古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性

doi:10.7522/j.issn.1000-694X.2015.00063

摘要/Abstract

摘要： 为了解古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性，运用稀释平板法和基于ITS-rDNA基因序列的系统发育分析，将得到的基因序列与NCBI数据库GenBank中的序列进行相似性比较并构建系统发育树。结果显示，从古尔班通古特沙漠南缘苔藓结皮中共分离得到24株菌落形态各异的真菌，分属于11属21种，绝大多数属于子囊菌亚门和半知菌亚门。其中，青霉属（Penicillium）和链格孢属（Alternaria）为优势菌群，茎点霉属（Phoma）、曲霉菌属（Aspergillus）、镰刀孢菌属（Fusarium）为常见属；有些种类的真菌具有耐热及特殊产酶活性。

关键词: 古尔班通古特沙漠, 苔藓结皮, 可培养真菌, 多样性指数

Abstract: The objective of this study was to evaluate the diversity of culturable fungi isolated from the moss crusts growing in the Southern Gurbantunggut Desert. Dilution plate technique and ITS-rDNA sequence analysis were applied toobtain the sequences of 24 different colonies isolated from the moss crusts, which were subsequently submitted to GenBank and compared with those known sequences to build up the Phylogenetic tree. Results revealed that these strains were clustered into 3 phyla, 7 classes, 8 orders, 10 families, 12 genera, and the majority of them belong to Ascomycotina and Deuteromycetes. The dominant genera were Penicillium and Alternaria, the other genera included Phoma, Aspergillus, Fusarium, ect. We also isolated a thermotolerant fungi MF12 (KJ935021) and a fungi that produces special enzyme MF11 (KJ935022), implying the potential of utilizing the moss crust resource in the Southern Gurbantunggut Desert.

Key words: Gurbantunggut Desert, moss crusts, culturable fungi, diversity index

中图分类号:

S154.38

韩彩霞, 张丙昌, 张元明, 邰凤姣, 张弛, 邵华. 古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性[J]. 中国沙漠, 2016, 36(4): 1050-1055.

Han Caixia, Zhang Bingchang, Zhang Yuanming, Tai Fengjiao, Zhang Chi, Shao Hua. Diversity of Culturable Fungi from the Moss Crusts Growing in the Southern Gurbantunggut Desert[J]. JOURNAL OF DESERT RESEARCH, 2016, 36(4): 1050-1055.

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