Abstract: The present study used the method of magnetic beads enrichment to build enriched microsatellite sequence library and analyzed the characteristics of library through sequencing results.The results showed that 13 922 microsatellite sequences were obtained with 88% perfect, 8% imperfect and 2% compound repeat motifs.Of these microsatellite loci, five type repeat primitives were presented, including prominent 60.7% three base primitive with (GGT)n, (TGG) n and (ACC)n, and 38.6% two base primitive with (AC)n, (GT)n and (CA)n. And the repeat time was mainly between 5 and 10, and declined as exceeding 30 times. Total 62 pairs of efficient PCR primers and 12 pairs of microsatellite primers have been screened by designing the primers with the 100 random microsatellite sequence and PCR screening with templates in populations with distant genetic and physical distance. Here, we revealed that the method of magnetic beads enrichment was high efficient and feasible for the development of Reaumuria soongarica genome microsatellite sequence. The present investigation would provide informative support and efficient genetic molecular marker for the genetic evolution and germplasm resources protection of Reaumuria soongarica.

Key words: Reaumuria soongarica, microsatellite, magnetic beads, enriched library