红花岩黄芪(Hedysarum multijugum)、灌木铁线莲(Clematis fruticosa)和互叶醉鱼草(Buddleja alternifolia)幼苗适应干旱的生理特征比较

doi:10.7522/j.issn.1000-694X.2019.00065

摘要/Abstract

摘要： 以红花岩黄芪（Hedysarum multijugum）、灌木铁线莲（Clematis fruticosa）和互叶醉鱼草（Buddleja alternifolia）幼苗（3~4月龄）为试验材料，分别用水和150 mmol·L^-1 NaCl溶液浇灌至土壤水分含量达到饱和后进行自然干旱处理，比较分析低盐和无盐条件下3种灌木幼苗叶片抗旱适应的生理响应特征，并采用隶属函数法对3种灌木幼苗的抗旱性进行综合评价。结果表明：随着干旱胁迫时间延长，3种植物叶片的株高和叶面积有所增加，但增幅减少；相对含水量均有不同程度降低，丙二醛（MDA）、脯氨酸和可溶性糖含量显著增加；红花岩黄芪和灌木铁线莲的可溶性蛋白含量及超氧化物歧化酶（SOD）、过氧化物酶（POD），过氧化氢酶（CAT）活性先升后降，而互叶醉鱼草则显著升高，抗氧化酶系统相对较为稳定；红花岩黄芪和灌木铁线莲的光合色素含量逐渐减少，而互叶醉鱼草则先增加后减少。无盐和低盐条件相比，低盐下3种植物的株高和叶面积增幅相对较大，叶片相对含水量、MDA含量和光合色素含量的变化幅度较小，渗透调节物质的累积量和抗氧化酶的活性较高。隶属函数法综合分析得出，3种灌木幼苗的抗旱性：互叶醉鱼草 > 灌木铁线莲 > 红花岩黄芪，低盐环境明显促进了3种灌木的抗旱适应能力。

关键词: 幼苗, 干旱胁迫, 隶属函数法

Abstract: Three 3-month-old shrub seedlings, Hedysarum multijugum, Clematis fruticosa and Buddleja alternifolia were selected as the test materials. The shrub seedlings were divided into two groups, which were irrigated with water and 150 mM NaCl solution, and then treated with natural drought after the soil moisture was saturated. The physiological responses of the three shrub seedlings to drought under low salt and no salt conditions were determined, and the drought tolerance of the three shrub seedlings were further comprehensively evaluated with the subordinate functions. Results showed that, with the prolongation of drought stress duration, the height and leaf area of three shrub seedings increased, but the increase decrease, the leaf relative water content all decreased to certain extent, and the malondialdehyde first increased and then decreased in H. multijugum and C. fruticosa, and significantly increased in B. alternifolia, while the antioxidant enzyme system in B. alternifolia was less varied. The photosynthetic pigment content gradually decreased in H. multijugum and C. fruticosa, and first increased and then decreased in B. alternifolia. In comparison with the salt-free treatment, the height and leaf area of three shrub seedings under low salt treatment increase relatively large, the leaf relative water content, MDA contents and photosynthetic pigment content of the three shrub seedings under low salt treatment were smaller, and the accumulation of osmotic regulation substances and anti-oxidant enzyme activities were higher. Subordinate function analysis revealed that the drought tolerance of the three shrub seedlings from strong to weak was B. alternifolia, C. fruticosa and H. multijugum. Low-salt environment significantly promoted drought resistance of three shrubs.

Key words: seedling, drought stress, subordinate function methodI

中图分类号:

Q945.78

张雯莉, 朱恭, 黄文广, 张宇, 王蕾, 罗晓玲, 刘玉冰. 红花岩黄芪(Hedysarum multijugum)、灌木铁线莲(Clematis fruticosa)和互叶醉鱼草(Buddleja alternifolia)幼苗适应干旱的生理特征比较[J]. 中国沙漠, 2020, 40(3): 159-167.

Zhang Wenli, Zhu Gong, Huang Wenguang, Zhang Yu, Wang Lei, Luo Xiaoling, Liu Yubing. Physiological response characteristics of Hedysarum multijugum, Clematis fruticosa and Buddleja alternifolia seedlings to drought in semi-arid region of Northwest China[J]. Journal of Desert Research, 2020, 40(3): 159-167.

