Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata

doi:10.7522/j.issn.1000-694X.2015.00177

JOURNAL OF DESERT RESEARCH ›› 2016, Vol. 36 ›› Issue (3): 718-725.DOI: 10.7522/j.issn.1000-694X.2015.00177

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Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata

Wu Nan¹, Shi Yingwu², Zhu Bingjian^1,3, Zhang Yuanming¹

1. Key laboratory of Biogeography and Bioresource in Arid land,Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
2. Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2015-10-14 Revised:2015-11-23 Online:2016-05-20 Published:2016-05-20

Abstract

Abstract:

Stipagrostis pennata is a typical pioneer psammophyte species distribute in deserts of the Junggar Basin in Northern Xinjiang. This psammophyte is resistant to high temperatures, drought, and sandstorms. The presence of rhizosheath makes its ecological function stronger. Rhizosheaths are structures composed of mucilage secreted from plants and adherent soil microbes that form a hard sand cylinder around the root. Our previous study showed that rhizosheath microhabitats were favorable environment for the growth and development of microorganisms. This study focused on investigating the special microbial diversity in the rhizosheath microhabitats. The results showed that: (1) Using three different medium, 17 strains were isolated from rhizosheath microhabitats. Phylogenetic analysis of partia1 16S rDNA sequences showed that these strains clustered into 3 phyla, 5 classes, 5 orders, 8 families and 8 genera via Ribosomal Database Project (RDP) classifier. The dominant genera were Bacillus (23.53%). (2) Further classification of these strains was achieved via Basic Local Alignment Search Tool (BLAST) Homology analysis of the partial 16S rDNA against the EzTaxon-e database. BLAST results yielded 13 Operational Taxonomic Units (OTUs) and no novel species whose sequence similarity was lower than 97% compare to the species in the EzTaxon-e database. Most of these OTUs belonged to the genus Bacillus(23.08%). (3) Biological Eco technology showed that both the microbial metabolic activity and diversity were higher in the rhizosheaths than that of surrounding soil. It implied that bacteria in the rhizosheaths could well use the carbon substrates. To further discuss the relationship between the exudation from the plant and microbe, we will further investigate not only the influence of root exudation on the growth of dominant species of the microbe in the rhizosheaths, but also their soil binding properties, their mix-effect to the character of rhizosheaths.

Key words: ribosomal database project (RDP) classifier, EzTaxon-e database, Shannon-Wiener diversity index, biolog-eco technology

CLC Number:

S154.3

Wu Nan, Shi Yingwu, Zhu Bingjian, Zhang Yuanming. Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata[J]. JOURNAL OF DESERT RESEARCH, 2016, 36(3): 718-725.

