Please wait a minute...
img

Wechat

Adv search
JOURNAL OF DESERT RESEARCH  2012, Vol. 32 Issue (6): 1681-1690    DOI:
Biology and Soil     
Effects of Ni and Cu Treatments on Contents of Antioxidants and Osmotic Adjustment Substances in Halogeton glomeratus and Peganum harmala, Two Pioneer Plants Growing in Mining Wasteland
LU Yan1, LI Xin-rong2, HE Ming-zhu2, FENG Li2, ZENG Fan-jiang1
1.Cele National Station of Observation & Research for Desert-Grassland Ecosystem in Xinjiang, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
2.Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
Download:  PDF (8513KB) 
Export:  BibTeX | EndNote (RIS)      
Abstract  

A pot experiment was conducted to study the influence of different concentrations (0, 50, 100, 200, 400 mg·kg 1) of Ni and Cu in soil on the contents of hydrogen peroxide (H2O2), ascorbic acid (ASA), glutathione (GSH), vitamin E (VE), proline and soluble sugar in leaves and roots of Halogeton glomeratus and Peganum harmala seedlings. H2O2 contents in leaves and roots of the two plant species increased with increasing Ni and Cu concentrations. In leaves of H. glomeratus, GSH content increased with increasing Ni concentration, and VE accumulated significantly in roots at higher Ni concentration (≥100 mg·kg-1). In leaves and roots of H. glomeratus under Cu stress, ASA, GSH and VE contents accumulated significantly compared with no Cu treatment, suggesting they together play an important role in weakening Cu induced oxidative damage. Under Ni stress, GSH and ASA accumulated significantly in leaves of P. harmala compared with no Ni stress, while only ASA content accumulated evidently in roots of P. harmala. Under Cu stress, GSH and ASA contents increased in leaves of P. harmala with increasing Cu concentration, and ASA content accumulated significantly in roots compared with no Cu treatment, and VE content increased markedly at 100 or 200 mg·kg 1 Cu treatment. Under Ni and Cu stress, proline and soluble sugar in H. glomeratus and P. harmala seedlings accumulated significantly compared with no Ni or no Cu treatment.

Key words:  nickel      copper      desert plants      antioxidants      osmotic adjustment substances     
Received:  12 April 2012      Published:  12 June 2012
ZTFLH:  Q945  

Cite this article: 

LU Yan1, LI Xin-rong2, HE Ming-zhu2, FENG Li2, ZENG Fan-jiang1. Effects of Ni and Cu Treatments on Contents of Antioxidants and Osmotic Adjustment Substances in Halogeton glomeratus and Peganum harmala, Two Pioneer Plants Growing in Mining Wasteland. JOURNAL OF DESERT RESEARCH, 2012, 32(6): 1681-1690.

URL: 

http://www.desert.ac.cn/EN/     OR     http://www.desert.ac.cn/EN/Y2012/V32/I6/1681

[1]刘小宁,马剑英,张慧文.植物修复技术在土壤重金属污染中应用的研究进展[J].中国沙漠,2009,29(5):859-865.

[2]廖晓勇,陈同斌,武斌,等.典型矿业城市的土壤重金属特征与复合污染评价——以“镍都”金昌市为例[J].地理研究,2006,25(5):843-852.

[3]廖晓勇,陈同斌,阎秀兰,等.金昌镍铜矿区植物的重金属含量特征与先锋植物筛选[J].自然资源学报,2007,22(3):486-495.

[4]Gonnelli C,Galardi F,Gabbrielli R.Nickel and copper tolerance and toxicity in three Tuscan populations of Silene paradoxa[J].Physiologia Plantarum,2001,113:507-514.

[5]刘玉冰,李新荣,谭会娟,等.荒漠地区两种生态型芦苇叶片的抗氧化生理特性分析[J].中国沙漠,2011,31(2):277-281.

[6]张亚娟,谢忠奎,赵学勇,等.水分胁迫对东方百合光合特性、叶绿素荧光参数及干物质积累的影响[J].中国沙漠,2011,31(4):884-888.

[7]黄海霞,王刚,陈年来.荒漠灌木逆境适应性研究进展[J].中国沙漠,2010,30(5):1060-1067.

[8]Gajewska E,Skodowska M.Effect of nickel on ROS content and antioxidative enzyme activities in wheat leaves[J].BioMetals,2007,20:27-36.

[9]Khatun S,Ali M B,Hahn E,et al.Copper toxicity in Withania somnifera:Growth and antioxidant enzymes responses of in vitro grown plants[J].Environmental and Experimental Botany,2008,64:279-285.

[10]Shah K,Kumar R G,Verma S,et al.Effect of cadmium on lipid peroxidation,superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings[J].Plant Science,2001,161:1135-1144.

[11]Gajewska E,Skodowska M.Relations between tocopherol,chlorophyll and lipid peroxides contents in shoots of Ni-treated wheat[J].Journal of Plant Physiology,2007,164:364-366.

