中国沙漠 ›› 2016, Vol. 36 ›› Issue (4): 1050-1055.DOI: 10.7522/j.issn.1000-694X.2015.00063

• 生物与土壤 • 上一篇    下一篇


韩彩霞1, 张丙昌1, 张元明1, 邰凤姣1,3, 张弛2, 邵华1   

  1. 1. 中国科学院新疆生态与地理研究所 干旱区生物地理与生物资源重点实验室, 新疆 乌鲁木齐 830011;
    2. 中国科学院新疆生态与地理研究所 荒漠与绿洲生态国家重点实验室, 新疆 乌鲁木齐 830011;
    3. 中国科学院大学, 北京 100049
  • 收稿日期:2014-12-26 修回日期:2015-04-08 出版日期:2016-07-20 发布日期:2016-07-20
  • 通讯作者: 邵华
  • 作者简介:韩彩霞(1980-),女,河南许昌人,硕士研究生,主要从事植物化学与植物资源研究
  • 基金资助:

Diversity of Culturable Fungi from the Moss Crusts Growing in the Southern Gurbantunggut Desert

Han Caixia1, Zhang Bingchang1, Zhang Yuanming1, Tai Fengjiao1,3, Zhang Chi2, Shao Hua1   

  1. 1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
    2. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-12-26 Revised:2015-04-08 Online:2016-07-20 Published:2016-07-20

摘要: 为了解古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性,运用稀释平板法和基于ITS-rDNA基因序列的系统发育分析,将得到的基因序列与NCBI数据库GenBank中的序列进行相似性比较并构建系统发育树。结果显示,从古尔班通古特沙漠南缘苔藓结皮中共分离得到24株菌落形态各异的真菌,分属于11属21种,绝大多数属于子囊菌亚门和半知菌亚门。其中,青霉属(Penicillium)和链格孢属(Alternaria)为优势菌群,茎点霉属(Phoma)、曲霉菌属(Aspergillus)、镰刀孢菌属(Fusarium)为常见属;有些种类的真菌具有耐热及特殊产酶活性。

关键词: 古尔班通古特沙漠, 苔藓结皮, 可培养真菌, 多样性指数

Abstract: The objective of this study was to evaluate the diversity of culturable fungi isolated from the moss crusts growing in the Southern Gurbantunggut Desert. Dilution plate technique and ITS-rDNA sequence analysis were applied toobtain the sequences of 24 different colonies isolated from the moss crusts, which were subsequently submitted to GenBank and compared with those known sequences to build up the Phylogenetic tree. Results revealed that these strains were clustered into 3 phyla, 7 classes, 8 orders, 10 families, 12 genera, and the majority of them belong to Ascomycotina and Deuteromycetes. The dominant genera were Penicillium and Alternaria, the other genera included Phoma, Aspergillus, Fusarium, ect. We also isolated a thermotolerant fungi MF12 (KJ935021) and a fungi that produces special enzyme MF11 (KJ935022), implying the potential of utilizing the moss crust resource in the Southern Gurbantunggut Desert.

Key words: Gurbantunggut Desert, moss crusts, culturable fungi, diversity index