Optimization of Proliferation Medium for the Stem of Wild Nitraria sibirica with the Response Surface Method

doi:10.7522/j.issn.1000-694X.2014.00214

JOURNAL OF DESERT RESEARCH

2015, Vol. 35

Issue (6): 1579-1583 DOI: 10.7522/j.issn.1000-694X.2014.00214

Optimization of Proliferation Medium for the Stem of Wild Nitraria sibirica with the Response Surface Method

Zhang Yanping¹, Zhao Wei², Luo Wanyin³, Dong Zhibao³

1. Institute of Biotechnology, Gansu Academy of Agricultural Science, Lanzhou 730070, China;
2. Crop Institute, Gansu Academy of Agricultural Science, Lanzhou 730070, China;
3. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China

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Abstract In order to improve the proliferation index of wild Nitraria sibirica during tissue culture, we selected the young stem segments on aseptic seedling of wild nitraria as the explant to optimize the proliferation induced with the response surface method. On the basis of one-factor proliferation test which were added different concentrations of NAA, IBA, IAA and 6-BA, combinated in medium, and according to the central composite experimental design principles, the response surface method with 2 factors and 5 levels was adopted to optimize the concentrations of 6-BA and IBA which were selected through previous test. The results showed that the best proliferation medium was MS+6-BA(0.22 mg·L^-1)+IBA(0.55 mg·L^-1). In this medium, the actual proliferation coeffcient was up to 3.90. The optimized medium increased proliferation coeffcient 3.63 which was the best result of one-factor tests to 3.90. This provided guarantee for the stable proliferation in tissue culture of wild nitraria and the follow-up study on combinating with Cynomorium in plant tissue culture environment.

Key words: response surface method Nitraria sibirica stem proliferation

Received: 06 November 2014 Published: 20 November 2015

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	Articles by authors
	Zhang Yanping
	Zhao Wei
	Luo Wanyin
	Dong Zhibao

Cite this article:

Zhang Yanping, Zhao Wei, Luo Wanyin, Dong Zhibao. Optimization of Proliferation Medium for the Stem of Wild Nitraria sibirica with the Response Surface Method. JOURNAL OF DESERT RESEARCH, 2015, 35(6): 1579-1583.

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http://www.desert.ac.cn/EN/10.7522/j.issn.1000-694X.2014.00214 OR http://www.desert.ac.cn/EN/Y2015/V35/I6/1579

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