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中国沙漠 ›› 2009, Vol. 29 ›› Issue (4): 703-710.

• 生物土壤与生态 • 上一篇    下一篇

沙冬青根瘤菌结瘤基因nodA PCR-RFLP分析

毕江涛1,2,3 , 贺达汉1, 谢瑞梅2, 韦革宏2*   

  1. 1.宁夏大学 农学院, 宁夏 银川 750012; 2.西北农林科技大学 微生物研究中心, 陕西 杨陵 712100; 3.宁夏农业技术推广总站, 宁夏 银川 750001
  • 收稿日期:2008-10-13 修回日期:2008-12-21 出版日期:2009-07-20 发布日期:2009-07-20

PCR-RFLP Analysis of Nodulation Gene nodA of Rhizobia from Ammopiptanthus mongolicus

BI Jiang-tao1,2,3, HE Da-han1, XIE Rui-mei2, WEI Ge-hong2   

  1. 1.College of Agriculture, Ningxia University, Yinchuan 750012, China; 2.Microbial Research Center, Northwest Agriculture and Forestry University, Yanglin 712100, Shaanxi, China; 3.Ningxia Extension Service for Agricultural Techniques, Yinchuan 750001, China
  • Received:2008-10-13 Revised:2008-12-21 Online:2009-07-20 Published:2009-07-20

摘要: 利用结瘤基因nodA PCR-RFLP和nodA PCR产物序列分析技术,对36株分离自沙冬青根瘤菌菌株的共同结瘤基因nodA进行了遗传类型和系统发育关系分析。试验结果表明,36株根瘤菌nodA PCR-RFLP分析显示在80%的相似水平上聚为两个类群5个亚群;选取不同基因型代表菌株的nodA进行序列测定和系统发育关系分析表明,测试菌株CCNWNX0117、CCNWNX0068、CCNWNX0083、CCNWNX0062、CCNWNX0058与中慢生根瘤菌聚在一起,系统发育地位相近;测试菌株CCNWNX0089、CCNWNX0081、CCNWNX0064与中华根瘤菌聚在一起,有较高的序列相似性。本研究中nodA基因所揭示的沙冬青根瘤菌遗传多样性和系统发育关系说明沙冬青根瘤菌nodA基因遗传多样性较高,宿主结瘤范围较宽。

关键词: 沙冬青, 结瘤基因, 限制性片段长度多态性分析, 测序, 系统发育树

Abstract: Studies on relations between genotypes and phylogenic of 36 rhizobial strains isolated from Ammopiptanthus mongolicus were conducted by the methods of nodA PCR-RFLP and nodA sequencing. The results of PCR-RFLP clustering analysis showed that, at the similarity of 80%, the 36 strains are clustered with 2 groups within which there are 5 subgroups. The sequencing of the representative tested strains chosen from the identified different genotypes in the trials was conducted, and the analysis on phylogenic relations indicated that the tested strains of CCNWNX0117, CCNWNX0068, CCNWNX0083, CCNWNX0062, and CCNWNX0058 clustered with Mesorhizobium, and that the phylogenetic status is very close; meanwhile the tested strains of CCNWNX0089, CCNWNX0081, CCNWNX0064 clustered with Sinorizobium, the similarity of sequences is high. The studies indicated that the genetic diversity of nodA gene is relatively rich and has a broad adaptability to the potential hosts in nodulation.

Key words: Ammopiptanthus mongolicus, Nodulation gene, PCR-RFLP, sequencing, phylogenetic tree

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