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JOURNAL OF DESERT RESEARCH  2013, Vol. 33 Issue (3): 710-716    DOI: 10.7522/j.issn.1000-694X.2013.00102
Biology and Soil     
Culture-dependent Bacteria Diversity of Lichen Crusts in the Gurbantunggut Desert
WU Nan1, ZHANG Yuan-ming1, PAN Hui-xia1, Qiu dong2
1.Key laboratory of Biogeography and Bioresource in Arid land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011,China;
2.College of Pratacultural and Environmental Science, Xinjiang Agricultural University, Urumqi 830052, China
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Abstract  

Lichen crusts is the main type biological soil crusts(BSCs) in the southern part of the Gurbantunggut Desert and served as one of the important nitrogen resource. It is also one of the sensitive biomonitors of desert ecosystem health, habitat heterogeneity and human induced disturbances. Using three different medium, 18 bacterial strains were isolated from lichen crusts. Phylogenetic analysis of partia1 16S rDNA sequences showed that: (1) These strains were clustered into 3 phyla, 3 classes, 3 orders, 6 families and 6 genera via Ribosomal Database Project (RDP) classifier. The phyla Proteobacteria, Actinobacteria and Firmicutes each owned 6 of the 18 strains. The dominant genera were Acinetobacter (33%), Streptomyces (28%) and Bacillus (22%). (2) Further classification of these strains was achieved via Basic Local Alignment Search Tool (BLAST) Homology analysis of the partial 16S rDNA against the EzTaxon-e database. BLAST results yielded 14 Operational Taxonomic Units (OTUs) and no novel species whose sequence similarity was lower than 97% compare to the species in the EzTaxon-e database.were obtained. Most of these OTUs belonged to the genus Streptomyces (35.71%). (3) The Shannon-Wiener diversity index was 2.5532, a value lower than that of BSCs for the Hunsandake Desert. (4) These culture-dependent bacteria might play important roles in fixing sand particles, releasing plant-available nutrients from soil minerals and inhibiting the growth of plant pathogens, thereby promoting BSCs development.

Key words:  prokaryote      Ribosomal Database Project (RDP) classifier      EzTaxon-e database      Shannon-Wiener diversity index      Streptomyces     
Received:  19 June 2012      Published:  10 August 2012
ZTFLH:  Q938.1  

Cite this article: 

WU Nan1, ZHANG Yuan-ming1, PAN Hui-xia1, Qiu dong2. Culture-dependent Bacteria Diversity of Lichen Crusts in the Gurbantunggut Desert. JOURNAL OF DESERT RESEARCH, 2013, 33(3): 710-716.

URL: 

http://www.desert.ac.cn/EN/10.7522/j.issn.1000-694X.2013.00102     OR     http://www.desert.ac.cn/EN/Y2013/V33/I3/710

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