矿业废弃地先锋植物盐生草在镍、铜处理下抗氧化酶系统的变化

doi:10.7522/j.issn.1000-694X.2013.00017

摘要/Abstract

摘要：

采用温室盆栽法，研究镍(Ni)、铜(Cu)单一处理（0、50、100、200、400 mg·kg-1）对金昌Ni/Cu尾矿库先锋植物盐生草(Halogeton glomeratus)幼苗Ni、Cu积累、膜脂过氧化程度与抗氧化酶系统的影响。结果表明：盐生草幼苗叶片及根部Ni、Cu含量随土壤中Ni、Cu含量的增加而增加，Ni、Cu含量以根部含量为高；叶片及根部丙二醛(MDA)含量随Ni、Cu处理浓度升高显著增加，膜脂过氧化程度加剧；Ni胁迫下盐生草叶片中仅抗坏血酸酶(APX)活性随Ni浓度增加总体呈增加趋势，在对抗Ni胁迫引起的氧化胁迫方面发挥着主要作用，根部超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、APX和谷胱甘肽还原酶(GR)活性随Ni浓度增加呈上升趋势，在减轻Ni胁迫引起的氧化损伤方面起着重要作用；Cu胁迫下盐生草叶片SOD和APX活性较对照有所增强，在减弱Cu胁迫引起的氧化损伤方面发挥着一定作用，根部上述5种抗氧化酶活性随Cu浓度增加总体呈增加趋势，在缓解Cu胁迫引起的氧化损伤方面发挥着重要作用。

关键词: 镍, 铜, 盐生草, 抗氧化酶, 膜脂过氧化

Abstract:

Halogeton glomeratus seedlings planted in plastic pots in a greenhouse were treated separately with NiSO4·5H2O and CuSO4·6H2O solutions at 0, 50, 100, 200, and 400 mg·kg-1 concentration levels (computed with single Ni or Cu element). Impacts of the treatments on Ni, Cu accumulation, lipid peroxidation and antioxidative enzymes system in the roots and leaves of H. glomeratus were examined. The results showed that: ① The Ni, Cu contents in the leaves and roots of H. glomeratus both increased in a dose-dependent manner, and more in roots; ② The Malondialdehyde (MDA) contents significantly increased in leaves and roots of H. glomeratus with increasing Ni and Cu concentrations, indicating a lipid peroxidation aggravation; ③ In the leaves of H. glomeratus, only ascorbate peroxidase (APX) activity increased with increasing Ni concentrations, indicating that APX could contribute to protection against Ni induced oxidative stress. While in the roots of H. glomeratus, the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), APX, and glutathione reductase (GR) enhanced with increasing Ni concentrations, suggesting that they played important roles in weakening Ni induced oxidative damages; ④ Under Cu stress, the activities of SOD and APX in leaves of H. glomeratus ascended compared with control level, and they could be effective in lessening Cu induced oxidative damage; ⑤ In the roots of H. glomeratus, as a whole, the five antioxidative enzymes activities increased with increasing Cu concentrations, thus might protect seedlings from Cu induced oxidative damage by exerting a certain antioxidant function.

Key words: Ni, Cu, Halogeton glomeratus, antioxidative enzymes, lipid peroxidation

中图分类号:

Q945

鲁艳1, 李新荣2, 何明珠2, 赵昕2, 李小军2, 王进2, 曾凡江1. 矿业废弃地先锋植物盐生草在镍、铜处理下抗氧化酶系统的变化[J]. 中国沙漠, 2013, 33(1): 118-125.

LU Yan1, LI Xin-rong2, HE Ming-zhu2, ZHAO Xin2, LI Xiao-jun2, WANG Jin2, ZENG Fan-jiang1. Impact of Single Ni and Cu Treatment on Activities of Antioxidative Enzymes of Halogeton glomeratus, A Pioneer Plant Growing in Ni/Cu Mining Wasteland[J]. JOURNAL OF DESERT RESEARCH, 2013, 33(1): 118-125.

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