐茎对沙埋适应生长对策[J].生态学报,2015,35:7-15.
[10] 王进,周瑞莲,赵哈林,等.海滨沙地砂引草对沙埋的生长和生理适应对策[J].生态学报,2012,32:4291-4299.
[11] Bradshaw A D.Evolutionary significance of phenotypic plasticity in plants[J].Advanced Genetics,1965,13:116-155.
[12] Atkin O K,Loveys B R,Atkinson L J.Phenotypic plasticity and growth temperature:understanding interspecific variability[J].Journal of Experimental Botany,2006,57:267-281.
[13] 项秀丽,初庆刚,刘振乾.砂引草泌盐腺的结构与泌盐的关系[J].暨南大学学报(自然科学版),2008,29:305-310.
[14] 张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.
[15] Drazkiewicz M,Skórzyńska-Polit E,Krupa Z.Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana[J].BioMetals,2004,17:379-387.
[16] 张治安,陈展宇.植物生理学实验技术[M].长春:吉林大学出版社,2008.
[17] 章加恩.生态学常用实验研究方法与技术[M].北京:化学工业出版社,2006.
[18] 中国科学院南京土壤研究所.土壤理化分析[M].上海:上海科技出版社,1978.
[19] 林大仪.土壤学实验指导[M].北京:中国林业出版社,2004.
[20] Zhang J,Kirkham M B.Enzymatic responses of the ascorbate-tlutathion cycle to drought in sorghum and sunflower plants[J].Plant Science,1996,113:139-148.
[21] Smirnoff N.The role of active oxygen in the response of plants to water deficit and desiccation[J].New Phytologist,1993,125:27-35.
[22] Gossett D R,Millhollon E P,Lucas M C.Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton[J].Crop Science,1994,34:706-716.
[23] Hern ndez J A,Corpas F J,Gomez M,et al.Salt-induced oxidative stresses mediated by activated oxygen species in pea leaf mitochondria[J].Plant Physiology,1993,89:103-108.
[24] Chaves M M,Maroco J P,Pereira J S.Understanding plant responses to drought from genes to the whole plant[J].Functional Plant Biology,2003,30:239-246.
[25] Bor M,Ozdemir F,Turkan I.The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar been Beta vulgaris L.and wild beet Beta maritima L[J].Plant Science,2003,164:77-84.
[26] Luna C,Garcia-Seffino L,Atias C,et al.Oxidative stress indicators as selection tools for salt tolerance[J].Plant Breeding,2000,119:341-345.
[27] Xiong L,Zhu JK.Molecular and genetic aspects of plant responses to osmotic stress[J].Plant,Cell and Environment,2002,25:131-139.
[28] 赵可夫,范海.盐生植物及其对盐渍生境的适应生理[M].北京:科学出版社,2005:238-240.
[29] Mansour M M F,Salama K H A.Cellular basis of salinity tolerance in plants[J].Environmental and Experimental Botany,2004,52:113-122.
[30] Pan Y,Wu L J,Yu Z L.Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice(Glycyrrhiza uralensis Fisch)[J].Plant Growth Regulation,2006,49:157-165.