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JOURNAL OF DESERT RESEARCH  2013, Vol. 33 Issue (1): 118-125    DOI: 10.7522/j.issn.1000-694X.2013.00017
Biology and Soil     
Impact of Single Ni and Cu Treatment on Activities of Antioxidative Enzymes of Halogeton glomeratus, A Pioneer Plant Growing in Ni/Cu Mining Wasteland
LU Yan1, LI Xin-rong2, HE Ming-zhu2, ZHAO Xin2, LI Xiao-jun2, WANG Jin2, ZENG Fan-jiang1
1.Cele National Station of Observation & Research for Desert-Grassland Ecosystem in Xinjiang, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;
2.Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
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Abstract  

Halogeton glomeratus seedlings planted in plastic pots in a greenhouse were treated separately with NiSO4·5H2O and CuSO4·6H2O solutions at 0, 50, 100, 200, and 400 mg·kg-1 concentration levels (computed with single Ni or Cu element). Impacts of the treatments on Ni, Cu accumulation, lipid peroxidation and antioxidative enzymes system in the roots and leaves of H. glomeratus were examined. The results showed that: ① The Ni, Cu contents in the leaves and roots of H. glomeratus both increased in a dose-dependent manner, and more in roots; ② The Malondialdehyde (MDA) contents significantly increased in leaves and roots of H. glomeratus with increasing Ni and Cu concentrations, indicating a lipid peroxidation aggravation; ③ In the leaves of H. glomeratus, only ascorbate peroxidase (APX) activity increased with increasing Ni concentrations, indicating that APX could contribute to protection against Ni induced oxidative stress. While in the roots of H. glomeratus, the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), APX, and glutathione reductase (GR) enhanced with increasing Ni concentrations, suggesting that they played important roles in weakening Ni induced oxidative damages; ④ Under Cu stress, the activities of SOD and APX in leaves of H. glomeratus ascended compared with control level, and they could be effective in lessening Cu induced oxidative damage; ⑤ In the roots of H. glomeratus, as a whole, the five antioxidative enzymes activities increased with increasing Cu concentrations, thus might protect seedlings from Cu induced oxidative damage by exerting a certain antioxidant function.

Key words:  Ni      Cu      Halogeton glomeratus      antioxidative enzymes      lipid peroxidation     
Received:  20 October 2012      Published:  20 January 2013
ZTFLH:  Q945  

Cite this article: 

LU Yan1, LI Xin-rong2, HE Ming-zhu2, ZHAO Xin2, LI Xiao-jun2, WANG Jin2, ZENG Fan-jiang1. Impact of Single Ni and Cu Treatment on Activities of Antioxidative Enzymes of Halogeton glomeratus, A Pioneer Plant Growing in Ni/Cu Mining Wasteland. JOURNAL OF DESERT RESEARCH, 2013, 33(1): 118-125.

URL: 

http://www.desert.ac.cn/EN/10.7522/j.issn.1000-694X.2013.00017     OR     http://www.desert.ac.cn/EN/Y2013/V33/I1/118

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