Rhizobacterial community structures of three Kalidium species in the Liangucheng National Nature Reserve， China

doi:10.7522/j.issn.1000-694X.2024.00174

Journal of Desert Research ›› 2025, Vol. 45 ›› Issue (1): 249-258.DOI: 10.7522/j.issn.1000-694X.2024.00174

Rhizobacterial community structures of three Kalidium species in the Liangucheng National Nature Reserve， China

Lüzhuo Zhou¹(), Zongqiang Chang²^,³, Yuxia Wu¹()

^1.College of Ecology，Lanzhou University，Lanzhou 730000，China
^2.Key Laboratory of Ecological Safety and Sustainable Development in Arid lands，Northwest Institute of Eco-Environment and Resources，Chinese Academy of Sciences，Lanzhou 730000，China
^3.Inner Mongolia Badain Jaran Desert Ecosystem Research Station，Alxa Right Banner 737300，Inner Mongolia，China

Received:2024-10-10 Revised:2024-12-11 Online:2025-01-20 Published:2025-01-13
Contact: Yuxia Wu

Abstract

Abstract:

The rhizosphere microbial community significantly influences the growth and adaptation of halophytic plants in saline-alkaline soils. In this study， we selected three species of the genus Kalidium—Kalidium gracile， Kalidium foliatum， and Kalidium sinicum—whose fragmented/localized distributions are found within the Minqin Liangu Cheng National Nature Reserve. Using high-throughput sequencing technology， we analyzed the composition and diversity of the rhizosphere soil bacterial communities associated with these three species and investigated their environmental driving factors. The results showed that the soils in the distribution areas of all three species were classified as saline-alkaline. The distribution areas of K. foliatum and K. gracile primarily contained sulfates and chlorides， while the soil in the distribution area of K. sinicum was predominantly chloride. The dominant phyla in the rhizosphere soil samples of all three species were Proteobacteria and Bacteroidota. Notably， the α-diversity index of the rhizosphere soil samples from K. gracile was significantly higher than those of the other two species. Additionally， the distribution of 11 key microbial biomarkers in the rhizosphere soils varied among the different species. A positive correlation was observed between the pH of the rhizosphere soil and bacterial diversity， while the abundance of Flavobacteriales exhibited a negative correlation with salt ion concentrations. The relative abundance of dominant bacteria in the rhizosphere soil communities across the three Kalidium species showed variation that was positively correlated with the total soil salinity and the concentrations of major salt ions. These findings suggest that changes in soil pH and ion concentrations are key factors contributing to the differences in the bacterial community structure of the rhizosphere soils associated with the three Kalidium species in the Minqin Liangu Cheng Nature Reserve， which， to some extent， influence the regional distribution of these halophytic plants.

Key words: Kalidium, rhizosphere bacteria, salt ions, salt tolerance

CLC Number:

S154.3

Lüzhuo Zhou, Zongqiang Chang, Yuxia Wu. Rhizobacterial community structures of three Kalidium species in the Liangucheng National Nature Reserve， China[J]. Journal of Desert Research, 2025, 45(1): 249-258.

Figures/Tables 5

Fig.1 Sampling locations for three species in Kalidium

Fig.2 Ion content of rhizospheric soil （A） and plant shoot （B） of three species in Kalidium

Fig.3 The core bacterial communities in the rhizosphere soil of three species in Kalidium. （A） Venn analysis of shared， unique bacterial；（B） The relative abundance of major microbiome （The top ten dominant species at phylum level）；（C） The relative abundance of major microbiome （The top ten dominant species at order level）；（D） PCoA analysis

Fig.4 Biomarkers of rhizosphere soil of three species in Kalidium

Fig.5 The effects of soil salt ions on the bacterial communities in the rhizosphere of three species in Kalidium and their potential driving factors. （A） The environmental driving factors of rhizobacterial community structure in the rhizosphere soil of three species in Kalidium based on Mantel test；（B） The environmental driving factors of the dominant bacterial species in the rhizosphere soil of three species in Kalidium based on Spearman's correlation test

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Rhizobacterial community structures of three Kalidium species in the Liangucheng National Nature Reserve， China

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