Construction and Characterization of Enriched Microsatellite Library from Desert Plant Reaumuria soongarica

Yin Hengxia; Shi Yong; Zhang Wen; Yan Xia; Ma Xiaofei

doi:10.7522/j.issn.1000-694X.2016.00015

Journal of Desert Research >

2016 , Vol. 36 >Issue 2: 374 - 382

DOI: https://doi.org/10.7522/j.issn.1000-694X.2016.00015

Construction and Characterization of Enriched Microsatellite Library from Desert Plant Reaumuria soongarica

Yin Hengxia ,
Shi Yong ,
Zhang Wen ,
Yan Xia ,
Ma Xiaofei

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1. Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2016-01-09

Revised date: 2016-02-24

Online published: 2016-03-20

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Abstract

The present study used the method of magnetic beads enrichment to build enriched microsatellite sequence library and analyzed the characteristics of library through sequencing results.The results showed that 13 922 microsatellite sequences were obtained with 88% perfect, 8% imperfect and 2% compound repeat motifs.Of these microsatellite loci, five type repeat primitives were presented, including prominent 60.7% three base primitive with (GGT)n, (TGG) n and (ACC)n, and 38.6% two base primitive with (AC)n, (GT)n and (CA)n. And the repeat time was mainly between 5 and 10, and declined as exceeding 30 times. Total 62 pairs of efficient PCR primers and 12 pairs of microsatellite primers have been screened by designing the primers with the 100 random microsatellite sequence and PCR screening with templates in populations with distant genetic and physical distance. Here, we revealed that the method of magnetic beads enrichment was high efficient and feasible for the development of Reaumuria soongarica genome microsatellite sequence. The present investigation would provide informative support and efficient genetic molecular marker for the genetic evolution and germplasm resources protection of Reaumuria soongarica.

Key words： Reaumuria soongarica; microsatellite; magnetic beads; enriched library

Cite this article

Yin Hengxia , Shi Yong , Zhang Wen , Yan Xia , Ma Xiaofei . Construction and Characterization of Enriched Microsatellite Library from Desert Plant Reaumuria soongarica[J]. Journal of Desert Research, 2016 , 36(2) : 374 -382 . DOI: 10.7522/j.issn.1000-694X.2016.00015

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