参考文献

[1] Ren M,Zhang Z,Wang X,et al.Diversity and contributions to nitrogen cycling and carbon fixation of soil salinity shaped microbial communities in Tarim Basin[J].Frontiers in Microbiology,2018,9:431.
[2] 王利界,周智彬,常青,等.盐旱交叉胁迫对灰胡杨(Populus pruinosa)幼苗生长和生理生化特性的影响[J].生态学报,2018,38(19):7026-7033.
[3] 成升魁,鲁春霞,郭金花,等.中国农业资源环境透视:问题与建议[J].科技导报,2018,36(11):13-21.
[4] Farooq M,Wahid A,Kobayashi N,et al.Plant drought stress:effects,mechanisms and management[J].Agronomy for Sustainable Development,2009,29(1):185-212.
[5] Pandey V,Shukla A.Acclimation and tolerance strategies of rice under drought stress[J].Rice Science,2015,22(4):147-161.
[6] 唐益苗,赵昌平,高世庆,等.植物抗旱相关基因研究进展[J].麦类作物学报,2009,29(1):166-173.
[7] Seki M,Umezawa T,Urano K,et al.Regulatory metabolic networks in drought stress responses[J].Current Opinion in Plant Biology,2007,10(3):296-302.
[8] Cattivelli L,Rizza F,Badeck F W,et al.Drought tolerance improvement in crop plants:an integrated view from breeding to genomics[J].Field Crops Research,2008,105(1/2):1-14.
[9] Liu W,Xiang Y,Zhang X,et al.Over-expression of a maize N-acetylglutamate kinase gene (ZmNAGK) improves drought tolerance in Tobacco[J].Frontiers in plant science,2019,9:1902.
[10] Rivero R M,Kojima M,Gepstein A,et al.Delayed leaf senescence induces extreme drought tolerance in a flowering plant[J].Proceedings of the National Academy of Sciences,2007,104(49):19631-19636.
[11] 赵杏锁,孙海博,刘一心,等.模拟干旱与盐胁迫对半枝莲种子萌发的影响[J].草业科学,2018,35(6):1496-1502.
[12] Khan M A,Gulzar S.Germination responses of Sporobolus ioclados:a saline desert grass[J].Journal of Arid Environments,2003,53(3):387-394.
[13] 赵九洲,郭绍霞.野生花卉在我国北方园林中的应用研究[J].南京林业大学学报(人文社会科学版),2004,4(1):84-88.
[14] 张毅.两种野生花卉的引种研究[D].河北保定:河北农业大学,2007.
[15] 周涛,朴永吉,林元雪.中国野生花卉资源的研究现状及展望[J].世界林业研究,2004,17(4):45-48.
[16] 贾鑫,孙窗舒,李光跃,等.干旱胁迫对蒙古黄芪生长和生理生化指标及其黄芪甲苷积累的影响[J].西北植物学报,2018,38(3):501-509.
[17] 王荷.野生花卉用于野花草地的营建初探[D].北京:北京林业大学,2009.
[18] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2015.
[19] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[20] 张立军,樊金娟.植物生理学实验教程[M].北京:中国农业大学出版社,2007.
[21] Liu Y B,Li X R,Chen G X,et al.Epidermal micromorphology and mesophyll structure of populous euphratica heteromorphic leaves at different development Stages[J].Plos One,2015,10(9):e0137701.
[22] 安玉艳,梁宗锁.植物应对干旱胁迫的阶段性策略[J].应用生态学报,2012,23(10):2907-2915.
[23] Chaves M M,Oliveira M M.Mechanisms underlying plant resilience to water deficits:prospects for water-saving agriculture[J].Journal of Experimental Botany,2004,55(407):2365-2384.
[24] 刘凯月.大叶铁线莲和荆芥的抗旱性研究[D].河北保定:河北农业大学,2018.
[25] 倪建中,王伟,郁书君,等.干旱胁迫对木棉叶片若干生理生化指标的影响[J].热带作物学报,2014,35(10):2020-2024.
[26] 邓辉茗,龙聪颖,蔡仕珍,等.不同水分胁迫对绵毛水苏幼苗形态和生理特性的影响[J].西北植物学报,2018,38(6):123-132.
[27] Xing X H,Fang C W,Li L,et al.Improved drought tolerance by α-naphthaleneacetic acid-induced ROS accumulation in two soybean cultivars[J].Journal of Integrative Agriculture,2016,15(8):1770-1784.
[28] Zhang S H,Feng X U,Sun Y M,et al.Influence of drought hardening on the resistance physiology of potato seedlings under drought stress[J].Journal of Integrative Agriculture,2018,17(2):336-347.
[29] 井大炜,邢尚军,杜振宇,等.干旱胁迫对杨树幼苗生长、光合特性及活性氧代谢的影响[J].应用生态学报,2013,24(7):1809-1816.
[30] 可静,李进,李永洁.干旱胁迫下黑果枸杞幼苗对外源水杨酸的生理响应[J].植物生理学报,2016,52(4):497-504.
[31] 赵超,王海燕,刘美珍,等.干旱胁迫下木薯茎杆可溶性糖、淀粉及相关酶的代谢规律[J].植物生理学报,2017,53(5):795-806.
[32] 张雯莉,刘玉冰,刘立超.盐胁迫对枸杞(Lycium barbarum)叶片生理特性的影响[J].中国沙漠,2018,38(2):294-299.
[33] Szabados L,Savouré A.Proline:a multifunctional amino acid[J].Trends in Plant Science,2010,15(2):89-97.
[34] Mane A V,Karadge B A,Samant J S.Salt stress induced alteration in growth characteristics of a grass Pennisetum alopecuroides[J].Journal of Environmental Biology,2011,32(6):753.
[35] 王竞红,陈鹏,陈艾,等.3种观赏草苗期对干旱胁迫的响应及抗旱性评价[J].草业科学,2019,36(5):1266-1274.
[36] Proctor M C,Smirnoff N.Rapid recovery of photosystems on rewetting desiccation-tolerant mosses:chlorophyll fluorescence and inhibitor experiments[J].Journal of Experimental Botany,2000,51(351):1695-1704.
[37] 王海珍,陈加利,韩路,等.地下水位对胡杨(Populus euphratica)和灰胡杨(Populus pruinosa)叶绿素荧光光响应与光合色素含量的影响[J].中国沙漠,2013,33(4):1054-1063.
[38] Ristic Z,David D.Chloroplast structure after water and high temperature stress in two lines of maize that differ in endogenous levels of abscises acid[J].International Journal Plant Science,1992,153:186-196.
[39] 朱永群,彭丹丹,彭燕,等.苏丹草及高丹草幼苗对干旱胁迫的生理响应与抗旱性比较[J].草业科学,2019,36(5):1361-1370.