| 生物土壤与生态 |
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| Effects of NaCl Stress on Tonoplast H+-ATPase and H+-PPase Activities in Peganum harmala Seedlings |
LIU Jian-xin1; HU Hao-bin2; ZHAO Guo-lin1
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| 1.Department of Life Science, Longdong University, Qingyang 745000, Gansu, China; 2.College of Chemistry & Chemical Engineering, Longdong University, Qingyang 745000, Gansu, China |
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Abstract Effects of the different concentration of NaCl stress on Na+ and K+ contents, tonoplast H+-ATPase and H+-PPase activities in peganum harmala seedling leaves were studied. The results showed that with NaCl stress concentration increased, K+ content in roots and leaves was decreased, but Na+ content, Na+/K+ ratio, K+/Na+ selectivity, as well as tonoplast H+-ATPase and H+-PPase activities were all increased, the H+/substrate stoichiometry was significantly unchanged, the activities of the ATP-dependent proton pump and the Na+/H+ antiport in leaves were increased, the activities of PPi-dependent proton pump in leaves and ATP and PPi-dependent proton pump and Na+/H+ antiport of the tonolast vesicles in roots were all increased and then decreased with the increase of NaCl concentration. These results indicated that the activity of Na+/H+ antiport and proton pump driven by H+-ATPase and H+-PPase in tonoplast of P. harmala plays an important role in Na+ accumulation in vacuoles and salt tolerance.
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Received: 15 January 2007
Published: 20 March 2008
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[1]李得禄,王继和,李爱德,等.3种驼绒藜属植物种子萌发期耐盐性试验研究[J].中国沙漠,2006,26(6):1009-1014.
[2]陈年来,马国军,张玉鑫,等.甜瓜种子萌发和和幼苗生长对NaCl胁迫的响应[J].中国沙漠,2006,26(5):814-819.
[3]Nill X,Bressan R A,Hasegawa J M,et al.Ion homeostasis in NaCl stress environments [J].Plant Physiol,1995,109:335-342.
[4]Liu J P,Zhu J K.A Calcium sensor homolog required for plant salt tolerance [J].Science,1998,280:1943-1945.
[5]Serrano R.Structure and function of plasma membrane ATPase[J].Annu Rev Plant Physiol,1989,40:61-94.
[6]Luttge U,Ratajczak R.The physiology,biochemistry and molecular biology of the plant vacuolar ATPase[C]∥ Leigh R A,Sanders D(eds).Advances in Botanical Research(Vol 25).The Plant Vacuole.Oxford: Academic Press,1997:253-296.
[7]Ballesteros E,Blumwald E,Donaire J P,et al.Na+/H+ antiport activity in tonoplast vesicles isolated from sunflower roots by NaCl stress[J].Physiol Plant,1997,99:328-334.
[8]Low R,Rockel B,Kirsch M,et al.Early salt stress effects on the differential expression of vacuolar H+-ATPase genes in roots and leaves of Mesembryanthemum crystallinum[J].Plant Physiol,1996,110:259-265.
[9]章文华,陈沁,刘友良.NaCl胁迫下大麦根系液泡膜H+-ATPase活性与膜流动性的关系[J].植物学报,2002,44(3):292-296.
[10]王宝山,邹琦.NaCl胁迫对高粱根、叶鞘和叶片液泡膜ATP酶和焦磷酸酶活性的影响[J].植物生理学报,2000,26(3):181-188.
[11]马骥,王勋陵.中国荒漠地区骆驼蓬属植物种类与分布[J].中国沙漠,1998,18(2):131-136.
[12]付爱红,陈亚宁,李卫红,等.干旱、盐胁迫下的植物水势研究与进展[J].中国沙漠,2005,26(3):744-749.
[13]张小由,龚家栋,周茂先.额济纳三角洲土壤盐分特征分析[J].中国沙漠,2004,24(4):442-447.
[14]杨小平,潘启超,潘伟光.骆驼蓬总生物碱的抗肝癌活性[J].癌症,1991,10(6):463-465.
[15]翁群芳,钟国华,胡美英,等.骆驼蓬提取物对松材线虫的生物活性及生理效应[J].中国农业科学,2005,25(5):2014-2022.
[16]刘建新,赵国林,薛林贵.多裂骆驼蓬生物碱类物质抑菌杀虫活性研究[J].植物保护,2006,32(5):41-44.
[17]刘建新,赵国林,雷蕊霞.骆驼蓬提取物对玉米种子萌发和壮苗的影响[J].西北植物学报,2004,24(5):903-906.
[18]刘建新,胡浩斌,赵国林,等.多裂骆驼蓬中生物碱类物质对小麦种子萌发和幼苗生长的影响[J].植物生理学通讯,2006,42(2):213-216.
[19]马骥,王勋陵,李俊祯,等.骆驼蓬属叶表皮特征的研究[J].草业学报,1997,6(4):49-51.
[20]马骥,王勋陵,赵松岭.骆驼蓬属种子氨基酸组成及其系统学意义[J].中国沙漠,1998,18(4):399-403.
[21]王宝山,赵可夫.小麦叶片中Na、K提取方法的比较[J].植物生理学通讯,1995,31(1):50-52.
[22]Flowers T J,Yeo A R.Ion relations of salt tolerance[C]∥Baker D A,Hall J L.Solute Transport in Plant Cells and Tissues.New York:Jone Wiley & Sons,1988: 392-412.
[23]朱美君,陈珈,朱庆鸿,等.两相法制备玉米根质膜的鉴定[J].生物化学杂志,1997,13:686-690.
[24]Fischer-Schliebs E,Ball E,Bemdt E,et al.Differential immunological cross-reactions with antisera against the V-ATPase of Kalanchoe daigremontiana reveal structural differences of V-ATPase subunits of different plant species[J].Biol Chem,1997,378:1131-1139.
[25]Schmidt A L,BriskinN D P.Energy transduction in tonoplast vesicles from red beet(Beta vulgaris L.) storage tissue: H+/substrate stoichiometries for the H+-ATPase and H+-PPase[J].Arch Biochem Biophys,1993,1:165-173.
[26]Blumwald E,Polle R J.Salt tolerance in suspension cultures of sugar beet[J].Plant Physiol,1987,83: 884-887.
[27]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Annal Biochem,1976,72:248-254.
[28]陈兰周,刘永定,李敦海.盐胁迫对爪哇枝藻生理生化特性的影响[J].中国沙漠,2003,23(3):285-288.
[29]Luttge U.Plant cell membranes and salinity: structural,biochmical and biophsical changes[J].R Bras Fisiol Veg,1993,5(2):217-224. |
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2008, Vol. 28 
