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| Diversity of Soil Microbe at Different Sites in Shelterbelt of Moving Sand Area |
| JIN Zheng-zhong1,2, LEI Jia-qiang1,2, XU Xin-wen1,2, LI Sheng-yu1,2, FAN Jing-long1,2, PENG Hui-qing3 |
| 1.Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; 2.National Engineering Technology Research Center for Desert-Oasis Ecological Construction, Urumqi 830011, China; 3.Tarim Branch of Petro China Company Limited, Korla 841000, Xinjiang, China |
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Abstract Soil microbe is crucial to soil development in extreme arid area. Methods of PLFA, PCR-DGGE, and cultivating and counting of microbe were used to investigate soil microbial quantity, fatty acid and microbial DNA segment at different sites in shelterbelt of Tarim Desert Highway because microbial isolation and culture techniques are able to culture less than 1% of the microbes present in soil. Results showed that the soil microbial amount, diversity indices of fatty acid and DNA segment differed notably among the different site conditions (F<F0.05), and they were bigger on middle parts and base parts of dunes than on top parts of dunes and harden flat sands, and all of them were better than on moving sands; Bacteria were dominant in soil microbial community (>84%), actinomycetes were second, and fungi were the least (<0.05%); Vertical difference of the soil microbial diversity was insignificant at 0~35 cm of soil. Correlation analysis indicated that the trees grew better as soil microbial diversity index enhanced. Therefore, the construction of shelterbelt along the Tarim Desert Highway promoted soil biological activities development, but we should consider effect of site condition on construction and regeneration of shelterbelt for enhancing sand control efficiency and promoting sandy soil development.
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Received: 01 September 2010
Published: 20 November 2011
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| [1]李阜棣,胡正嘉.微生物学[M].北京:中国农业出版社,2000:233-237.[2]Torsvik V,Φvreas L.Microbial diversity and function in soil: From genes to ecosystems[J].Ecology and Industrial Microbiology,2002,5:240-245.[3]顾峰雪,文启凯,潘伯荣,等.塔克拉玛干沙漠腹地人工植被下土壤微生物的初步研究[J].生物多样性,2000,8(3):297-303.[4]周智彬,李培军.塔克拉玛干沙漠腹地人工绿地土壤中微生物的生态分布及其与土壤因子间的关系[J].应用生态学报,2003,14(8):1246-1250.[5]樊自立,徐海量,张青青.塔克拉玛干沙漠中的古代交通路线[J].中国沙漠,2009,29(5):815-819.[6]汪言在,伍永秋,苟思薇.塔克拉玛干沙漠中部地区两类半隐蔽格状沙障内部沉积粒度特征浅析[J].中国沙漠,2009,29(6):1056-1062.[7]中国科学院南京土壤研究所微生物室.土壤微生物研究法[M].北京:科学出版社,1985:85-176.[8]Robert P,Larkin C,Wayne H,et al.Effect of swine and dairy manure amendments on microbial communities in three soils as influenced by environmental conditions[J].Biology and Fertility of Soils,2006,43:51-61.[9]Nancy J R,Marye S,Richard P D,et al.Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil[J].Applied and Environmental Microbiology,2000,66:1668-1675.[10]Duan X J,Min H.Diversity of microbial genes in paddy soil stressed by cadmium using DGGE[J].Environmental Science,2004,25(5):122-126.[11]Xu X Y,Min H,Liu H,et al.Comparison of DNA extraction methods for PCR-DGGE analysis of bacterial communities in soil[J].Journal of Agricultural Biotechnology,2005,13(3):377-381.[12]Van Elsas J D,Mantynen V,Wolters A C.Soil DNA extraction and assessment of the fate of Mycobacterium chlorophenolicum strain PCP-1 in different soils by 16S ribosomal RNA gene sequence based most-probable-number PCR and immunofluorescence[J].Biology and Fertility of Soils,1997,24:188-195.[13]Gerard M,Ellen C D,Andre G U.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology,1993,59(3):695-700.[14]Muyzer G,De Waal E C,Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes coding for 16S rDNA[J].Amplified and Environmental Microbiology,1993,59:695-700.[15]Bassam B J,Caetano-Anolles G.Fast and sensitive sliver staining of DNA in polyacrylamide gels[J].Annual Biochemistry,1991,196:80-83.[16]Gillan D C,Speksnijder A G,Zwart G,et al.Genetic diversity of the biofilm covering Montacuta ferrugi-nosa (Mollusca,Bivalvia) as evaluated by denaturing gradient gel electrophoresis analysis and cloning of PCR amplified gene fragment coding for 16S rDNA[J].Applied and Environmental Microbiology,1998,64(9):3464-3472.[17]Muyzer G,Brinkhoff T,Nubel U,et al.Denaturing gradient gel electrophoresis (DGGE) in microbial ecology[J].Molecular Microbial Ecology Manual,1998,34(4):1-27.[18]张晶,张惠文,苏振成,等.长期有机污水灌溉对土壤固氮细菌种群的影响[J].农业环境科学学报,2007,26(2):662-666.[19]靳正忠,雷加强,徐新文,等.沙漠腹地人工绿地土壤微生物变异与土壤环境因子关系的研究[J].中国生态农业学报,2008,16(6):1358-1364.[20]赵瑾,张丽华,钟芳,等.兰州市郊干旱半干旱黄土丘陵区不同立地条件土壤微生物区系特征[J].中国沙漠,2010,30(5):1160-1165.[21]Ringelberg D B,Davis J D,Smith G A,et al.Validation of signature polarlipid fatty acid biomarkers for alkane-utilizing bacteria in soils and subsurface aquifer materials[J].FEMS Microbiology Ecology,1989,62:39-50.[22]马悦欣.Carola Holmstrom,Jeremy Webb,等.变性梯度凝胶电泳(DGGE)在微生物生态学中的应用[J].生态学报,2003,8:1561-1569.[23]Fritze H,Pietikainen J,Pennanen T.Distribution of microbial biomass and phospholipid fatty acids in podzol profiles under coniferous forest[J].European Journal of Soil Science,2000,51:565-573.[24]Hold G L,Smith E A,Rappe M S,et al.Characterization of bacterial communities associated with toxic and non-toxic dinoflagellates:Alexandrium spp.and Scrippsiella trochoidea[J].FEMS Microbiological Ecology,2001,37(2):161-173.[25]刘永俊,冯虎元.不同演替阶段人工柠条林丛枝菌根真菌分子多样性研究[J].中国沙漠,2009,29(6):1141-1147. |
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2011, Vol. 31 
