古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性

韩彩霞; 张丙昌; 张元明; 邰凤姣; 张弛; 邵华

doi:10.7522/j.issn.1000-694X.2015.00063

中国沙漠 >

2016 , Vol. 36 >Issue 4: 1050 - 1055

DOI: https://doi.org/10.7522/j.issn.1000-694X.2015.00063

生物与土壤

古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性

韩彩霞 ,
张丙昌 ,
张元明 ,
邰凤姣 ,
张弛 ,
邵华

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1. 中国科学院新疆生态与地理研究所干旱区生物地理与生物资源重点实验室, 新疆乌鲁木齐 830011;
2. 中国科学院新疆生态与地理研究所荒漠与绿洲生态国家重点实验室, 新疆乌鲁木齐 830011;
3. 中国科学院大学, 北京 100049

韩彩霞(1980-),女,河南许昌人,硕士研究生,主要从事植物化学与植物资源研究.E-mail:hancaixia2007@sina.com

收稿日期: 2014-12-26

修回日期: 2015-04-08

网络出版日期: 2016-07-20

基金资助

国家基础科学研究计划项目（2014CB954204）；国家自然科学基金项目（U1203301，U1503103）

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Diversity of Culturable Fungi from the Moss Crusts Growing in the Southern Gurbantunggut Desert

Han Caixia ,
Zhang Bingchang ,
Zhang Yuanming ,
Tai Fengjiao ,
Zhang Chi ,
Shao Hua

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1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
2. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2014-12-26

Revised date: 2015-04-08

Online published: 2016-07-20

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摘要

为了解古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性，运用稀释平板法和基于ITS-rDNA基因序列的系统发育分析，将得到的基因序列与NCBI数据库GenBank中的序列进行相似性比较并构建系统发育树。结果显示，从古尔班通古特沙漠南缘苔藓结皮中共分离得到24株菌落形态各异的真菌，分属于11属21种，绝大多数属于子囊菌亚门和半知菌亚门。其中，青霉属（Penicillium）和链格孢属（Alternaria）为优势菌群，茎点霉属（Phoma）、曲霉菌属（Aspergillus）、镰刀孢菌属（Fusarium）为常见属；有些种类的真菌具有耐热及特殊产酶活性。

关键词： 古尔班通古特沙漠; 苔藓结皮; 可培养真菌; 多样性指数

本文引用格式

韩彩霞 , 张丙昌 , 张元明 , 邰凤姣 , 张弛 , 邵华 . 古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性[J]. 中国沙漠, 2016 , 36(4) : 1050 -1055 . DOI: 10.7522/j.issn.1000-694X.2015.00063

Abstract

The objective of this study was to evaluate the diversity of culturable fungi isolated from the moss crusts growing in the Southern Gurbantunggut Desert. Dilution plate technique and ITS-rDNA sequence analysis were applied toobtain the sequences of 24 different colonies isolated from the moss crusts, which were subsequently submitted to GenBank and compared with those known sequences to build up the Phylogenetic tree. Results revealed that these strains were clustered into 3 phyla, 7 classes, 8 orders, 10 families, 12 genera, and the majority of them belong to Ascomycotina and Deuteromycetes. The dominant genera were Penicillium and Alternaria, the other genera included Phoma, Aspergillus, Fusarium, ect. We also isolated a thermotolerant fungi MF12 (KJ935021) and a fungi that produces special enzyme MF11 (KJ935022), implying the potential of utilizing the moss crust resource in the Southern Gurbantunggut Desert.

Key words： Gurbantunggut Desert; moss crusts; culturable fungi; diversity index

参考文献

[1] West N E.Structure and function of microphytic soil crusts in wild land ecosystems of arid to semi-arid regions[J].Advances in Ecological Research,1990,20:179-223.
[2] Belnap J,Harper K T,Warren S D.Surface disturbance of cryptobiotic soil crusts:nitrogenase activity,chlorophyⅡcontent and chlorophyⅡ degradation[J].Arid Soil Research & Rehabilitation,1994,8:1-8.
[3] Smith M J,Walton D W H.A statistical analysis of the relationships among viable microbial populations,vegetation,and environment in a Subantarctic Tundra[J].Microbial Ecology,1985,11(3):245-257.
[4] Beringer J,Lynch A H,Chapin F S,et al.The representation of Arctic soils in the Land Surface Model (LSM):the importance of mosses[J].Journal of Climate,2001,14:3324-3335.
[5] 张丙昌,张元明,赵建成,等.古尔班通古特沙漠生物结皮不同发育阶段中藻类的变化[J].生态学报,2009,29(1):9-17.
[6] 张元明,潘惠霞,潘伯荣.新疆古尔班通古特沙漠生物土壤结皮对沙垄不同部位的选择性分布[J].水土保持学报,2004,18(4):61-64.
[7] 李新荣,张元明,赵允格.生物土壤结皮研究:进展、前沿与展望[J].地球科学进展,2009,24(1):11-26.
[8] Belnap J,Prasse R,Harper K T.Influence of biological soil crusts on soil environments and vascular plants[M]//Belnap J,Lange O L,eds.Biological Soil Crusts:Structure,Function and Management.Berlin,Germany:Springer-Verlag,2003:281-300.
[9] 郭轶瑞,赵哈林,赵学勇,等.科尔沁沙地人工林下结皮发育对表土特性影响的研究[J].中国沙漠,2007,21(6):1000-1006.
[10] 卜崇峰,杨建振,张兴昌.毛乌素沙地生物结皮层藓类植物培育试验研究[J].中国沙漠,2011,31(4):937-941.
[11] 陶冶,张元明,吴楠,等.生物结皮中齿肋赤藓叶片毛尖对植株含水量及水分散失的影响[J].干旱区地理,2011,34(5):800-808.
[12] 张元明,曹同,潘伯荣.新疆古尔班通古特沙漠南缘土壤结皮中苔藓植物的研究[J].西北植物学报,2002,22(1):18-23.
[13] 和克俭,曹鑫,黄晓霞,等.新疆古尔班通古特地区的土壤结皮及其保护[J].云南地理环境研究,2008,20(3):42-47.
[14] Jeffries D L,Link S O,Klopatek J M.CO₂ fluxes of cryptogamic crusts.I.response to resaturation[J].New Phytologist,1993,125:163-174.
[15] Beymer R J,Klopatek J M.Potential contribution of carbon by microphytic crusts in Pinyon Juniper Woodlands[J].Arid Soil Research and Rehabilitation,1991,5:187-198.
[16] 鲍婧婷,王进,陈翠云.固沙植被区生物土壤结皮中蓝藻群落的多样性[J].中国沙漠,2015,35(6):1592-1598.
[17] 屈建军,李毅.一种生物土壤结皮中蓝细菌(Microcoleus spp.)的分子鉴定[J].中国沙漠,2014,34(2):358-362.
[18] 魏江春.沙漠生物地毯工程-干旱沙漠治理的新途径[J].干旱区研究,2005,22(3):287-288.
[19] Chen J,Zhang Y M,Tamura M,et al.A new index for mapping lichen2 dominated biological soil crust in desert area[J].Remote Sensing of Environment,2005,96:165-175.
[20] 孙立夫,张艳华,裴克全.一种高效提取真菌总DNA的方法[J].菌物学报,2009,28(2):299-302.
[21] Larkin M A,Blackshields G,Brown N P,et al.Clustal W and clustal X version 2.0[J].Bioinformatics,2007,23(21):2947-2948.
[22] Tamura K,Dudley J,Nei M,et al.MEGA4:Molecular evolutionary genetics analysis (MEGA) software version 4.0[J].Molecular Biology and Evolution,2007,24:1596-1599.
[23] 刘敏,李潞滨,杨凯,等.冷箭竹根际土壤中可培养细菌的多样性[J].生物多样性,2008,16(1):91-95.
[24] 庞雄飞,尤民生.昆虫群落生态学[M].北京:中国农业出版社,1996:77-103
[25] Samson R A,Yilmaz N,Houbraken J,et al.Phylogeny and nomenclature of the genus Talaromyces and taxa accommodated in Penicillium subgenus Biverticillium[J].Studies in Mycology,2011,70(1):159-183.
[26] 于淑玲.土壤真菌在生态系统中物质分解作用的研究进展[J].邢台学报,2006,21(4):125-128.
[27] 吴楠,潘伯荣,张元明.土壤微生物在生物结皮形成中的作用及生态学意义[J].干旱区研究,2004,12(4):444-450.
[28] 胡春香,刘永定,宋立荣,等.半荒漠藻结皮中藻类的种类组成及分布[J].应用生态学报,2000,11(1):61-65.
[29] Montemurro N,Visconti A."Alternaria metabolites-chemical and biological data," in Alternaria Biology,Plant Disease and Metabolites[M]//Chelkowski J and Visconti A,Amsterdam,Netherlands:Elsevier,1992:449-557.
[30] 李彧娜,石贵阳,王武,等.Rhizopus microsporus var.chinensis生淀粉糖化酶的分离纯化及酶学性质[J].应用及环境生物学报,2010,16(5):714-718.
[31] Cooney D G,Emerson R.Thermophilic Fungi.An Account of Their Biology,Activities and Classification[M].San Francisco,USA:W.H.Freeman & Co.,1964.
[32] 赵宇龙,张晓军,金一获,等.毛乌素沙漠生物土壤结皮真菌群落多样性分析[J].内蒙古农业大学学报,2011,32(2):170-175.
[33] 王岳坤,洪葵.红树林土壤细菌群落16S rDNA V3片段PCR产物的DGGE分析[J].微生物学报,2005,45(2):201-204.

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