Aconitous acid is an important root exudate of Lanzhou lily. In order to evaluate the feasibility of aconitic acid as an allelopathic inhibitor for the prevention and treatment of lily wilt， four treatments were designed： CK （clean water treatment）， F （bacteriosis， no aconitic acid treatment）， T （1 000 mg·L^-1 aconitic acid treatment）， T+F （bacteriological treatment， 1 000 mg·L^-1 aconitic acid treatment）， and the allelopathic effect of aconitum acid on Lily wilt in Lanzhou was studied. The ex vivo culture results of Fusarium oxysporum showed that compared with F treatment， T+F treatment significantly reduced the proliferation and number of Fusarium oxysporum， and its effect was effective. Compared with F treatment， the incidence and disease index of T+F treatment were significantly reduced by 44.45% and 28.21%， respectively. The SOD， PAL， POD， PPO activity and proline content of T+F treatment increased significantly by 122.63%， 17.49%， 45.35%， 119.36% and 92.59%， respectively. Compared with CK， the APX activity and proline content of T treatment （1 000 mg·L^-1 aconitic acid treatment） were significantly increased by 142.58% and 52.1%， respectively. After principal component analysis， the order of composite indexes for different treatments was： CK>T+F >T >F. In summary， exogenous aconitine acid can reduce the incidence of lily wilt and alleviate the adverse effects of Fusarium acusia on the growth and development of lily plants， and has the possibility of being applied as an allelopathic inhibitor to the prevention and control of lily wilt.