Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata

Wu Nan; Shi Yingwu; Zhu Bingjian; Zhang Yuanming

doi:10.7522/j.issn.1000-694X.2015.00177

Journal of Desert Research >

2016 , Vol. 36 >Issue 3: 718 - 725

DOI: https://doi.org/10.7522/j.issn.1000-694X.2015.00177

Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata

Wu Nan ,
Shi Yingwu ,
Zhu Bingjian ,
Zhang Yuanming

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1. Key laboratory of Biogeography and Bioresource in Arid land,Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
2. Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2015-10-14

Revised date: 2015-11-23

Online published: 2016-05-20

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Abstract

Stipagrostis pennata is a typical pioneer psammophyte species distribute in deserts of the Junggar Basin in Northern Xinjiang. This psammophyte is resistant to high temperatures, drought, and sandstorms. The presence of rhizosheath makes its ecological function stronger. Rhizosheaths are structures composed of mucilage secreted from plants and adherent soil microbes that form a hard sand cylinder around the root. Our previous study showed that rhizosheath microhabitats were favorable environment for the growth and development of microorganisms. This study focused on investigating the special microbial diversity in the rhizosheath microhabitats. The results showed that: (1) Using three different medium, 17 strains were isolated from rhizosheath microhabitats. Phylogenetic analysis of partia1 16S rDNA sequences showed that these strains clustered into 3 phyla, 5 classes, 5 orders, 8 families and 8 genera via Ribosomal Database Project (RDP) classifier. The dominant genera were Bacillus (23.53%). (2) Further classification of these strains was achieved via Basic Local Alignment Search Tool (BLAST) Homology analysis of the partial 16S rDNA against the EzTaxon-e database. BLAST results yielded 13 Operational Taxonomic Units (OTUs) and no novel species whose sequence similarity was lower than 97% compare to the species in the EzTaxon-e database. Most of these OTUs belonged to the genus Bacillus(23.08%). (3) Biological Eco technology showed that both the microbial metabolic activity and diversity were higher in the rhizosheaths than that of surrounding soil. It implied that bacteria in the rhizosheaths could well use the carbon substrates. To further discuss the relationship between the exudation from the plant and microbe, we will further investigate not only the influence of root exudation on the growth of dominant species of the microbe in the rhizosheaths, but also their soil binding properties, their mix-effect to the character of rhizosheaths.

Key words： ribosomal database project (RDP) classifier; EzTaxon-e database; Shannon-Wiener diversity index; biolog-eco technology

Cite this article

Wu Nan , Shi Yingwu , Zhu Bingjian , Zhang Yuanming . Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata[J]. Journal of Desert Research, 2016 , 36(3) : 718 -725 . DOI: 10.7522/j.issn.1000-694X.2015.00177

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