Soil microbial biomass and soil enzyme activities are key indicators of soil function and indicators of soil recovery and environmental changes. The dynamic patterns of soil microbial biomass carbon and nitrogen and soil enzyme activities in different soil layers (0-5, 5-10 and 10-20 cm) at revegetated areas in the southeastern fringe of the Tengger Desert, respectively, were investigated by taking moving sand dune as control. The results showed that:The soil microbial biomass carbon, microbial biomass nitrogen, urease, polyphenol oxidase, alkaline phosphatase, catalase, amylase, cellulase and sucrase all increased with the increasing restoration age, and decreased with the increasing soil depth, significant differences were found among different revegetated areas and different soil layers (P<0.05). And 0-5 cm soil layer changes most obviously. After 62-year-revegetation,the soil microbial biomass carbon, microbial biomass nitrogen, urease, polyphenol oxidase, alkaline phosphatase, catalase, amylase, cellulase and sucrase increased by 16.44, 8.79, 3.99, 3.01, 2.54, 19.35, 0.77, 0.65 and 16.61 times,and their average annual rate of change is 1.55, 0.21 mg·kg-1 and 6.14×10-4, 1.25×10-2, 9.32×10-4, 6.05×10-2, 8.22×10-5, 9.07×10-5, 4.24×10-3 mg·g-1·h-1 respectively. The soil microbial biomass carbon and nitrogen and soil enzyme activities were highly positive correlated with silt and clay content, pH, electronic conductivity, the concentrations of organic carbon, inorganic carbon, total nitrogen, available nitrogen, available phosphorus and available potassium, while negatively correlated with sand and bulk density. The results showed that planting xerophytic shrubs can effectively promote the restoration of sandy soil function and improve the environment in the sandy area.
[1] 李新荣,赵洋,回嵘,等.中国干旱区恢复生态学研究进展及趋势评述[J].地理科学进展,2014,33(11):1435-1443.
[2] Li X J,Yang H T,Shi W L,et al. Afforestation with xerophytic shrubs accelerates soil net nitrogen nitrification and mineralization in the Tengger Desert,Northern China[J].Catena,2018,169:11-20.
[3] Li X J,Li X R,Wang X P,et al.Changes in soil organic carbon fractions after afforestation with xerophytic shrubs in the Tengger Desert, Northern China[J].European Journal of Soil Science,2016,67(2):184-195.
[4] Li X J,Li X R,Song W,et al.Effects of crust and shrub patches on runoff,sedimentation,and related nutrient (C,N) redistribution in the desertified steppe zone of the Tengger Desert,Northern China[J].Geomorphology,2008,96(1):221-232.
[5] He N P,Wu L,Wang Y S,et al.Changes in carbon and nitrogen in soil particle-size fractions along a grassland restoration chronosequence in Northern China[J].Geoderma,2009,150(3):302-308.
[6] 李新荣,张志山,刘玉冰,等.中国沙区生态重建与恢复的生态水文学基础[M].北京:科学出版社,2016.
[7] Elsas J D V.Microbiological and biochemical properties[J].Scientia Horticulturae,1995,63(1):131-133.
[8] Quilchano C,Marañón T.Dehydrogenase activity in Mediterranean forest soils[J].Biology and Fertility of Soils,2002,35(2):102-107.
[9] 刘艳梅,杨航宇,李新荣.生物土壤结皮对荒漠区土壤微生物生物量的影响[J].土壤学报,2014,51(2):394-401.
[10] Acosta-martínez V,Cruz L,Sotomayor-ramírez D,et al.Enzyme activities as affected by soil properties and land use in a tropical watershed[J].Applied Soil Ecology,2007,35(1):35-45.
[11] Desserud P A,Naeth M A.Natural recovery of rough fescue (Festuca hallii (vasey) piper) grassland after disturbance by pipeline construction in central Alberta,Canada[J].Natural Areas Journal,2013,33(1):91-98.
[12] 李晓红.鄱阳湖湿地不同植物群落土壤养分和土壤酶活性垂直分布特征[J].水土保持研究,2019,26(1):69-75,81.
[13] Ge C,Xue D,Yao H.Microbial biomass,community diversity, and enzyme activities in response to urea application in tea orchard soils[J].Communications in Soil Science and Plant Analysis,2010,41(7):797-810.
[14] Cao C Y,Jiang D M,Teng X H,et al.Soil chemical and microbiological properties along a chronosequence of Caragana microphylla Lam.plantations in the Horqin sandy land of Northeast China[J].Applied Soil Ecology,2008,40(1):78-85.
[15] Zhang Y L,Chen L J,Chen X H,et al. Response of soil enzyme activity to long-term restoration of desertified land[J].Catena,2015,133:64-70.
[16] 石万里,王辉,马维伟.沙区植被恢复对土壤微生物量及活性的影响[J].中国沙漠,2017,37(3):507-513.
[17] Schlesinger W H,Pilmanis A M.Plant-soil interaction in deserts[J].Biogeochemistry,1998,42(1):169-187.
[18] 李新荣.干旱沙区土壤空间异质性变化对植被恢复的影响[J].中国科学,2005,35(4):361-370.
[19] Lan S B,Ouyang H L,Wu L,et al.Biological soil crust community types differ in photosynthetic pigment composition,fluorescence and carbon fixation in Shapotou region of China[J]. Applied Soil Ecology,2017,111:9-16.
[20] Zhang T,Jia R L,Yu L Y.Diversity and distribution of soil fungal communities associated with biological soil crusts in the southeastern Tengger Desert (China) as revealed by 454 pyrosequencing[J]. Fungal Ecology,2016,23:156-163.
[21] 关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
[22] Blank R R.Amidohydrolase activity,soil N status,and the invasive crucifer Lepidium latifolium[J]. Plant and Soil,2002,239(1):155-163.
[23] Su Y Z,L Y L,C J Y,et al.Influences of continuous grazing and livestock exclusion on soil properties in a degraded sandy grassland,Inner Mongolia,northern China[J].Catena,2005,59(3):270-278.
[24] Li J,Tong X,Awasthi M K,et al.Dynamics of soil microbial biomass and enzyme activities along a chronosequence of desertified land revegetation[J].Ecological Engineering,2018,111:22-30.
[25] Brockett B F,Prescott C E,Grayston S J.Soil moisture is the major factor influencing microbial community structure and enzyme activities across seven biogeoclimatic zones in Western Canada[J]. Soil Biology and Biochemistry,2012,44(1):9-20.
[26] An S S, Huang Y M, Zheng F L. Evaluation of soil microbial indices along a revegetation chronosequence in grassland soils on the Loess Plateau,Northwest China[J].Applied Soil Ecology,2009,41(3):286-292.
[27] 杨万勤,王开运.森林土壤酶的研究进展[J].林业科学,2004,40(2):152-159.
[28] Li X R,Xiao H L,Zhang J G,et al.Long-term ecosystem effects of sand-binding vegetation in the Tengger Desert, Northern China[J].Restoration Ecology,2004,12(3):376-390.
[29] Liu Y M,Yang H Y,Li X R,et al.Effects of biological soil crusts on soil enzyme activities in revegetated areas of the Tengger Desert,China[J].Applied Soil Ecology,2014,80:6-14.
[30] Enowashu E,Poll C,Lamersdorf N,et al.Microbial biomass and enzyme activities under reduced nitrogen deposition in a spruce forest soil[J].Applied Soil Ecology,2009,43(1):11-21.
[31] Li X R.Influence of variation of soil spatial heterogeneity on vegetation restoration[J].Science in China,2005,48(11):2020.
[32] Petr B,Josef T,Jan F,et al.Enzyme activities and microbial biomass in topsoil layer during spontaneous succession in spoil heaps after brown coal mining[J]. Soil Biology and Biochemistry,2008,40(9):2107-2115.