Gene Transcriptional Expression in Reaumuria soongorica under Combined Stress of Drought and UV-B Radiation

doi:10.7522/j.issn.1000-694X.2016.00167

JOURNAL OF DESERT RESEARCH ›› 2017, Vol. 37 ›› Issue (4): 705-713.DOI: 10.7522/j.issn.1000-694X.2016.00167

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Gene Transcriptional Expression in Reaumuria soongorica under Combined Stress of Drought and UV-B Radiation

Liu Dan^1,2, Liu Yubing¹, Zhang Wenli^1,2

1. Shapotou Desert Research & Experiment Station/Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China;
2. University of Chinese Academy of Science, Beijing 100049, China

Received:2016-11-02 Revised:2016-12-05 Online:2017-07-20 Published:2017-07-20

Abstract

Abstract: Desert plants are subjected to multiple environmental factors in their natural habitats.However,little information is available about the mechanism of plant response to multiple stress factors at the same time.In this study,we analyzed the gene expression of desert plant Reaumuria soongorica in response to drought stress,enhanced UV-B radiation and their combination of the two at the transcription level by using the digital gene expression profiling technique.The results showed that differentially expressed genes (DEGs) classified into two groups:up-regulated genes and down-regulated genes.Compared to the control group,the numbers of up-regulated genes were less than that of down-regulated genes in stress treatment groups.Whereas compared to the single factor stress,numbers of total DEGs and up-regulated genes increased significantly in combined stress.The expression profiles between drought stress and UV-B radiation were more similar than that of between combined and single factor,but there still had 356 up-regulated and 248 down-regulated genes induced specifically by combined stress.For the expressed amount of DEGs,most of the gene expression changed from 2 to 5 fold,and also found some highly expressed genes (more than 100 fold).Analysis of Gene Ontology function enrichment and kyoto encyclopedia of genes and genomes pathway enrichment showed that some biological processes such as carbon fixation expressed significantly in combined stress than in single stress.Different metabolic pathways showed different expression levels in response to different stresses.The results suggested that there were great difference in the response mechanisms of plant between single factor and combined stress at gene transcription level.

Key words: Reaumuria soongorica, drought stress, UV-B radiation, two factors stress, digital gene expression profiling

CLC Number:

Q945.79

Liu Dan, Liu Yubing, Zhang Wenli. Gene Transcriptional Expression in Reaumuria soongorica under Combined Stress of Drought and UV-B Radiation[J]. JOURNAL OF DESERT RESEARCH, 2017, 37(4): 705-713.

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Gene Transcriptional Expression in Reaumuria soongorica under Combined Stress of Drought and UV-B Radiation

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