Effect of Nitrogen and Phosphorous Application on Nitrogen Accumulation, Allocation and Nitrate-reductase Activity in Kentucky Bluegrass

doi:10.7522/j.issn.1000-694X.2019.00071

Journal of Desert Research ›› 2019, Vol. 39 ›› Issue (5): 222-231.DOI: 10.7522/j.issn.1000-694X.2019.00071

Effect of Nitrogen and Phosphorous Application on Nitrogen Accumulation, Allocation and Nitrate-reductase Activity in Kentucky Bluegrass

Zhang Haowei, Bai Xiaoming, Chen Hui, Fan Jinghui, Li Xiongliang, Lian Yahong, Sun Min

College of Grassland Science/MOA Key Laboratory of Grassland Ecosystem/Pratacultural Engineering Laboratory of Gansu Province, Gansu Agricultural University, Lanzhou 730070, China

Received:2019-07-06 Revised:2019-08-25 Online:2019-09-29 Published:2019-09-29

Abstract

Abstract: To investigate the effect of nitrogen and phosphorous application on nitrogen accumulation, allocation and nitrate-reductase activity in Kentucky bluegrass (Poa pratensis), two varieties were selected under pot culture experiments. An experiment with a randomized block design was conducted to determine the effects of different phosphorous (P₂O₅) application rates (5, 15 and 25 g·m^-2) and nitrogen application rates (5, 10 and 15 g·m^-2) on nitrogen accumulation, allocation and nitrate-reductase activity in Kentucky bluegrass. The results showed that aboveground nitrogen accumulation, underground nitrogen accumulation and nitrogen accumulation in whole plant increased as nitrogen application rate increased. For the Kentucky bluegrass from Tianzhu county, the aboveground nitrogen accumulation, underground nitrogen accumulation and nitrogen accumulation in whole plant as phosphorous application rate increased. For the Kentucky bluegrass from Yuzhong county, the aboveground nitrogen accumulation and nitrogen accumulation in whole plant increased, whereas underground nitrogen accumulation increased firstly, and then decreased as phosphorous application rate increased. The aboveground nitrogen allocation increased as nitrogen and phosphorous application rate increased, while The underground nitrogen allocation and nitrogen use efficiency decreased as nitrogen and phosphorous application rate increased. The nitrate-reductase activity in Kentucky bluegrass increased as nitrogen application rate increased, whereas increased firstly, and then decreased as phosphorous application rate increased. The nitrogen accumulation and nitrogen use efficiency in Kentucky bluegrass from Yuzhong county was higher than that in Kentucky bluegrass from Tianzhu county. Effects of nitrogen application on nitrogen accumulation, allocation and nitrate-reductase activity was distinctly higher than that of phosphorous application. A significant positive correlation was found between nitrate-reductase activity and nitrogen application rate (P<0.01), and between nitrate-reductase activity and nitrogen accumulation (P<0.05).

Key words: Kentucky bluegrass, nitrogen and phosphate fertilizer, nitrogen accumulation, allocation, nitrate-reductase activity

CLC Number:

Q945.79

Zhang Haowei, Bai Xiaoming, Chen Hui, Fan Jinghui, Li Xiongliang, Lian Yahong, Sun Min. Effect of Nitrogen and Phosphorous Application on Nitrogen Accumulation, Allocation and Nitrate-reductase Activity in Kentucky Bluegrass[J]. Journal of Desert Research, 2019, 39(5): 222-231.

