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| The Molecular Diversity of Endophytic Diazotrophic Bacteria from Three Typical Desert Shrubs and PCR-RFLP Analysis of Their Nitrogenase Gene nifH |
| Xu Zhengjin1, Luo Ming1, Wang Weixia2, Wang Chunli3 |
1. Agronomy College, Xinjiang Agricultural University, Urumqi 830052, China;
2. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
3. College of Pratacultural Science and Environmental, Xinjiang Agricultural University, Urumqi 830052, China |
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Abstract Endophytic diazotrophic bacteria inhabiting special ecological niche can provide benefits to the host plants such as improving nutrient supply , enhancing tolerance to stresses and maintaining community stability, so the potential areas for its practical application need to be explored. In this study, the genetic diversity and phylogeny of one hundred and thirty seven endophytic diazotrophic bacteria strains which were isolated from three typical desert shrubs, Tamarix ramosissima, Lycium ruthenicum and Halostachys caspic in arid zone of Xinjiang were studied by the methods of 16SrDNA PCR-RFLP, 16SrDNA sequencing, BOXAIR-PCR fingerprinting, nifH PCR-RFLP.The isolates were clustered into nine genotypes based on the restriction digest patterns of 16SrDNA PCR-RFLP with the enzymes MspⅠ, HaeⅢ, HinfⅠ, HhaⅠ, respectively, and the representatives of each genotype were randomly chosen for the determination of 16SrDNA sequence. The phylogenetic analysis revealed that the isolates were affiliated to six genera, consisting of Rahnella, Pseudomonas, Pantoea, Bacillus, Stenotrophomonas, Shinella, and dominant isolates were related to Rahnella. In addition, one isolate XJ-F6 showed less affiliation with known taxa (<97% sequence similarity) and may represent a new species. The strains in Rahnella were divided into 12 genotypes with BOX-PCR genomic fingerprinting analysis, which indicated that the tested strains had a great diversity at genomic level. nifH PCR-RFLP analysis indicated that there were three genetypes among all of the strains, which revealed highly conservative characteristic of nitrogenase gene (nifH). There was a certain amount of diversity between different nitrogen-fixing microbial populations and strains. This research work enriched the diversity of endophytic diazotrophs,and establish a found for their potetential application and protaction.
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Received: 06 February 2013
Published: 20 March 2014
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Corresponding Authors:
罗明（Email：luomingxjau@yahoo.com.cn）
E-mail: luomingxjau@yahoo.com.cn
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2014, Vol. 34 