[12]Kukkola E,Rautio P,Huttunen S.Stress indications in copper- and nickel-exposed Scots pine seedlings[J].Environmental and Experimental Botany,2000,43:197-210.

[13]Collin V C,Eymery F,Genty B,et al.Vitamin E is essential for the tolerance of Arabidopsis thaliana to metal-induced oxidative stress[J].Plant,Cell and Environment,2008,31:244-257.

[14]Sharma S S,Dietz K J.The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress[J].Journal of Experimental Botany,2006,57:711-726.

[15]左利萍,李毅,焦健.水分梯度对不同地理种群红砂叶片生理特征比较研究[J].中国沙漠,2009,29(3):514-518.

[16]Lu Y,Li X R,He M Z,et al.Seedlings growth and antioxidative enzymes activities in leaves under heavy metal stress differ between two desert plants:a perennial (Peganum harmala) and an annual (Halogeton glomeratus) grass[J].Acta Physiologiae Plantarum,2010,32:583-590.

[17]雷冬梅,段昌群,张红叶.矿区废弃地先锋植物齿果酸模在Pb、Zn污染下抗氧化酶系统的变化[J].生态学报,2009,29(10):5417-5423.

[18]Sergiev I,Alexieva V,Karanov E.Effect of spermine,atrazine and combination between them on some endogenous protective systems and stress markers in plants[J].Comptes Rendus de I’ Académie Bulgare Sciences,1997,51:121-124.

[19]张殿忠,汪沛洪,赵会贤.测定小麦叶片游离脯氨酸含量的方法[J].植物生理学通讯,1990,4:62-65.

[20]Dobois M K A,Gilles J K,Hamilton P A,et al.Colorimetric method for determination of sugars and related substrates[J].Analytical Chemistry,1956,28:350-356.

[21]Jouili H,EI Ferjani E.Changes in antioxidant and lignifying enzyme activities in sunflower roots (Helianthus annuus L.) stressed with copper excess[J].Comptes Rendus Biologies,2003,326:639-644.

[22]Ke W S,Xiong Z T,Xie M J,et al.Accumulation,subcellular localization and ecophysiological responses to copper stress in two Daucus carota L.populations[J].Plant and Soil,2007,292:291-304.

[23]刘登义,王友保,张徐祥,等.污灌对小麦幼苗生长及活性氧代谢的影响[J].应用生态学报,2002,13(10):1319-1322.

[24]鲁艳,李新荣,何明珠,等.重金属对盐生草光合生理生长特性的影响[J].西北植物学报,2011,31(2):370-376.

[25]鲁艳,李新荣,何明珠,等.不同浓度Ni、Cu处理对骆驼蓬光合作用和叶绿素荧光特性的影响[J].应用生态学报,2011,22(4):936-942.

[26]Noctor G,Foyer C H.Ascorbate and glutathione:Keeping active oxygen under control[J].Annual Review of Plant Physiology and Plant Molecular Biology,1998,49:249-279.

[27]Serbinova E A,Packer L.Antioxidant properties of α-tocopherol and α-tocotrienol[J].Methods in Enzymology,1994,234:354-366.

[28]Fryer M J.The antioxidant effects of thylakoid vitamin E (α-tocopherol)[J].Plant,Cell and Environment,1992,15:381-392.

[29]Packer L,Weber S U,Rimbach G.Molecular aspects of alpha-tocotrienol antioxidant action and cell signaling[J].Journal of Nutrition,2001,131:369S-373S.

[30]Larson R A.The antioxidants of higher plants[J].Phytochemistry,1988,27:969-978.

[31]Hare P D,Cress W A.Metabolic implications of stress-induced praline accumulation in plants[J].Plant Growth Regulation,1997,21:79-102.

[32]王蕊,孙广玉.干旱对大豆叶片葫芦巴碱含量和渗透调节的影响[J].中国沙漠,2010,30(3):552-555.

[33]张有福,陈拓,费贵清,等.盐度对三种荒漠植物渗透调节物质积累影响的研究[J].中国沙漠,2007,27(5):787-790.

[34]Metha S K,Gaur J P.Heavy-metal-induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris[J].New phytologist,1999,143:253-257.

[35]Matysik J,Bhalu B,Mohanty P.Molecular mechanism of quenching of reactive oxygen species by proline under stress in plants[J].Current Science,2002,82:525-532.

[36]李合生.现代植物生理学[M].北京:高等教育出版社,2001:630-436.

[37]Guo T R,Zhang G P,Zhang Y H.Physiological changes in barley plants under combined toxicity of aluminum,copper and cadmium[J].Colloids and Surface B:Biointerfaces,2007,57:182-188.

[38]Roitto M,Rautio P,Julkunen-Tiitto R,et al.Changes in the concentrations of phenolics and photosynthates in Scots pine (Pinus sylvestris L.) seedlings exposed to nickel and copper[J].Environmental Pollution,2005,137:603-609.

[39]孙赛初,王焕校,李启任.水生维管植物受镉污染后的生理变化及受害机制初探[J].植物生理学报,1985,11(2):113-121.

No Suggested Reading articles found!