References

[1] 孙吉雄.草坪地被植物原色图谱[M].北京:金盾出版社,2008:1-5.
[2] 孙吉雄.草坪学[M].北京:中国农业出版社,2015:74-75.
[3] 宋桂云,侯迷红,孙德智,等.氮肥施用对科尔沁地区粮饲兼用玉米氮素积累及氮效率的影响[J].中国土壤与肥料,2017(6):93-98.
[4] 胡国智,张炎,胡伟,等.施磷对棉花磷素吸收、利用和产量的影响[J].中国土壤与肥料,2010(4):27-31.
[5] 张志国.草坪营养与施肥[M].北京:中国农业出版社,2004:99-102.
[6] 李瑾,侯向阳,马文静,等.不同磷环境下种间关系对羊草和冷蒿生物量及氮磷吸收利用效率的影响[J].中国草地学报,2019,41(1):17-24.
[7] 边秀举,胡林,张福锁,等.不同施肥时期对草坪草生长及草坪质量的影响[J].草原与草坪,2002(1):22-26.
[8] 孙佳林,张炜成,郑海霞,等.水氮交互作用下草地早熟禾生长特性的变化[J].草地学报,2015,23(6):1226-1232.
[9] 陈磊,郝明德,戚龙海.长期施肥对黄土旱塬区土壤植物系统中氮、磷养分的影响[J].植物营养与肥料学报,2007,13(6):1006-1012.
[10] 李新旺,门明新,王树涛,等.长期施肥对华北平原潮土作物产量及农田养分平衡的影响[J].草业学报,2009,18(1):9-16.
[11] 高杰,李青风,汪灿,等.不同氮素水平对糯高粱物质生产及氮素利用特性的影响[J].作物杂志,2017(6):126-130.
[12] 谷佳林.不同缓控释氮肥在草坪生态体系中氮素损失评价研究[D].石家庄:河北农业大学,2013.
[13] 武继承,杨永辉,康永亮,等.氮磷配施对玉米生长和养分利用的影响[J].河南农业科学,2011,40(10):68-71.
[14] 王兵.氮肥用量和栽培模式对西北旱地冬小麦生长和养分利用的影响[D].陕西杨凌:西北农林科技大学,2004.
[15] 侯云鹏,杨建,李前,等.施氮对水稻产量、氮素利用及土壤无机氮积累的影响[J].土壤通报,2016,47(1):118-124.
[16] 陈润娟.11个野生草地早熟禾根茎扩展与碳氮代谢物的关系研究[D].兰州:甘肃农业大学,2017.
[17] 李朝英,郑路,卢立华,等.测定植物全氮的H₂SO₄-H₂O₂消煮法改进[J].中国农学通报,2014,30(6):159-162.
[18] Vitousek P M.Nutrient cycling and nutrient use efficiency[J].The American Naturalist,1982,119:553-572.
[19] 邹琦.植物生理生化实验指导[M].北京:中国农业出版社,2000.
[20] Caroline G B, Michael J,Emes R,et al. Purification and properties of nitrite reductasefrom roots of pea (Pisum sativum v.Meteor)[J].Planta,1988,175:334-340.
[21] 赵满兴,周建斌,杨绒,等.不同施氮量对旱地不同品种冬小麦氮素累积、运输和分配的影响[J].植物营养与肥料学报,2006(2):2143-2149.
[22] 张明锦,陈良华,张健,等.干旱胁迫和施肥对巨能草生物量及C、N、P积累与分配的影响[J].西北农林科技大学学报:自然科学版,2016,44(9):105-112.
[23] 田秀英,石孝均.不同施肥对稻麦养分吸收利用的影响[J].重庆师范学院学报:自然科学版,2003,20(2):44-47.
[24] 王荣辉,王朝辉,李生秀,等.施磷量对旱地小麦氮磷钾和干物质积累及产量的影响[J].干旱地区农业研究,2011,29(1):115-121.
[25] 韩烈保.运动场草坪[M].北京:中国农业出版社,2011:111-113.
[26] 姜丹,陈雅君,刘丹,等.光氮互作对草地早熟禾碳氮代谢的影响[J].中国草地,2005(6):51-55+74.
[27] 黄发松.优质晚籼中国香稻的特征特性与栽培要点[J].中国稻米,2002(3):17.
[28] 王玉琴.内外生理条件对小麦黄化幼苗叶片亚硝酸还原酶活力的影响[J].植物生理学通讯,1988(4):18-20.
[29] 王玉波,李彩凤,马凤鸣,等.氮磷钾对甜菜硝酸还原酶与亚硝酸还原酶的影响[J].核农学报,2012,26(5):803-808.

Effect of Nitrogen and Phosphorous Application on Nitrogen Accumulation, Allocation and Nitrate-reductase Activity in Kentucky Bluegrass

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments