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中国沙漠 ›› 2015, Vol. 35 ›› Issue (6): 1579-1583.DOI: 10.7522/j.issn.1000-694X.2014.00214

• 生物与土壤 • 上一篇    下一篇

利用响应面法优化野生白刺(Nitraria sibirica)茎段增殖培养基

张艳萍1, 赵玮2, 罗万银3, 董治宝3   

  1. 1. 甘肃省农业科学院生物技术研究所, 甘肃 兰州 730070;
    2. 甘肃省农业科学院作物研究所, 甘肃 兰州 730070;
    3. 中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室, 甘肃 兰州 730000
  • 收稿日期:2014-11-06 修回日期:2014-12-12 出版日期:2015-11-20 发布日期:2015-11-20
  • 通讯作者: 董治宝(Email:zbdong@lzb.ac.cn)
  • 作者简介:张艳萍(1978-),女,甘肃武威人,助理研究员,主要从事分子生物学研究、病毒检测以及中药材组培研究。Email:64929217@qq.com
  • 基金资助:
    科技部农村领域国家科技计划课题(2012BAD16B0303)

Optimization of Proliferation Medium for the Stem of Wild Nitraria sibirica with the Response Surface Method

Zhang Yanping1, Zhao Wei2, Luo Wanyin3, Dong Zhibao3   

  1. 1. Institute of Biotechnology, Gansu Academy of Agricultural Science, Lanzhou 730070, China;
    2. Crop Institute, Gansu Academy of Agricultural Science, Lanzhou 730070, China;
    3. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2014-11-06 Revised:2014-12-12 Online:2015-11-20 Published:2015-11-20

摘要: 为了提高野生白刺(Nitraria sibirica)组培过程中的增殖系数,以野生白刺的无菌苗幼嫩茎段为外植体,利用响应面法对白刺茎段增殖诱导试验进行优化。在添加不同激素浓度NAA、IBA、IAA与6-BA组合的单因素增殖诱导试验基础上,根据CCD中心组合试验设计原理,采用2因素5水平的响应面法,对挑选出的6-BA和IBA两个因素浓度进行优化,得出增殖诱导最佳培养基为MS+6-BA(0.22 mg·L-1)+IBA(0.55 mg·L-1)。在此条件下,实际增殖系数可达到3.90。优化后的培养基使得增殖系数从之前单因素实验所得最高增殖系数3.63提高到了3.90。为野生白刺组培方面稳定增殖以及后续在组培环境下与锁阳接种研究提供了保障。

关键词: 响应面法, 白刺(Nitraria sibirica), 茎段, 增殖

Abstract: In order to improve the proliferation index of wild Nitraria sibirica during tissue culture, we selected the young stem segments on aseptic seedling of wild nitraria as the explant to optimize the proliferation induced with the response surface method. On the basis of one-factor proliferation test which were added different concentrations of NAA, IBA, IAA and 6-BA, combinated in medium, and according to the central composite experimental design principles, the response surface method with 2 factors and 5 levels was adopted to optimize the concentrations of 6-BA and IBA which were selected through previous test. The results showed that the best proliferation medium was MS+6-BA(0.22 mg·L-1)+IBA(0.55 mg·L-1). In this medium, the actual proliferation coeffcient was up to 3.90. The optimized medium increased proliferation coeffcient 3.63 which was the best result of one-factor tests to 3.90. This provided guarantee for the stable proliferation in tissue culture of wild nitraria and the follow-up study on combinating with Cynomorium in plant tissue culture environment.

Key words: response surface method, Nitraria sibirica, stem, proliferation

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