| 生物土壤与生态 |
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| Induction and Proliferation of Callus in Desert Plant Reaumuria soongorica |
| LIU Yu-bing; ZHAO Xin; TAN Hui-juan |
| Shapotou Desert Research & Experimental Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
Abstract: Leaf and young stem were used as the explant to study callus induction and proliferation in desert plant Reaumuria soongorica. The results indicated that callus was well induced from tender shoot tips on MS agar medium supplemented with 2\^0 mg\5L-1 2,4-D and 0\^2 mg\5L-1 6-BA, pH at 5\^8, and the induction rate reached as high as 100%. The induction rate was lower in leaf than that in stem. The optimal conditions for callus proliferation were on the MS agar medium with 1\^0 mg\5L-1 2,4-D and 0\^1 mg\5L-1 6-BA, and the callus kept their incompact structure on such medium. Callus on media with different NaCl levels was found that it grew quickly in 50mM NaCl, but it was died in higher than 300 mM NaCl. |
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Abstract expalnt; Reaumuria soongorica; callus
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Received: 01 January 1900
Published: 20 March 2008
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2008, Vol. 28 