References

[1] 韩彩霞,王建明,邱菊辉,等.羽毛针禾(Stipagrostis pennata)营养器官解剖结构及其生态适应性特征[J].干旱区研究,2010,27(5):755-759.
[2] 马大伟,刘彤,赵新俊,等.羽毛针禾种群中尺度的分布格局及利它作用[J].应用与环境生物学报,2010,16(6):753-758.
[3] 朱丽洁,王绍明,夏军,等.羽毛针禾(Stipagrostis pennata) 克隆构型及不同环境中的分株种群特征[J].干旱区研究,2012,29(5):770-775.
[4] 罗丽朦,王丽学,秦立刚,等.多糖和土壤团聚体对扁穗冰草根鞘形成的影响[J].生态学报,2014,34(17):4859-4865.
[5] 邱东,吴楠,张元明,等.根鞘微生境对羽毛针禾沙生适应性的生态调节[J].中国沙漠,2012,32(6):1647-1654.
[6] 胡可,王利宾.Biolog微平板技术在土壤微生态研究中的应用[J].土壤通报,2007,38(4):819-821.
[7] Kirk J L,Beaudette L A,Hart M,et al.Methods of studying soil microbial diversity[J].Journal of Microbiological Methods,2004,58:169-188.
[8] 林先贵,陈瑞蕊,胡君利.土壤微生物资源管理、应用技术与学科展望[J].生态学报,2010,2010(24):7029-7037.
[9] Othman A A,Amer W M,Fayez M,et al.Rhizosheath of sinai desert plants is a potential repository for associative diazotrophs[J].Microbiological Research,2004,159(3):285-293.
[10] Bergmann D,Zehfus M,Zierer L,et al.Grass rhizosheath:Associated bacterial communities and potential for nitrogen fixation[J].Western North American Naturalist,2009,69(1):105-114.
[11] George T S,Brown L K,Ramsay L,et al.Understanding the genetic control and physiological traits associated with rhizosheath production by barley (Hordeum vulgare)[J].New Phytologist,2014,203(1):195-205.
[12] 郭洪旭,王雪芹,盖世广,等.古尔班通古特沙漠腹地半固定沙垄顶部风沙运动规律[J].干旱区地理,2010,33(6):954-961.
[13] 张立运,陈昌笃.论古尔班通古特沙漠植物多样性的一般特点[J].生态学报,2002,22(11):1923-1932.
[14] 张元明,王雪芹.荒漠地表生物土壤结皮形成与演替特征概述[J].生态学报,2010,30(16):4484-4492.
[15] 王华荣,彭桂香,张国霞,等.糖蜜草(Melinis minutiflora Beauv.)内生固氮菌分离鉴定[J].生态学报,2006,26(8):2566-2571.
[16] 何志祥,朱凡.雪峰山不同海拔梯度土壤养分和微生物空间分布研究[J].中国农学通报,2011,27(31):73-78.
[17] 姚如斌,吴小芹.高效解磷细菌与菌根真菌菌剂交互作用对杨树的促生效应[J].南京林业大学学报:自然科学版,2012,36(5):170-173.
[18] Wand Q,Garrity G M,Tiedje J M,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Applied and Environmental Microbiology,2007,73(16):5261-5267.
[19] Kim O S,Cho Y J,Lee K,et al.Introducing EzTaxon-e:a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species[J].International Journal of Systematic and Evolutionary Microbiology,2012,62:716-721.
[20] 靳正忠,雷加强,李生宇,等.塔里木沙漠公路防护林土壤微生物活性分异特征[J].应用生态学报,2013,24(9):2464-2470.
[21] 安晶,吴楠,张元明.沙土灭菌对羽毛针禾(Stipagrostis pennata)种子萌发、幼苗生长及根鞘形成的影响[J].中国沙漠,2016,36(2):399-405.
[22] 李晓君.五种共生菌化学成分与生物活性研究[D],陕西杨凌:西北农林科技大学.
[23] 武佳蕊,王宏伟,谢星光,等.植物内生菌影响土壤微生物区系的研究进展[J].中国生态农业学报,2014,22(11):1259-1266.
[24] 秦雯娜,徐新磊,郭立忠,等.地衣芽孢杆菌XJ-2菌株产胞外多糖发酵条件的优化及其抗氧化活性研究[J].四川农业大学学报,2014,32(2):165-171.
[25] 聂刚,陈卫民,韦革宏.神木地区耐旱灌木和草本豆科植物根瘤菌遗传多样性[J].应用生态学报,2014,25(6):1674-1680.
[26] Ge F,Zhou L Y,Wang Y,et al.Hydrolysis of the neonicotinoid insecticide thiacloprid by the N₂-fixing bacterium Ensifer meliloti CGMCC 7333[J].International Biodeterioration & Biodegradation,2014,93:10-17.
[27] Mart nez R,Espejo A,Sierra M,et al.Co-inoculation of Halomonas maura and Ensifer meliloti to improve alfalfa yield in saline soils[J].Applied Soil Ecology,2015,87:81-86.
[28] 李丽丽,郎敬,杨洪一,等.大豆根际解磷菌的鉴定[J].江苏农业科学,2014,42(8):363-365.
[29] 崔静岚,陈晨,秦智慧,等.嗜热菌对有机污染物的降解及其应用研究进展[J].应用生态学报,2012,23(11):3218-3226.
[30] 蒋西然,刘纪文,李文利.嗜热菌在纤维素乙醇中的研究进展及应用前景[J].生命科学研究,2010,14(5):449-455.
[31] 张馨月,王灿,季民.嗜热菌生物过滤技术处理高温废气的研究进展[J].化工环保,2011,31(1):34-37.
[32] 曹成有,姚金冬,韩晓姝,等.科尔沁沙地小叶锦鸡儿固沙群落土壤微生物功能多样性[J].应用生态学报,2011,22(9):2309-2315.
[33] 李卓仑,邵孔兰,宁凯,等.阿拉善高原沙漠地区植物钙质根管的矿物组成特征[J].中国沙漠,2015,35(6):1483-1488.
[34] 吴林坤,林向民,林文雄.根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J].植物生态学报,2014(3):298-310.
[35] Chaparro J M,Badri D V,Bakker M G,et al.Root exudation of phytochemicals in arabidopsis follows specific patterns that are developmentally programmed and correlate with soil microbial functions[J].Plos One,2013,8(2):e55731.
[36] 苑亚茹,韩晓增,李禄军,等.低分子量根系分泌物对土壤微生物活性及团聚体稳定性的影响[J].水土保持学报,2011,25(6):96-99.
[37] Watt M,Mccully M E,Jeffree C E.Plant and bacterial mucilages of the maize rhizosphere: Comparison of their soil binding properties and histochemistry in a model system[J].Plant and Soil,1993,151(2):151-165.
[38] Traoré O,Groleau-renaud V,Plantureux S,et al.Effect of root mucilage and modelled root exudates on soil structure[J].European Journal of Soil Science,2000,51(4):575-581.
[39] Wu H W,Haig T,Pratley J,et al.Allelochemicals in wheat (Triticum aestivum L.):Cultivar difference in the exudation of phenolic acids[J].Journal of Agricultural and Food Chemistry,2001,49(8):3742-3745.
[40] 杨阳,刘守伟,潘凯,等.分蘖洋葱根系分泌物对黄瓜幼苗生长及根际土壤微生物的影响[J].应用生态学报,2013,24(4):1109-1117.

Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata

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